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Citations for Roche :
501 - 550 of 6347 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 3 U/ml dispase II (Roche Diagnostics, 4942078001), and 10 mM CaCl2 for 30 min at 37 °C with occasional shaking ...
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bioRxiv - Biochemistry 2019Quote: ... in low salt solution (0.5X PBS; 0.1 mM EGTA; protease inhibitor cocktail (Roche cat #05056489001). N-ChIP chromatin bound to Protein G Sepharose beads (GE Healthcare cat #17-0618-02 ...
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bioRxiv - Immunology 2020Quote: ... and diluted in H2O for qPCR using ROX low SYBR FAST 2X mastermix (KAPA/Roche) and QuantStudio6 qPCR machine (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: ... in low salt solution (0.5x PBS; 0.1 mM EGTA; protease inhibitor cocktail (Roche cat #05056489001). nChIP chromatin bound to Protein G Sepharose beads (GE Healthcare cat #17- 0618-02 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked using 10% skimmed milk in PBS containing 0.1% Tween 20 (PBST) and then incubated with rat anti-HA high affinity (1:1,000; Roche) and rabbit anti-T ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
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bioRxiv - Biophysics 2021Quote: ... The cleavage mixture was then dialysed back into the lysis buffer and incubated with Nickel affinity gel (Roche) for one hour to separate the cleaved protein from the TEV protease and tag ...
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bioRxiv - Biochemistry 2022Quote: ... the AAC2 variants were imported into Tom40-HA mitochondria followed by affinity purification via anti-HA beads (Roche) (18) ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were incubated for three hours in anti-HA High Affinity antibody from rat IgG (clone 3F10; Roche), used at 1:1200 dilution in TBS Tween 20 (0.1% ...
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bioRxiv - Cell Biology 2021Quote: ... adapter-ligated libraries were prepared with the KAPA Hyper Prep Kit and sequencing libraries were constructed using SeqCap EZ Human Exome Library v3.0 (Roche, Basel, Switzerland). Cluster generation was performed with the HiSeq PE Cluster Kit v4 (Illumina ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... blots were hybridized with digoxigenin (DIG)-labeled optrA-specific probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Sciences, Germany) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
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bioRxiv - Microbiology 2019Quote: ... Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Purified DNA from mouse fecal pellets or sorted cells was subjected to 16S variable region 4 PCR amplification using barcoded 515F and 806R primers (47) and the KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), with the following cycling conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Neuroscience 2021Quote: ... Briefly, ganglia from adult Aplysia were treated with Dispase® II (10 units/ml) (neutral protease, grade II) (Roche, Indianapolis, IL) at 35°C for ∼3 h and then desheathed ...
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bioRxiv - Immunology 2023Quote: ... small piece of one lobe (1mg/ml Collagenase I Gibco 17100-017, 1mg/ml Collagenase II Wothington LS004176, 5mg/ml Dispase II Sigma D4693 and 7.5ug/ml DNAse I Roche 11284932001, 30min) Lung ...
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bioRxiv - Biochemistry 2019Quote: ... Blocking reagent for ELISA (BRE) and cOmplete protease inhibitor tablets were from Roche (Basel, Switzerland). Phosphate buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: ... 100uL of the clarified supernatant was used in the anti-BrdU DNA fragmentation ELISA (Roche). Each condition was tested in technical triplicates ...
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bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
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bioRxiv - Genomics 2023Quote: ... Converted cDNA is quantified using 2x KAPA SYBR Fast Master Mix ROX Low (KAPA Biosystems, KK4620), with primers against upstream RHOXF2B TSS ...
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bioRxiv - Bioengineering 2019Quote: ... For molecular cloning 3 µg of RNA were reverse transcribed for 30 min at 50°C using an oligo(dT) primer and the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) followed by 5 min enzyme inactivation at 85°C according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... the blot was washed at 55°C and probe detection was performed according to the manufacturers’ protocol using the DIG luminescent detection kit (Roche). The intensity of individual bands was measured with respect to the background and integrated density value (IDV ...
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bioRxiv - Cell Biology 2022Quote: mRNA was purified from ∼200 ng of total RNA with oligo-dT beads and sheared by incubation at 94°C in the presence of Mg2+ with the KAPA mRNA HyperPrep kit (Roche). Following first-strand synthesis with random primers ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Telomere dot blot was performed by 3h hybridization at 42°C with DIG-labelled telomere probe as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). Control Input was 200 ng genomic DNA from APCs ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10% FCS (P3 and P9) and treated with 0.05 mg/ml Liberase TH (Roche) and 0.2 mg/ml DNAse (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems), according to the QuikChange site-directed mutagenesis manual ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were removed by centrifugation (twice at 1,000 xg) and then pre-cleared lysates were supplemented with anti-HA high affinity monoclonal antibody (clone 3F10, Roche) at 2 µg/ml final concentration along with 20 µl Protein G Sepharose beads and incubated at 4° C for 4 hours on a rotating wheel ...
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bioRxiv - Cell Biology 2019Quote: ... and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10; 1:50 dilution, Roche). The sections were rinsed in PBS before incubation for 30 minutes with secondary Immunotech biotinylated antibody and 45 minutes with the Streptavidine-Peroxidase Complex (universal HRP immunostaining ...
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bioRxiv - Plant Biology 2021Quote: ... SMXL5-3xHA and 6xMyc-OBE3 bands were detected by antibodies Anti-HA-Peroxidase High Affinity (3F10) (Roche; Basel, Switzerland) or c-Myc Antibody (9E10 ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Microbiology 2023Quote: ... we amplified the V3-V4 hypervariable region of the 16S rRNA gene via PCR with Hifi Hotstart Readymix (Kapa Biosystems; Boston, MA), forward primer 5’-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3’ ...
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bioRxiv - Neuroscience 2023Quote: Whole brains or dentate gyrus regions (isolated from the brain slices) were lysed in 2% sodium dodecyl sulfonate (SDS) with protease and phosphatase inhibitors (Roche Applied Sciences) and manually homogenized ...
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bioRxiv - Genomics 2020Quote: ... and 10 U/ml Dispase II (Roche, Basel, Switzerland), followed by manual dissociation to generate cell suspensions ...
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bioRxiv - Microbiology 2020Quote: ... grade II from bovine pancreas (Roche, Cat. No. 10104159001) was resuspended in a storage buffer (10mM Tris-Cl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... and ran on Light cycler 480 II instrument (Roche). The reactions were carried out in technical triplicates ...
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bioRxiv - Cell Biology 2022Quote: qPCR was performed using LightCycler® 480 II (Roche). The reaction contained 5μl of a 1/10 dilution of the cDNA obtained after RT (25ng of cDNA) ...
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bioRxiv - Genomics 2020Quote: ... Reactions were run on the LightCycler 480 II (Roche), with an initial incubation step of 95°C ...
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bioRxiv - Bioengineering 2020Quote: ... with dispase II (1.2 U/mL final, from Roche) and CaCl2 (2.5 mM ...
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bioRxiv - Physiology 2020Quote: ... We ran assays on a LightCycler 480 II (Roche) machine using the SsoAdvanced Universal SYBR Green Super mix (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... using a LightCycler® 480 Instrument II (Roche, 05015243001). The results were calculated by ΔΔCt method using human HPRT (hHPRT ...