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Citations for Roche :
301 - 350 of 5051 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Human genomic DNA of high molecular weight (Roche Diagnostics, Basel, Switzerland) were processed in a single replicate per DNA extraction protocol (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... Transcription was performed after manufacturer’s instructions except for the use of the DIG-labeling mixture (11277073910) from Roche. The RNA probes were diluted at 5 ng/µl in HybMix ...
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bioRxiv - Plant Biology 2020Quote: ... Gene expression was quantified using real-time reverse transcription PCR (qRT-PCR) with SYBR Green and ROX (Roche). Gene quantification was performed in a Step One Plus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was linearized and an in vitro transcription reaction with DIG labeling mix (Roche, cat. no. 1277073) was performed as previously described [18] ...
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bioRxiv - Physiology 2024Quote: Digoxigenin (DIG)-labeled cRNA probes were generated by in vitro transcription using DIG RNA Labeling Mix (Roche Diagnostics), T7 RNA polymerase (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2022Quote: For antibody incubation 5 µl of the DigiWest Bead mixes were added to 50 µl assay buffer (Blocking Reagent for ELISA (Roche, Rotkreuz, Switzerland) supplemented with 0.2% milk powder ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription of antisense and sense probes was performed with a DIG-RNA Labelling Mix (Roche, Mannheim, Germany) using either T7 or T3 polymerase.
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Half of the reverse transcription reaction was used for amplification with a KAP real-time PCR mixture (KAPA Biosystems) using the Illumina Truseq small RNA library amplification kit primers ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 ng of cDNA was used for quantitative reverse transcription PCR with the FastStart SYBR Green Master (Roche) and the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... In vitro transcription was performed with PCR-amplified templates using T7 RNA polymerase (Hoffman La Roche AG, Basel, Switzerland) to synthesise antisense RNA probes (Supplementary Table 29) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human samples using TriPure Isolation Reagent (Roche). RNA integrity (RIN ...
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bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... eosin and antibodies for human keratins AE1/AE3 (760-2135; Roche diagnostics). Metastasis quantification was reached through a review of 17 digitalized slides ...
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... thermophila intron were obtained by in vitro transcription of the corresponding linearized plasmids with 20 U of T3 bacteriophage RNA polymerase (Roche) in 20-μl reactions containing 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were synthesized from cDNA templates via T3 RNA polymerase mediated strand-specific RNA transcription using DIG/FL RNA labelling mix (Roche). Hybridised ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were hybridized with antisense RNA probe for IR84a (nucleotides 82-780) or IR8a (nucleotides 4-529) sequence labelled using in vitro transcription with digoxigenin-11-UTP (Roche). The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
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bioRxiv - Physiology 2021Quote: ... mRNA expression levels were measured after reverse transcription by quantitative real-time PCR (qRT-PCR) with FastStart SYBR Green master mix (Roche) using a LC480 instrument (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative reverse transcription PCR (RT-PCR) was performed with 10 ng of cDNA in a Light Cycler® Instrument (Roche) using LightCycler® 480 probes master mix (4887301001 ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes (sense and antisense) were generated by transcription in vitro using SP6 or T7 RNA polymerases (Roche) as previously described (Cruz et al. ...
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bioRxiv - Developmental Biology 2022Quote: Antisense RNA probes for in situ hybridization were prepared by in vitro transcription using Digoxigenin- or Dinitrophenyl-conjugated UTP (DIG, Roche 11277073910 ...
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bioRxiv - Cancer Biology 2019Quote: Reverse transcription (RT) reactions were carried out using 1 μg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Roche Diagnostics ...
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bioRxiv - Developmental Biology 2019Quote: ... where the reverse primer contains a T7 promoter sequence (in caps) for in vitro transcription using DIG labeling mixtures (Roche) and T7 RNA polymerase:
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The cDNA templates were then used for Sybr green real-time PCR to determine the transcription level of viral genes with a Light Cycler 480 system (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... 500 ng DNA was subjected to in vitro transcription with T3 polymerase and DIG- or FITC-labeled ribonucleotides (All Roche) for 2 hours at 37°C ...
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bioRxiv - Physiology 2020Quote: ... mRNA expression was measured after reverse transcription by quantitative real-time PCR (qRT-PCR) with FastStart SYBR Green master mix (Roche) using a LightCycler Nano or LC480 instruments (Roche) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Templates for riboprobes were designed by attaching a T7 promoter through PCR and performing a DIG labelled transcription reaction (Roche). The same H ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of the viral RNA and amplification of the cDNA was conducted in a LightCycler 480 System (Roche, Germany) using the following protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reverse transcription and QPCR were performed using Transcriptor reverse transcriptase and Sybr Green Master Mix (Roche Diagnostics Corp., Indianapolis, IN). The following genes were examined ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time reverse transcription PCR assays were conducted in 96-well plates using the LightCycler 480 Instrument (Roche, Wilmington, MA). Levels of mRNA expression were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Physiology 2023Quote: ... Gene expression was measured after reverse transcription by quantitative real-time PCR (qRT-PCR) with FastStart SYBR Green master mix (Roche) using a LightCycler Nano or LC480 instruments (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription polymerase chain reaction (RT–PCR) was performed on the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg linearized plasmid was used for In Vitro transcription to make DIG labelled RNA probe (DIG RNA Labeling Mix-11277073910 Roche). Whole mount RNA in situ hybridization was performed by following the standard zebrafish protocol (http://zfin.org/ZFIN/Methods/ThisseProtocol).
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product was then used as a template for riboprobe synthesis by in vitro transcription using SP6 polymerase and digoxigenin-labelled dUTPs (Roche). Each riboprobe was tested a minimum of two times ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using qScript (Quanta Biosciences) followed by real-time PCR using KAPA SYBR FAST ROX Low (Roche) on the ViiA 7 Real-Time PCR instrument (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... Resulting amplicons were used as templates for the synthesis of either digoxigenin (DIG) or fluorescein (FITC)-labelled anti-sense probes by in vitro transcription using T7 or T3 polymerases (Roche) and DIG or FITC RNA labelling mixes (Roche).
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bioRxiv - Physiology 2024Quote: ... Gene expression was measured by quantitative reverse transcription PCR (qPCR) using LightCycler 480 SYBR Green I Master (50-720-3180 Roche). Quantitative polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay.
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bioRxiv - Cancer Biology 2021Quote: ... Pooled sample libraries were hybridized with SeqCap EZ Human Exome v3.0 probes (Roche) for WES ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
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bioRxiv - Immunology 2024Quote: Human monocytes were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...