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Citations for Roche :
451 - 500 of 10000+ citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl of each forward and reverse primer (5 uM) and 10 μl SYBR green (Roche, 4707516001) were used for qPCR (30 sec at 98 °C and 19 cycles of 10 sec at 98 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).
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bioRxiv - Molecular Biology 2022Quote: Radioimmunoprecipitation Assay (RIPA) buffer and cOmplete mini-proteinase inhibitors (1 tablet per 10 ml extraction solution, Roche) was used to homogenize striatal tissue and cells ...
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bioRxiv - Physiology 2023Quote: ... Both kidneys were incubated in 10 ml enzymatic solution (1 mg/ml collagenase A (Roche Diagnostics, Germany), 2.5 mg/ml trypsin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 400 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.4% [vol/vol] Nonidet P40, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free (MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: • PCR #1: 10 μl reactions were prepared for each sample with KAPA HiFi HotStart ReadyMix (Roche, KK2602) containing 1 μl cDNA template and 1.5 μl of 10 μM PCR1 primer mix (U6 outer ...
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2023Quote: ... or Nunc Glass Base Dishes (Thermo Fischer Scientific) coated with sterile 10 µg ml−1 fibronectin (Roche). Supplier and identifier for all reagents are listed in Table 4 ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Biochemistry 2024Quote: ... 10% (v/v) glycerol and 1× Halt or 1x cOmpleteTM EDTA-free protease inhibitor cocktail (Roche #11873580001). Lysates were sonicated with a probe sonicator and cleared via centrifugation at 15,000x g for 5 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from 1 μg samples of total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) kit (KAPA Biosystems), and sequenced on the Illumina NovaSeq platform with 50-nt paired-end reads ...
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bioRxiv - Cancer Biology 2021Quote: ... Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche). PCR amplification was performed as follows ...
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bioRxiv - Immunology 2020Quote: ... whose copy number was determined using the Cobas 6800 HIV-1 kit (Roche) was included in every RNA isolation/qPCR run ...
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bioRxiv - Genomics 2023Quote: ... The brain samples were homogenized in RIPA with 1% SDS and protease inhibitor cocktail (1 tablet per 10 ml, Roche complete, EDTA-free, Sigma-Aldrich) using a sonicator with a 10 s pulse at 40% amplitude.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were extracted by mixing 500 mg of ground tissue with 2.0 ml of extraction buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, cOmplete Mini EDTA-free Protease Inhibitor Cocktail [Roche, 1 tablet per 10 ml]), followed by brief sonication in an ultrasonic bath for 5 s ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Biochemistry 2019Quote: ... Fisher Bioreagents) (PBS-T) and re-suspended in 50 μl storage buffer (Blocking Reagent for ELISA, 11 112 589 001, Roche Diagnostics) supplemented with ProClin (4812-U ...
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bioRxiv - Microbiology 2020Quote: IL-6 release over time was studied in the Laboratoriumsmedizin of the Munich University using an ELISA (Roche, Elecsys IL-6).
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Cancer Biology 2021Quote: ... + 0,4 g/l of human albumin (Vialebex) with Liberase TL (Roche) 150 ug/ml and DNase 1 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
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bioRxiv - Genomics 2019Quote: ... using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture ...
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bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: Human genomic DNA of high molecular weight (Roche Diagnostics, Basel, Switzerland) were processed in a single replicate per DNA extraction protocol (Table 1) ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were pooled to 10 nM in EB buffer and the final concentration was determined using the Illumina Universal qPCR Amplification kit from Kapa Biosystems. All pooled samples were diluted to 4 nM ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng of cDNA were used for qPCR with the KAPA SYBR FAST qPCR Kit (KK4604, KAPA Biosystems, Wilmington, MA, USA) and the experiment run on the Biorad CFX96 system using the TqPCR protocol described in Zhang et al ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Genomics 2024Quote: ... We used 10 ng of DNA from two biological replicates to generate sequencing libraries using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10 µl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on an ABI 7500 fast machine operated with ABI 7500 software (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM MgCl2, 10 % v/v glycerol, 140 mM NaCl, 0.1 mM EDTA, 0.5 % Tween20, 1 mM DTT and Roche cOmplete-Ultra EDTA free protease inhibitor) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP40 and 10 mM Tris (pH 7.5)) supplemented with cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland). Samples were incubated on ice for 10 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... then immersed in 0.1 M maleate buffer (pH 7.5) for 10 minutes before blocking in 1% Blocking Reagent (Roche) for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed by the addition of 1000 μL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice to generate an average DNA fragment size of ∼250 bp ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM EDTA) supplemented with protease inhibitors (complete mini EDTA-free, Roche, 1 tablet / 50 mL lysis buffer). The lysed neurons were mixed with 4X Laemmli sample buffer and heated at 70°C for 10 minutes (stored at −20°C until use) ...