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Citations for Roche :
551 - 600 of 6321 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
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bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg nuclear lysate was adjusted to 500 uL in Protein Incubation Buffer (PIB+++; 150mM NaCl, 50 mM Tris pH 8.0, cOmplete protease inhibitor (Roche, cat 04693159001), 0.25% NP-40 and 1 mM DTT) ...
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bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
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bioRxiv - Molecular Biology 2021Quote: Serum albumin measurements were determined using a commercial serum albumin kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on Cobas Mira analyser (Roche Diagnostics Ltd ...
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were prepared from HCC1428 cells with control or E2-E10 siRNA knockdown using modified Tissue Protein Extraction Reagent (TPER) (Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The protein lysates were then diluted into 0.5 mg/ml of total protein in SDS sample buffer and denatured on the same day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Microbiology 2022Quote: ... The gene encoding this protein was amplified from genomic DNA of the SECphi4 phage using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers AS330+AS331 (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: Total protein cell extracts were obtained by lysing the cells with RIPA buffer supplemented with 1x protease inhibitors (PI) (Roche, Milan, Italy). Proteins were quantified using the BCA method (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were prepared using T-PER (Pierce Chemical Co., IL) in the presence of protease inhibitors (Roche Applied Science, Barcelona, Spain), and subjected to Western blot analyses with anti-rat anti-ABCA1 antiserum (kindly donated by Dr ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates of 20 μg protein were separated in a SDS-12% polyacrylamide gel (PAGE) followed by transferring proteins to a polyvinylidene difluoride (PVDF) membrane (Roche, catalog 3110040). After blocking in 5% milk (in 1xphosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Molecular Biology 2021Quote: ... HCXII-0155) was incubated with neutrophils that were pre-treated with vehicle or DNase I (10U/ml, Dornase alpha, Roche, Germany) for 1h ...
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bioRxiv - Immunology 2024Quote: ... the cells were quickly transferred into 10 ml of pre-warmed RPMI supplemented with 10% FBS and 100µg of Pulmozyme (dornase alpha, Roche, Basel, Switzerland) for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect electroporation-competent cells ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg of proteins from the microsomal membrane pellet (P13) were incubated with 30 μg/ml of proteinase K (Roche Diagnostics, Mannheim, Germany) for 30 min on ice ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cell Biology 2023Quote: ... were used to remove salts from purified protein and to exchange to freezing buffer (20mM HEPES, 100mM NaCl, 0.5mM EDTA, TCEP 0.5mM and Roche cOmplete™ Protease Inhibitor Cocktail), using the gravity protocol ...