Labshake search
Citations for Roche :
201 - 250 of 466 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Linearized plasmids were then used as templates for in vitro transcription using either SP6 or T7 (Roche) using DIG labelled nucleotides (Roche) ...
-
bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
-
bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
-
bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
-
bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
-
bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
-
bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
-
bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
-
bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
-
bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
-
bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
-
bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA probes were generated via overnight incubation at 37°C using 20 U/µL T7 RNA polymerase (Roche) and digoxigenin (DIG)-labeled dNTPs (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled antisense or sense probes were synthesized using T7 or T3 RNA polymerase (Roche, 10588423 and 11031171001) and Dig RNA Labeling Mix (Roche ...
-
bioRxiv - Neuroscience 2020Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany). Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche ...
-
bioRxiv - Physiology 2020Quote: ... Synthesis of digoxigenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling Kit (SP6/T7; Roche).
-
bioRxiv - Neuroscience 2023Quote: ... and DIG-UTP labelled cRNA probes synthetized from linearized plasmids using either SP6 or T7 RNA polymerases (Roche) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Probes were transcribed with TranscriptAid T7 High Yield Transcription kit (Thermo, KO441) using DIG-Label UTP (Roche,11127073910).
-
bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... Digoxigenin (DIG)-labeled or fluorescein (FITC)-labeled riboprobes were synthesized with T7 or T3 or SP6 RNA polymerase (Roche) from the linearized plasmids with DIG or FITC RNA labeling mix (Roche) ...
-
bioRxiv - Molecular Biology 2022Quote: ... DIG-labelled RNA probes were synthesized using T7 or SP6 RNA polymerase and DIG RNA labeling Mix (Roche #11277073910), and free-nucleotides were removed using CENTRI-SEP Spin Columns (PRINCETON SEPARATIONS #CS-901).
-
bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche) after manufactures instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Antisense Digoxygenin-UTP riboprobes were synthesized using SP6 or T7 RNA polymerases and the DIG RNA Labeling kit (Roche).
-
bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
-
bioRxiv - Plant Biology 2022Quote: ... were produced with DIG-UTP by in vitro transcription using the Sp6 and T7 promoters according to the manufacturer’s protocol (RNA-labeling kit; Roche). The sections were treated as follows ...
-
bioRxiv - Genetics 2023Quote: ... The antisense probe for wdr31 was then transcribed with digoxigenin-labeled UTPs and T7 RNA polymerases (Roche, Basel, Switzerland). The stained embryos were dehydrated in glycerol and photographed with a Nikon SMZ1500 stereomicroscope (Nikon ...
-
bioRxiv - Developmental Biology 2023Quote: ... antisense probes were made using the appropriate T3/T7/SP6 RNA polymerases and the DIG labelling mix (Roche/Sigma), following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
-
bioRxiv - Developmental Biology 2023Quote: ... Sense and anti-sense riboprobes were generated using a digoxygenin RNA labelling kit and T3/T7 RNA polymerases (Roche). Images are representative of at least 3 embryos per condition.
-
bioRxiv - Plant Biology 2024Quote: ... 1 μg of the PCR product was used for RNA probe labeling with T7 RNA polymerase as per the DIG Northern Starter Kit’s Instruction Manual (Roche). Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche) ...
-
bioRxiv - Neuroscience 2023Quote: ... In vitro transcription was performed with PCR-amplified templates using T7 RNA polymerase (Hoffman La Roche AG, Basel, Switzerland) to synthesise antisense RNA probes (Supplementary Table 29) ...
-
bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
-
bioRxiv - Plant Biology 2021Quote: ... His-tagged AtTPL188 (wt and mutants) bacteria pellets were resuspended in buffer A with EDTA-free antiprotease (Roche). The soluble fractions recovered after sonication were passed through a Ni-sepharose (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... The His-tagged-TRF2TRFH protein was purified in presence of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) by using Protino ® Ni-TED 2000 Packed Columns (Macherey-Nagel ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
-
bioRxiv - Bioengineering 2024Quote: ... His-tagged rhFGFb was detected using a primary anti-HISx6 mouse polyclonal antibody (Roche Molecular Systems, Inc., USA) or anti-FGF polyclonal antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
-
bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
-
bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
-
bioRxiv - Immunology 2023Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
-
bioRxiv - Cell Biology 2020Quote: The RNA probes were transcribed with the T7 polymerase and labeled using the DIG RNA labeling kit (Roche Cat # 11175025910). After the labeling reaction was complete ...