Labshake search
Citations for Roche :
1 - 50 of 769 citations for IGFBP 7 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: HEK 293 cells were transfected with pcDNA4T/O-3FLAG::susF using FuGENE HD (Roche) transfection reagent according to manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfection of HEK-293 cells was performed with FuGene 6 transfection reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK 293 cells stably-expressing GFP-DFCP1 and RFP-ATG9 were generated by transfection of HEK 293 GFP-DFCP1 cell with RFP-ATG9 using X-tremeGENE 9 (Roche) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... These mutant viruses were made using HEK-293/17 cells that were transfected with 6 μg of viral DNA using Xtreme-GENE HP (Roche, Basel, Switzerland) Transfection reagent ...
-
bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
-
Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... His (Roche), GRASP55 (Proteintech) ...
-
bioRxiv - Biochemistry 2023Quote: ... or XtremeGene (HEK cells, Roche). Cells were fixed 48 h after transfection for 20 min with 3 % Formaldehyde/DBPS and permeabilized with 1 % Triton X-100/DPBS for 5 min at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... His-tagged rhFGFb was detected using a primary anti-HISx6 mouse polyclonal antibody (Roche Molecular Systems, Inc., USA) or anti-FGF polyclonal antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Cell Biology 2022Quote: ... and His-tag (11965085001, Roche) antibodies were used to detect the proteins.
-
bioRxiv - Biochemistry 2019Quote: ... anti-His-tag (11922416001; Roche), anti-vinculin (V4505 ...
-
bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
-
bioRxiv - Cell Biology 2019Quote: ... transfected into HEK-293T cells using X-tremeGENE9 (Roche) and single-cell sorted for GFP-positive cells using a Sony SH800 flow cytometer ...
-
bioRxiv - Biochemistry 2021Quote: ... cOmplete His-Tag Purification Resin (Roche) was added to the supernatant and samples were incubated overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Either His (Thermo) or GFP (Roche) or GST (Santa Cruz ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
-
bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
-
bioRxiv - Biophysics 2022Quote: ... the supernatants containing the His-tagged histones were combined with NiNTA resin (Complete His-Tag purification Resin, Roche) (1mL of Ni-NTA per 1L of bacteria ...
-
bioRxiv - Plant Biology 2024Quote: ... and 1/5000 anti-His (11667475001, Roche) for GFP and His-Tag detection ...
-
bioRxiv - Immunology 2024Quote: ... Adherent HEK 293T cells were transfected with Xtremegene HP reagent (Roche) according to manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-Fab were expressed with His-tag and purified using the cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
-
bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
-
bioRxiv - Biophysics 2020Quote: ... and amplified using KAPA Hi Fi polymerase (Roche) in 20 cycles of emulsion PCR35 (ePCR ...
-
bioRxiv - Biochemistry 2022Quote: ... purified by cOmplete His-Tag purification columns (Roche), and concentrated to 1 mg/mL using Amicon Ultra 10,000 NMWL (Merck) ...
-
bioRxiv - Microbiology 2020Quote: ... and cOmplete™ His-tag Purification Resin (Roche). Purity and glycosylation of recombinant proteins were examined by PNGase F (N-Zyme Scientifics ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cOmplete his-tag purification matrix from Roche is working equally well ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Complete His-Tag Purification Resin (Roche, Basel, Switzerland), or Amylose Resin (NEB ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 μg/ml insulin (Roche, #11376497001), and 15 mg/ml transferrin (Roche ...
-
bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
-
bioRxiv - Biochemistry 2020Quote: ... and incubated with cOmplete His-Tag purification resin (Roche) for 16 hours on a tube roller (Starlab ...
-
bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
-
bioRxiv - Plant Biology 2020Quote: ... 12 µl of complete His-Tag Purification Resin (Roche) was added and incubated for 15 min at 25°C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we used Kapa Hi-Fidelity Library Amplification Kits (Roche) in 20 µL reactions with 4µL Nextera Unique Dual Indexes Set A (Illumina) ...
-
bioRxiv - Cell Biology 2023Quote: ... or Complete His-Tag Purification Resin (Roche, Basel, Switzerland), that had been prewashed with respective lysis buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... 7 μg/ml transferrin (Cat.# 652202, Roche) and 150 units penicillin/streptomycin (Cat.#15140) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 7 μl proteinase inhibitor 7X (Roche, 04693159001) and 5 μl phosphatase inhibitor 10X (Roche ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.025% pronase (Roche, 7 U/mg)] in complete DMEM/F-12 medium in a spinner flask ...
-
bioRxiv - Cancer Biology 2021Quote: ... and TP53 (DO-7) (platform Ventana, Roche).
-
bioRxiv - Cell Biology 2021Quote: Clarified supernatants were rocked with Complete His-Tag resin (Roche), 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... and incubated with 4mL cOmplete His-Tag Purification Resin (Roche) for 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 ml of the cOmplete His-Tag purification resin (Roche) equilibrated with the Tris buffer 4 containing 25 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and purified using cOmplete His-Tag Purification Resin (Roche, Switzerland) and His-Bind Purification kit (Novagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... Addgene #12259 into HEK-293T cells using X-tremeGENE HP DNA transfection reagent (#06366236001; Roche). Lentiviral supernatants were applied to cells for 48h in the presence of polybrene (10 µg/ml ...
-
bioRxiv - Genomics 2022Quote: ... and amplified using KAPA Hi-Fi Hotstart PCR kit (Roche, KK2602). After one more step of DNA cleanup ...
-
bioRxiv - Microbiology 2020Quote: ... PCR was performed using KAPA Hi-Fi HotStart ReadyMix (KAPA Biosystems) and libraries were purified with AMPure XP magnetic beads (Beckman Coulter) ...
-
bioRxiv - Microbiology 2023Quote: ... The target protein was purified using His-Tag Purification Resin (Roche) according to the manufacturer instructions.
-
bioRxiv - Biochemistry 2020Quote: ... COS-7 cells were transfected using FuGENE6 (Roche) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... 7 μM KAPA Unique Dual-Indexed (UDI, Roche) adapters were ligated to the second strand synthesis product in the presence of a ligation master mix in a reaction that was performed at 20 °C for 15 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8 (clone SP57 at 1:7, Ventana- Roche), PD-1 (clone NAT105 ...
-
bioRxiv - Biophysics 2023Quote: ... and 7 U/mL DNase I (04536282001, Roche). The resuspended cells were rotated end-over-end for 30 min at 4 °C ...