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Citations for Roche :
601 - 650 of 6590 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... plus 2% fetal calf serum (Dutscher)) supplemented with 50μg/mL DNAse and 10μg/mL collagenase D (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Total protein were extracted from cells and tissues using protein lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Protein concentrations were assayed by the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein extraction cells were resuspended in protein extraction buffer (1xPBS, 10% glycerol, 1mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail), and lysed in a bead beater ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extract was incubated overnight with anti-HA antibodies (Roche). After over-night incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were incubated with 30 µl Protein G-Agarose (Roche) per ml lysate for 1 hour at 4 °C and washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... followed by incubation with Protein G Agarose beads (Roche, 1124323301) (15μl ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl bed volume of Protein A Agarose (Roche 11134515001) was added the following day to each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitations were performed using Protein G Agarose beads (Roche, 1124323301) or anti-FLAG (M2 ...
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bioRxiv - Genetics 2022Quote: ... 40 μl of protein G agarose beads suspension (Roche 11243233001) (settled beads volume 20 μl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by Lumi-Light Plus detection reagent (Roche).
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bioRxiv - Microbiology 2021Quote: ... 100 µl of protein G-coupled agarose bead resin (Roche) were washed (2 × CL buffer 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 200 ng trypsin (Roche, Basel, Switzerland) shaking at 600 rpm at 37°C for 17 hours ...
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bioRxiv - Cell Biology 2020Quote: GFP-fusion proteins were detected using a monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted using RIPA buffer (Thermo) and proteinase (Roche) plus phosphatase (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Resuspended protein samples were digested with endoproteinases Lys-C (Roche) and trypsin (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and pre-cleared with protein A-agarose beads (Roche, Germany). Primary antibody incubations were carried out for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected with anti-HA antibody (Roche, Cat#11867423001).
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bioRxiv - Genomics 2019Quote: The gDNA libraries were prepared using a SeqCap EZ Human Exome Library v2.0 (Roche, Basel, Switzerland). Sequencing was performed with 100-bp paired-end reads by the HiSeq2000 sequencer (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790-4325), human PR rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.33 M D-sorbitol supplemented with one tablet (per 50 mL) of cOmplete protease inhibitor cocktail (Roche)] using a Waring blender ...
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested at 37°C with 630 µg/mL collagenase D (Roche) and 75 U/mL of DNase I (Sigma–Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by a 20-min incubation at 37°C in CSS containing 1.5 mg/ml Collagenase D (Roche), 0.6 mM EDTA ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions of the tumor were obtained after tumor digestion with 400U/mL of collagenase D (Roche) at 37C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... lung tissue was minced with a scalpel and digested enzymatically with 0.15 WU/mL of D-Liberase (Roche) and 800□U/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... Muscle was then digested in a 15 ml Falcon tube containing 2.4 U/ml Collagenase D (11088882001, Roche), 12 U/ml Dispase II (04942078001 ...
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bioRxiv - Genomics 2021Quote: ... The concentration of each 10x single cell and V(D)J library was determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the NovaSeq 6000 platform ...
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bioRxiv - Cell Biology 2022Quote: ... Inguinal stromal-vascular fractions (SVF) were isolated from 2-week-old C57BL/6J mice with collagenase D (Roche) digestion and differentiated as described previously34 ...
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bioRxiv - Immunology 2022Quote: ... they were digested for 30 minutes at 37°C in PBS 1X containing 1mg/mL Collagenase D (Roche), 100 U/mL DNAse I (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Lamina propria-resident immune cells from large intestine were isolated by digesting intestinal tissue with Collagenase D (Roche), Collagenase V (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... the lung was perfused with PBS and digested for 45 minutes with 625μg/mL collagenase D (Roche, 11088875103) and 75U/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Histological staining included terminal transferase mediated d-UTP nick end labelling (TUNEL) with Co/Ni enhancement (Roche, UK) performed following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cells were isolated from the brain by mechanical disruption followed by collagenase D digestion (Roche Pharmaceuticals, Mannheim, Germany). The resulting cell suspension was resuspended in 40% Percoll (GE Healthcare Biosciences ...
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and digested for 45 minutes with 625μg/mL collagenase D (Roche 11088875103) and 75U/mL DNase I (Sigma D4527) ...
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bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
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bioRxiv - Bioengineering 2024Quote: ... the infected lungs were transferred to gentleMACSTM C tubes and homogenized on a gentleMACSTM tissue dissociator (“m_lung_01” program) using HEPES buffer (2 mg/ml collagenase D (Roche) and 80 U/mL DNAse I (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... and pancreatic tumor bearing mice by mechanical separation of the tumor followed by digestion with an enzymatic cocktail consisting of 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I (all from Roche) in PBS at 37°C for 2-3h with rotation at 600rpm ...
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bioRxiv - Immunology 2019Quote: ... The chopped tissue was then put in a mixture of 1mg/ml collagenase D and 0.1mg/ml DNase I (Roche) in HBSS (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...