Labshake search
Citations for Roche :
51 - 100 of 225 citations for L Cysteine S Methyl S Methyl D3 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: All qPCR reactions were performed with a LightCyler 1.3 Real-Time PCR system (Roche S/N: 140 6143). The MgCl2 concentration (1-3 mM ...
-
bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 s off cycle using a total of 30 minutes) in the presence of DNase I (Roche, Basel, CH) and cOmplete EDTA-free protease inhibitor (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using a One-step BrightGreen RT-qPCR Kit (Abm, G471-S) following the manufacturer’s protocol in the LightCycler 96 Real-Time PCR System (Roche). Isocitrate dehydrogenase (ICDH ...
-
bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
-
bioRxiv - Microbiology 2022Quote: Cysteines were reduced using 10 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated in the presence of 25 mM iodoacetamide (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... Triplicate qPCR reactions of the Telomeric (T) product and the Single copy gene (S) (HBB) were amplified using a Roche LightCycler 480 II (Roche) using the following programs ...
-
bioRxiv - Physiology 2019Quote: ... Serum Vitamin D (S-25-OH-D) levels were measured in samples collected before and after the intervention using a electrochemiluminescence immunoassay (Roche Cobas Vitamin D total assay ...
-
bioRxiv - Cell Biology 2022Quote: ... 30 s at 72°C) and final extension 5 min at 72°C using KAPA Taq DNA polymerase (KAPA BIOSYSTEMS). PCR products were visualized on 2% agarose gels containing SYBR Safe DNA gel stain (Invitrogen) ...
-
bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
-
bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
-
bioRxiv - Microbiology 2021Quote: ... and transfected with plasmid pIIIH5red-SARS-2-S DNA using X-tremeGENE HP DNA Transfection Reagent (Roche Diagnostics, Penzberg, Germany) according to the manual ...
-
bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
-
Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The antisense RNA probes corresponding to the 3’ s-end portion of glnA CDS were prepared by the DIG RNA Labeling Kit (Roche).
-
bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
-
bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction was stopped by the addition of 500 ng of plasmid (size > 10 kb) and 0.1 pmol of ATP-γ-S tetralithium salt (10102342001-Roche, Sigma) followed by incubation for 30 min at 30°C ...
-
bioRxiv - Plant Biology 2023Quote: ... the aerial parts were dipped for 30 s in 1 ml of 0.01% (v/v) Tween-20 aqueous solution containing 50 µM MG132 (Roche Sigma-Aldrich) in DMSO ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 s at 68 °C before final extension for 60 s at 68 °C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems) ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 s at 68 °C before final extension for 60 s at 68 °C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems) ...
-
bioRxiv - Immunology 2021Quote: The measurement of anti SARS-CoV-2 neutralizing Abs was performed by electrochemiluminescence sandwich immunoassay (ECLIA) through Roche Elecsys Anti-SARS-CoV-2 S (Roche diagnostics, Switzerland). The neutralizing Ab were measured on a Cobas 601 modular analyzer (Roche diagnostics ...
-
bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
-
bioRxiv - Molecular Biology 2021Quote: ... cysteine protease inhibitors (Complete Mini™ and Pefabloc®, Roche Life Science), and 0.05% detergent solution (NP-40 Surfact-Amps™ ...
-
bioRxiv - Genomics 2022Quote: ... Positivity of SARS-CoV-2 infection was assessed both by PCR and measurement of specific antibodies (Cobas-Roche using Elecsys Anti-SARS-CoV-2 S). All patients gave their consent for participation in this study ...
-
bioRxiv - Neuroscience 2021Quote: ... medium was replaced with E6 medium (DMEM/F-12 supplemented with 64 mg/L L-ascorbic acid 2-phosphate magnesium, 14 µg/L sodium selenium, 543 mg/L sodium bicarbonate, mg/L insulin [Roche, Penzberg ...
-
bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
-
bioRxiv - Immunology 2021Quote: Approximately 200-500 ng of FFPE RNA extracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA (DV200 98-99) per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). The number of pre-capture PCR cycles was adjusted based on the quality and quantity of RNA extracted from the samples ...
-
bioRxiv - Physiology 2022Quote: ... 2 mmol/l MgCl2, 10 mmol/l NaF, 1 mmol/l PMSF, 1% Triton X-100 and Complete Protease inhibitor mixture, Roche Diagnostics) for 30 min on ice and centrifuged at 20,000 g for 10 min at 4ºC ...
-
bioRxiv - Plant Biology 2020Quote: ... consisting of MS1 with 300 mg/L carbenicillin and 5 mg/L hygromycin (Roche, Germany) (YFT1-CDS) ...
-
bioRxiv - Immunology 2019Quote: ... containing 40 mg/L Liberase (Roche) at a rate of 5 mL/min ...
-
bioRxiv - Microbiology 2021Quote: ... L-lactate dehydrogenase (Roche Applied Science) was used to construct a standard curve that demonstrated an ample linear dynamic range (r=0.998 ...
-
bioRxiv - Plant Biology 2020Quote: ... L-lactate dehydrogenase was from Roche. All other reagents were of the highest available quality.
-
bioRxiv - Immunology 2019Quote: ... and DNAse l (200U/mL, Roche), dissociated using the octoMACS dissociation program (Spin 1000rpm 36 seconds ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.1 g/L Proteinase K (Roche), 2.5 mM dNTPs (Takara) ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.1 g/L Proteinase K (Roche), 2.5 mM dNTPs (Takara) ...
-
bioRxiv - Molecular Biology 2023Quote: L-Lactate dehydrogenase (LDH) enzyme (Roche) was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2024Quote: ... L-lactate dehydrogenase (Roche Applied Science) was used to construct a standard curve that demonstrated an ample linear dynamic range (r2>0.998 ...
-
bioRxiv - Biochemistry 2021Quote: ... and 0.1 mol/L 1,4-dithiothreitol (Roche) in 0.1 mol/L Tris-HCl (pH 7.6) ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.25 g/l cOmplete protease inhibitor (Roche)] ...
-
bioRxiv - Developmental Biology 2021Quote: ... with 19.4 mg/L insulin (#11376497001; Roche), 64 mg/L L-ascorbic acid (#A8960 ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.5 mM PMSF) supplemented with 5 mg L−1 DNAse I and 0.5 g/L lysozyme (Roche, Mannheim, Germany). Cells were disrupted by sonication (3×2 min ...
-
bioRxiv - Microbiology 2022Quote: ... bacteria were washed twice in phosphate buffer saline and resuspended in 7H9 base media + 0.05% tyloxapol + 0.085% NaCl containing either 5g/L or 50g/L of fatty acid free BSA (fraction V Roche) with no glycerol nor dextrose added ...
-
bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (protein concentration at 0.35 g/L to compare with normal range 0.2-0.4 g/L range (Roche et al., 2008); cell count per mm3 was 100) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and L-lactate dehydrogenase from rabbit muscle (Roche) was used as a standard ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 20 mmol/l HEPES/NaHEPES (Roche Molecular Biochemicals ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mmol/L PMSF and 2X Protease Inhibitor (Roche)) and sheared by sonication (25% amplitude ...
-
bioRxiv - Biochemistry 2021Quote: ... L-malate dehydrogenase (MDH, from pig heart mitochondrial, Roche), glutamate dehydrogenase (GDH ...
-
bioRxiv - Biochemistry 2021Quote: Pig heart L-malate dehydrogenase (MDH2; Roche, catalogue # 10127256001) and recombinant human HSPA8 were prepared in HEPES buffer (50 mM HEPES ...
-
bioRxiv - Microbiology 2019Quote: ... albumin (5 g/L; fraction V, fatty acid-free, Roche) and tyloxapol (0.04%) ...