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Citations for Roche :
451 - 500 of 1968 citations for IL 2 Rat HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Rat monoclonal anti-HA (1:50 for IF and 1:1000 for IB; Roche); mouse monoclonal anti-α-tubulin (1:200 for IF and 1:2500 or 1:10,000 for IB ...
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bioRxiv - Microbiology 2021Quote: ... Bound primary antibodies were detected using biotin-conjugated anti-rat antibody (Roche, 1:1,000) and AlexaFluor594-conjugated streptavidin (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... GRA16HA (and other HA-tagged proteins) was detected using rat anti-HA antibodies (Roche) while GRA24MYC was detected using rabbit anti-MYC tag antibody 9E10 (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2019Quote: ... The antibodies used in this study were rat anti-HA (3F10; Roche, 1:3,000), rabbit anti-HA (715500 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies including rat anti-tubulin (MCA78G; Serotec) and mouse anti-Myc (11667149001; Roche) were used at a dilution of 1:5000 and 1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with rat anti-HA antibodies (Roche, 3F10; 0.2 μg/ml) for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... A primary antibody was used to detect HA (anti-rat, 1:200, 11867423001, Roche) and detection carried out with fluorescent-labeled secondary antibodies (goat anti-rat ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies used in this study were rat-anti-HA 3F10 (Roche, 1:3000), mouse-anti-HA 6E2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used for staining were: rat anti-HA (Roche 11 867 423 001); rabbit anti-CLIMP-63 (CKAP4 ...
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bioRxiv - Plant Biology 2020Quote: ... but using an anti-HA-HRP conjugated antibody (rat monoclonal, Roche, catalog number 12013819001) at 1:5000 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse α-HA F7 (1:1000, Santa-Cruz) rat α-HA (1:750, Roche); mouse α-β-Tubulin (1:5000 ...
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bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used: rat anti-HA (clone 3F10, Roche, 1:1000), mouse anti-HA (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... Primary antibodies used were α-GFP (mouse) and α-hemagglutinin (rat; both from Roche). Horseradish peroxidase-coupled (GE Healthcare ...
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bioRxiv - Pathology 2023Quote: ... The following antibodies were used: rat anti-HA (3F10, 1:400; Roche, Switzer land), mouse anti-Chaoptin (24B10 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in the study: rat anti-HA (Roche, Basel, Switzerland), rabbit anti-HA (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then stripped and incubated with rat anti-HA primary antibody (Roche) at 1/2000 dilution in PBST 5% milk and washed 3 times ...
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bioRxiv - Biochemistry 2023Quote: ... the blots were probed with HRP-conjugated rat anti-HA antibody (clone 3F10, Roche) at a 1:1000 dilution and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated overnight with primary antibodies: rat monoclonal anti-HA (1:1000, ROCHE), or Actin5c (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with rat α-HA (3F10, Roche), rabbit α-Myc (Cell Signaling) ...
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bioRxiv - Microbiology 2021Quote: ... 2 M CaCl2 and 2 UU/ml DNAse I (Roche) for 30 min at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Biophysics 2021Quote: ... 2 mM MgCl2) supplemented with 2 protease inhibitor tablets (Roche), 1 mM PMSF ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Cancer Biology 2020Quote: ... grade 2 (Roche), homogenizing for 20min with a plastic Pasteur pipette ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche, Indianapolis, IL, USA) using a FAST SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Th17-cell differentiation was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... in the absence (Th0) or presence (Th17) of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Th17 cell polarization was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with 30 μl pre-washed rat anti-HA Affinity Matrix (Roche) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... was used for staining Cse4-GFP and rat anti-HA (1:200 dilution: Roche, 11867423001) for Mad2-3HA staining as primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... the rats’ fasting blood glucose (FBG) was measured with a glucose meter (Roche, Basel, Switzerland). The serum levels of insulin (80-INSRT-E01) ...
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bioRxiv - Cell Biology 2019Quote: ... The antibodies used for IFA were: rat anti-HA antibody (clone 3F10; Roche, 1:100), mouse anti-AMA1 (1F9 from Alan Cowman) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The HA-tagged tdnano construct was stained using a rat α-HA primary antibody (Roche) and an Alexa Fluor 647-conjugated secondary antibody (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibody and dilution used was rat anti-HA-HRP 3F10 (Roche Applied Science), 1:2500-1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: Rat@HA (Roche 11 867 423 001, 1:500). Mouse@c-Myc9E10 (Santa Cruz sc-40 ...
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bioRxiv - Developmental Biology 2020Quote: ... Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, Mannheim, Germany) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Slides were incubated with rat anti-HA high-affinity (1:1000 dilution; Roche, clone 3F10) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used under heat fixation were anti-HA (rat monoclonal, 3F10; 1:500; Roche), anti-Canoe (rabbit 1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... The following reagents were used for visualization: DISCOVERY OmniMap anti-Rat HRP (Roche,760-4457) for CD31 or OmniMap anti-Rabbit HRP (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... For the detection of Pm8-HA anti-HA-HRP antibody (rat monoclonal, clone 3F10, Roche) was used at a dilution of 1:3000 ...
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bioRxiv - Biophysics 2023Quote: Lyophilized collagen containing telopeptides (Type I collagen from rat tail tendon, Roche cat. no. 11179179001) was purchased from Sigma Aldrich ...