Labshake search
Citations for Roche :
201 - 250 of 431 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... plasmid DNA transfections were performed using the X-tremeGENE HP DNA transfection reagent (#06366236001, Roche) in a 2:1 DNA/transfection reagent ratio according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
-
bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
-
bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
-
bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
-
bioRxiv - Immunology 2021Quote: ... also available at addgene (plasmid 15912) (http://www.addgene.org/15912/) using the Roche Rapid ligation kit (Roche). The correct insert orientation was confirmed by NheI and SmadI (NEB ...
-
bioRxiv - Immunology 2021Quote: ... Viral plasmids were transduced in HEK-293T cells using X-tremeGENE HP DNA transfection reagent (Roche). After the addition of X-tremeGENE HP to the viral plasmids the solution was mixed in 100 µL of DMEM medium without serum or antibiotics ...
-
bioRxiv - Microbiology 2020Quote: ... the DNA library of pDJB-3 plasmid prepared by KAPA Hyper Prep Kit (Roche, Basel, Switzerland) gave a pool of 150 bp paired-end reads that are destined to be assembled into a contig by the SPAdes Genome Assembler (version 3.11.0) ...
-
bioRxiv - Cancer Biology 2020Quote: Transient transfection of plasmids for overexpression purposes was performed using X-tremeGENE™ HP (Roche Diagnostics) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 µg of each plasmid was combined with Fugene transfection reagent (Roche Applied Science, Mannheim, Germany) at a ratio of 5 µl of transfection reagent to 1 µg DNA in 500 µl of MEM and incubated at room temperature for 30 minutes prior to addition to a 10 cm dish of HEK293 cells at 50% confluence ...
-
bioRxiv - Genetics 2020Quote: ... BAC DNA from clones was isolated using a High Pure Plasmid Isolation Kit (Roche Applied Science) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: Separate PLAT-E cultures were transfected with 9µg of each pMX plasmid using FuGENE 6 (Roche) for retroviral production ...
-
bioRxiv - Genomics 2021Quote: ... The p415-CYC1-HIS3 plasmid was linearized by inverse PCR using KAPA HiFi polymerase (Kapa Biosystems) with primers F-p415-His and R-p415-HIS (oligonucleotide sequences in Supplementary Table 1) ...
-
bioRxiv - Microbiology 2020Quote: ... and were transfected with indicated plasmids by the X-tremeGENE HP DNA Transfection Reagent (Roche, USA) when the cell confluence was up to 90% ...
-
bioRxiv - Molecular Biology 2022Quote: ... structure in the 5’UTR and 70 ng of firefly luciferase (FLuc)-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: ... or a HP50 hairpin structure in the 5’UTR and 2 ng of FLuc-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting plasmids were used for RNA probe synthesis with digoxigenin (DIG) labeling mix (Roche, #11277073910) or fluorescein (FITC ...
-
bioRxiv - Microbiology 2023Quote: ... UL16 and UL21 expression plasmids using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2.5 ug plasmid DNA per well using DOTAP Liposomal Transfection Reagent (Roche) following manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: The BSEP promoter plasmid based on pGL3-basic (BSEPprom-Luc) was a kind gift from Roche. The human SHP promoter (bases -572 to +10 ...
-
bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
-
bioRxiv - Microbiology 2024Quote: ... plasmids were transfected into HeLa cells using X-tremeGENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
-
bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
-
bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... freshly resected human samples were cut into small fragments and digested with 0.1 mg/ml Liberase TL (Roche) and 0.1 mg/ml DNase (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
-
bioRxiv - Neuroscience 2020Quote: ... human vimentin immunohistochemical staining of the paraffin sections was performed by using the Ventana Discovery XT instrument (Roche) with Ventana DAB Map detection Kit (760-124 ...
-
bioRxiv - Genetics 2020Quote: ... we combined 800 ng of plasmid library with 25 µL 2x Kapa library amplification mix (Roche, #KK2702), 1 µL forward primer (10 µM ...
-
bioRxiv - Systems Biology 2020Quote: ... The cloning of the gRNAs sequences was performed via full plasmid PCR amplification with the KAPA (Roche) polymerase using 60-mer oligonucleotides (Eurofins Genomics) ...
-
bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... HeLa WT cells were transfected with the guide containing plasmid using the transfection reagent XtremeGENE HP (Roche) according to manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... the plasmid was used for in vitro RNA transcription in the presence of DIG labelling mix (Roche) and RNA Polymerase (see Table S4 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The plasmid for the probes were linearized and transcribed in vitro using DIG RNA labeling mix (Roche; supplementary material Table S1 for plasmid templates for RNA synthesis).
-
bioRxiv - Developmental Biology 2022Quote: HEK293T at 60-80% confluence were transfected with plasmids amplified from an overexpressing lentiviral library together with 0.975µg CMVΔR8.74 and 0.525µg VGV.G packaging plasmids using X-tremeGENE™ HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Transient transfection was achieved by transfecting cells with plasmid DNAs using XtremeGene HP DNA Transfection reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... TCR or CAR plasmid pools were used as templates for PCR amplification (KAPA HiFi HotStart ReadyMix, Roche) to generate double-stranded DNA templates including truncated Cas9 target sequences (Nguyen et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... Linearized plasmids were then used as templates for in vitro transcription using either SP6 or T7 (Roche) using DIG labelled nucleotides (Roche) ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche). For silencing experiments ...
-
bioRxiv - Developmental Biology 2024Quote: ... All riboprobes were generated from linearized plasmids and synthesized using the DIG RNA Labelling Kit (Roche, 11175025910).
-
bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
-
bioRxiv - Neuroscience 2019Quote: ... barcoded and enriched using the NimbleGen SeqCap EZ Human Exome Library v2.0 enrichment kit (Roche NimbleGen, Madison, WI, USA). Purified and quantified library pool was subsequently sequenced on an Illumina HiSeq 2000 sequencing instrument (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... and human acute promyelocytic leukemia cell line NB4 (ATCC) were washed once with the digestion buffer for neuraminidase (Roche), the cells were centrifuged at 2000 rpm for 5 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled RNA probes for human NORAD were synthesized by in vitro transcription using a DIG-labeling mix (Roche). Primers used for amplification of the DNA template for each probe are provided in Supplementary File 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 ng of nick translated probe per coverslip was combined with 12 μg of human Cot-1 DNA (Roche), 10 μg salmon sperm ssDNA (Sigma-Aldrich ...