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Citations for Roche :
151 - 200 of 431 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a sgRNA were transfected into HeLa cells with X-tremeGENE8 (Roche). Single GFP-positive cells were sorted by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA probes complementary to human METTL7B cDNA (NM_152637.2) were labeled with digoxigenin-UTP (Roche). After acetylation ...
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bioRxiv - Genetics 2022Quote: ... Probes were then mixed with 20μg of COT Human DNA (Roche, 11 581 074 001) and 79μg of Salmon sperm DNA (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Human periocular skin was digested in 2.4 U/ml dispase type II (Roche, Basel, Switzerland) at 4 °C for 12 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and ethanol precipitated with human Cot-1 DNA (Roche, Germany) and herring sperm DNA (Sigma Aldrich) ...
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bioRxiv - Immunology 2020Quote: Data on miRNA expression were obtained from the FANTOM5 dataset (https://fantom.gsc.riken.jp/5/suppl/De_Rie_et_al_2017/vis_viewer/#/human) and from (18) (Cohort Roche, GEO accession: GSE28492). The FANTOM5 dataset was downloaded and analyzed using Qlucore Omics Explorer software ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Cancer Biology 2023Quote: ... and retrotranscribed cDNA quantified using the Universal Human Probe Roche library (Roche-Diagnostics, Barcelona, Spain). Assays were made in triplicate and normalized to TBP expression (ΔΔCT method) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfections were carried out using X-tremeGENE 9 DNA transfection reagent (Roche Diagnostics) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... directly from genomic DNA or cloned plasmid templates to incorporate DIG-labeled dUTP (Roche) using the primers described in Extended Data Table 5 ...
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bioRxiv - Microbiology 2019Quote: ... large-scale insertions and plasmid sequences were assembled de novo using Newbler (Roche, Inc.), and integrated into the genome where connectivity was supported by evidence from paired reads ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed after cells had reached ~90% confluency using Lipofectamine reagent (Roche), as recommended by the manufacturer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid transfections were carried out with X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were replated in 96-well plates the next day at 2 × 104 cells/well density and transfected with poly I:C (700 ng in 110 μl volume ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche) as per the manufacturer’s protocol using a total vector amount of 2 µg (6-well ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were incubated for 20 min with a mix of XtremeGENE9 transfection reagent (Roche) and OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were transfected into HEK293T cells using X-tremeGENE 9 (Roche # XTG9-RO) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We re-circularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with 10 µg of each plasmid DNA using XtremeGene HP (Roche) transfection reagent ...
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bioRxiv - Genomics 2019Quote: The gDNA libraries were prepared using a SeqCap EZ Human Exome Library v2.0 (Roche, Basel, Switzerland). Sequencing was performed with 100-bp paired-end reads by the HiSeq2000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790-4325), human PR rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids were transfected in HeLa and YFP-Parkin HeLa using Xtreme Gene 9 (Roche) according to manufacturer’s directions ...
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bioRxiv - Cell Biology 2020Quote: ... the above-mentioned SEPT9 plasmids were amplified with KAPA HiFi HotStart DNA polymerase (KAPA BIOSYSTEMS) using the primers 5’-GGAGCGCATCCCCAAGGGCATCGAGATCAAGTCC-3’ and 5’-GGACTTGATCTCGATGCCCTTGGGGATGCGCTCC-3’ ...
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bioRxiv - Microbiology 2019Quote: ... 500 ng of viral RNA or plasmid DNA was treated with DNase I (10U, Roche), XrnI+RppH (1U XrnI + 5U RppH ...
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bioRxiv - Cancer Biology 2020Quote: Transfection of plasmids for overexpression purposes was performed using X-tremeGENE™ HP (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... BAC DNA from clones was isolated using a High Pure Plasmid Isolation Kit from Roche Applied Science (PN ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were linearised and probes synthesised according to DIG RNA Labeling Kit (SP6/T7) (Roche). Zebrafish larvae were fixed in 4% paraformaldehyde solution (PFA ...
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bioRxiv - Immunology 2020Quote: ... Point mutations within Aire plasmids were generated by using KAPA HiFi Hot start (Kapa Biosystems) or Phusion® High Fidelity (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 µg sgRNA plasmid using X-tremeGENE™ 9 DNA transfection reagent (Roche 06365809001). One day after transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting plasmids were used for RNA probe synthesis with digoxigenin (DIG) labeling mix (Roche), and T7 or T3 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmid DNA was transfected to HEK293T cells using X-tremeGENE HP DNA transfection Reagent (Roche). Transfected cells were harvested at 48◻hours after transfection for further analysis.
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bioRxiv - Genetics 2020Quote: ... Digoxigenin-labeled probes were generated from linear plasmid DNA using DIG RNA Labeling Mix (Roche) and hybridized probes were detected with anti-digoxigenin antibody (Anti-Digoxigenin-AP ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were introduced into HEK293T cells using the FuGENE 6 transfection reagent (Roche Applied Science). Cells were selected with puromycin (1 μg/ml) ...
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bioRxiv - Genetics 2020Quote: ... The plasmid was linearized and used for in vitro transcription (Roche Dignostics, Indianapolis, IN, USA) to label RNA probes with digoxigenin-UTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μg of plasmid and 4 μl of X-tremeGENE HP DNA Transfection Reagent (Roche) were used per well ...
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bioRxiv - Cell Biology 2023Quote: ... Both plasmids were transfected into HeLa cells using the X-tremeGENE9 DNA Transfection Reagent (Roche) and selected in medium containing G418 (1 mg/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Biochemistry 2024Quote: ... yielding the plasmid constructs pXOON-r5-HT3A and pXOON-gp5-HT3A (Roche Diagnostics, Mannheim, Germany). Point mutations were generated by site-directed mutagenesis using the QuickChange mutagenesis kit (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmids were transiently transfected into HeLa cells using Fugene HD transfection reagent (Roche, Germany) for 48 hours to express the corresponding E2F-EGFP fusion proteins ...