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Citations for Roche :
351 - 400 of 431 citations for Human MMP24 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: We amplified the Tug1 cDNA sequence with primers (see Sequences and Primers below) having KpnI and NotI restriction enzyme overhangs from the pTRE2-Tug1 vector plasmid using Q5 polymerase (Roche) and under following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Handles were PCR-generated from the plasmid pSP73-JY0 (Fili et al., 2010) using appropriate oligonucleotides (Supplementary Table S1) and Bio-dUTP or Dig-dUTP (Roche), followed by restriction with PsiI or KpnI ...
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bioRxiv - Genomics 2019Quote: ... the cells were transfected with 10 μg of the plasmid libraries (HSS, ORI, and pGL4) using X-tremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections and antibody staining were performed as follows: two micrograms plasmid DNA was transfected using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 ug of shRNA/cDNA and 1 ug of viral packaging plasmids (250ng pMD2.G and 750ng psPAX2) using 6 ul of Xtreme Gene 9 transfection reagent (Roche). After 24 hr ...
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bioRxiv - Genetics 2021Quote: Overexpression plasmids were respectively transfected into U251-APP cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). Cells grown in 6-well plate were transfected by overexpression plasmid of the target gene or its corresponding control plasmid (each 2 μg ...
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bioRxiv - Biochemistry 2021Quote: For transient transfection cells were seeded and after 18 h cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche), according to the manufacturer’s protocol (for 12-well plate format ...
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bioRxiv - Biochemistry 2021Quote: ... and pPB-tetIRB-tetON-BSD plasmid bearing CLPTM1L-mEGFP or pPB-tetON-BSD-tightFF plasmid bearing CLPTM1L-FLAG-6His using X-tremeGENE 9 DNA transfection reagent (Roche), and incubated at 37 °C for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
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bioRxiv - Microbiology 2021Quote: ... and transfected with plasmid pIIIH5red-SARS-2-S DNA using X-tremeGENE HP DNA Transfection Reagent (Roche Diagnostics, Penzberg, Germany) according to the manual ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were cloned in pBlueScript KS+ vector and digoxigenin-labelled probes were generated from linearized plasmids using RNA-labeling mix (Roche). Whole-mount chromogenic in situ hybridization was performed as described previously (Marchal 2009 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then transiently transfected with MaMTH bait (MMD or MBOAT7) and prey (MMD, MBOAT7 or PEX7 negative control) plasmids using X-tremeGENE9 DNA transfection reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche, 6366236001), at a ratio of 400 ng DNA to 40 µL 37 °C serum-free DMEM to 1.6 µL reagent per dish being transfected ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... grown for 24 hours and transfected with 8 μg of plasmids and 8 μl of X-tremeGENE HP DNA Transfection Reagent (Roche). The cells were grown for 48 hours post-transfection and collected for downstream analyses.
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bioRxiv - Developmental Biology 2023Quote: ... Purified plasmids were used to prepare digoxigenin-labelled sense and anti-sense riboprobes using T7 and SP6 RNA polymerases (Roche). Probes were purified using ethanol precipitation and resuspended in 1:1 volume of DEPC-treated water:formamide.
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: Parental and Haspin knockout cells stably expressing GFP-TAF5 were obtained by cotransfection with pcDNA5/FRT/TO/GFP-TAF5 (Antonova et al., 2018) and a pPGKpuro resistance plasmid (in a 9:1 ratio) using Xtreme Gene 9 (Roche). After 48 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg linearized plasmid was used for In Vitro transcription to make DIG labelled RNA probe (DIG RNA Labeling Mix-11277073910 Roche). Whole mount RNA in situ hybridization was performed by following the standard zebrafish protocol (http://zfin.org/ZFIN/Methods/ThisseProtocol).
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bioRxiv - Developmental Biology 2022Quote: ... Plasmids were linearized and probes were prepared as described in Wilkinson & Nieto (1993) using digoxigenin-conjugated nucleotides (Roche, Basel, Switzerland). Sense probes were used to confirm specificity of the antisense probes ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells were transfected with PB-Tet-LAP-MAP3K1 and PB-Transposase plasmid (kindly provided by N. Dimitrova) using X-tremeGENE9 reagent (Roche) and after 48 hours cells were selected with G418 ...
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bioRxiv - Microbiology 2023Quote: ... a 2.3 kb-homologous DNA fragment was cloned into a unique SpeI site of a non-replicative plasmid (pIJ10700) using T4 DNA ligase (Roche). The fragment was amplified using Q5® DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T and MEF cells (except for p62 KO MEF cells) were transfected with plasmids using X-tremeGENE 9 (Roche) as described as previously (41–43) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was stopped by the addition of 500 ng of plasmid (size > 10 kb) and 0.1 pmol of ATP-γ-S tetralithium salt (10102342001-Roche, Sigma) followed by incubation for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
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bioRxiv - Biochemistry 2022Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... transient transfection was performed with a total of 1 µg of plasmids (EGFRmCitrine, PTBmCherry and cCblBF P at ratio 4:3:4 by mass) using FUGENE6 (Roche Diagnostics) transfection reagent and Opti-MEM (Gibco - Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml, Roche (Nutley, NJ)) ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Biochemistry 2021Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells transfected with a combination of RCAS plasmids (RCAS PDGFB-HA, RCAS shp53-RFP) using the FuGENE 6 transfection kit (Roche, 11814443001) according to the manufacturer’s protocol and as previously described 86 ...
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmids were transfected into cultured cells susceptible to the original viruses using X-tremeGENE™ HP DNA transfection reagent (Roche). For BmNPV ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mg of plasmid DNA was transfected into HMEC P5 was transduced into Phoenix packaging cells using Fugene (Roche, Basel, Switzerland). Viral supernatant was harvested 48 h after transfection ...
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bioRxiv - Biochemistry 2022Quote: Plasmids were constructed using standard molecular biology techniques of PCR and Golden Gate assembly with Kapa HiFi DNA Polymerase (KAPA Biosystems), restriction enzymes (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... IFNγ-R2-eGFP plasmid was transfected in HeLa or Caco-2 cells with X-tremeGENE HP DNA transfection reagent (Roche, 6366236001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: A DIG-labeled antisense probe against goosecoid (gsc) was generated by in vitro transcription from a linearized plasmid using 10x Transcription buffer (Roche, #11465384001), 10x DIG RNA labelling mix (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Microbiology 2023Quote: ... qPCR standard curves were determined using linearized plasmids as DNA templates and run on a Light Cycler 480 system (Roche Diagnostics) in duplicates ...
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bioRxiv - Bioengineering 2022Quote: siRNAs and plasmids were transfected using DharmaFECT 1 transfection reagent (Dharmacon T-2001) and X-tremeGENE9 DNA transfection reagent (Roche 06365809001) respectively ...
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bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
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bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...