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Citations for Roche :
51 - 100 of 229 citations for IL 13 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... rat polyclonal anti-Lid (1:500) [13] and mouse monoclonal anti-GFP (Roche #118144600001; 1:200). Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Library amplification (13 PCR cycles) was performed with KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems). Library size was determined by the Agilent 4200 Tapestation system ...
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bioRxiv - Genetics 2024Quote: ... The modified DNA was amplified with 13 cycles of PCR using Kapa U+ polymerase (Roche 07959052001) and indexed primers (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 200 U/ml of IL-2 (Roche) and 50 U/ml of penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 200 U/ml of IL-2 (Roche) and 50 U/ml of penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... human plasma fibronectin (Roche) was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
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bioRxiv - Synthetic Biology 2019Quote: The SPO-13-AM1 oligo still on beads was poly adenylated using TdT (Roche, Santa Clara, CA) and Duplase-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... All libraries and controls went through 13 PCR cycles using KAPA HiFi HotStart Ready Mix (KAPA Biosystems). PCR products were size-selected on a 1.8% agarose gel before loading on a Novaseq (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... XG1 cells were supplemented with IL-6 (Roche, 3ng/ml).
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bioRxiv - Microbiology 2023Quote: ... 1% P/S and 50 U/mL IL-2 (Roche), at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2023Quote: Cells were treated with DMSO or 0.5 μM dTAG-13 for 18 h and subsequently arrested at mitosis with 0.1 mg/ml colcemid (Roche) for 6 h (total 24 h) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were thawed on ice and disrupted in lysis buffer (100 mM Tris-HCl pH 7.4, 0.5% Triton X-100, 150mM NaCl, 13% w/v glycerol, protease inhibitor tablet, Roche) by bead beating (mini bead beater-16 ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Microbiology 2020Quote: ... and recombinant interleukin (IL)-2 (20U/ml; Hoffmann-La Roche, Italy). Cells without peptide stimulation and anti-CD3-stimulated (1μg/ml ...
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bioRxiv - Immunology 2021Quote: ... and 0.25 mg ml−1 Liberase (Roche Diagnostics, Chicago, IL, USA). The digested ears were passed through a 1 ml syringe to make single-cell suspensions ...
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bioRxiv - Immunology 2022Quote: Cells in cRPMI-10 with 20 U/ml IL-2 (Roche) were activated with Cell Stimulation Cocktail (Affymetrix eBioscience ...
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bioRxiv - Immunology 2023Quote: ... the media was supplemented with IL-2 [10 lU/mL] (Roche) and IL-15 [2 ng/ml] (BioLegend) ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 1% SDS, 0.1% Na deoxycholate, 1% Triton X-100, and 13 protease inhibitor cocktail [Roche]) was collected as nuclear proteins ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... with 250 μg/mL of Liberase TL (Roche Diagnostics, Chicago, IL, USA) for 60-90 mins in a shaker at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... CDA5 was generated by subcloning lmo0553 from the previously generated pET28a-lmo0553 vector[13] using Kapa HiFi polymerase (Kapa Biosystems) using primers 1 and 2 ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems, cat. KK8581) from 250 ng of total RNA ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems, Wilmington, MA, USA) followed by size selection from 1.5kb to 3.5kb with a BluePippin system (Sage Science ...
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bioRxiv - Immunology 2019Quote: ... assays were performed in the presence of 1000 U/mL IL-2 (Roche). Effector-target conjugates were incubated in 200 mL in round-bottom 96-well plates (Corning ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
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bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
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bioRxiv - Molecular Biology 2019Quote: ... 13 µL of 1:25 diluted cDNA was added to 16.25 µL KAPA 2x SYBR Fast Master Mix (KAPA Biosystems, Wilmington, MA) and 3.25 µL primer sets (Table 2.3 ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-Glutamine (R10) (Thermo 25030081) + 10 IU/mL IL-2 (Roche 11011456001) after CD3/CD28 stimulation ...
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bioRxiv - Immunology 2023Quote: ... a left lobe was digested for 1 hour at 37°C in RPMI containing 13 mg/mL DNase I (Roche, Randburg, South Africa) and 50 U/mL collagenase IV (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The fragmented samples were purified and concentrated to a volume of 13 µl using the SPRI method (Solid Phase Reversible Immobilization) with Kapa Pure Beads (Roche, cat. no. 07983298001). Elution was performed for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...