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Citations for Roche :
451 - 500 of 5398 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... RNA mRNA libraries of template molecules suitable for high throughput DNA sequencing were created using the KAPA mRNA HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were hybridized with the MVC VP2 genome probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA level was detected using the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Cat. 11585614910).
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Molecular Biology 2022Quote: ... The clones were washed off the plates and the total plasmid was extracted using High-pure plasmid isolation kit (Roche).
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bioRxiv - Microbiology 2023Quote: ... and the membrane was treated with the DIG-High Prime DNA Labeling and Detection Starter kit II (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from sigmoid colon tissue using a High Pure RNA Isolation kit (Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted at 120 h or 144 h post aggregation using the High Pure RNA Isolation Kit (Roche 11828665001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNAs were obtained by reverse transcription of 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from 200 µL of indicated cell supernatants using a High Pure Viral RNA Kit (Roche, Germany). The extracted RNA was eluted in 50 µL of elution buffer ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Neuroscience 2019Quote: ... barcoded and enriched using the NimbleGen SeqCap EZ Human Exome Library v2.0 enrichment kit (Roche NimbleGen, Madison, WI, USA). Purified and quantified library pool was subsequently sequenced on an Illumina HiSeq 2000 sequencing instrument (Illumina ...
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bioRxiv - Plant Biology 2019Quote: ... The fine powder was resuspended with the extraction buffer (50 mm Tris, pH 8, 8 M urea, and 0.5% SDS) supplemented with protease inhibitor (Roche Diagnostics GmbH, Mannheim, Germany), and homogenized with a Dounce homogenizer ...
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The library size distribution was checked on an Agilent high-sensitivity DNA chip and the library quantified using the KAPA library quantification kit (KAPA Biosystems). For each reaction ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.05% (v/v) Tween-20) and processed through the spin columns from High Pure Viral Nucleic Acid Large Volume kits (Roche, Switzerland). Purified DNA was eluted in either 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Total RNA was isolated at day 7 or 14 of MSCs differentiation using the High Pure RNA Isolation kit (Roche Diagnostics). For tissues ...
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bioRxiv - Plant Biology 2019Quote: ... An Illumina DNAseq library was constructed for polishing the PacBio based assembly using 1ug of DNA the same high molecular weight DNA prep with the KAPA HyperPrep Kit (Kapa Biosystems). The Illumina DNAseq library was sequenced on an Illumina HiSeq4000 under paired end mode (150 bp ...
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bioRxiv - Genomics 2021Quote: Genomic DNA libraries were constructed for high throughput sequencing using the KAPA hyper Prep Library Preparation Kit (Kapa Biosystems, Woburn, MA). Mechanical DNA shearing was performed with the Covaris S220 through microTube-50 AFA Fiber Screw-Cap (Covaris® ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were washed twice in cold PBS and total RNA was extracted using a High Pure RNA isolation kit (Roche 11828665001). Reverse transcribed RNA was used for sequencing by the University of Lausanne ...
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bioRxiv - Plant Biology 2021Quote: ... Hybridization was conducted according to the procedures described in the DIG High Prime DNA Labeling and Detection Starter kit II protocol (Roche®), using a 725-bp C-terminal TalCMAI1 amplicon as probe (Yu et al ...
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bioRxiv - Microbiology 2020Quote: ... DNA extractions performed at the MPI-SHH substituted the column apparatus from the High Pure Viral Nucleic Acid Large Volume Kit (Roche, Switzerland) in place of the custom assembled Zymo-reservoirs coupled to MinElute (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... as per the manufacturers protocol. Libraries were quantified using Qubit (Life Tech Qubit high sensitivity DNA kit Cat. #Q32854) and Kapa (Kapa Biosystems, KAPA Library Quantification Kit ...
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bioRxiv - Cell Biology 2021Quote: ... the small fragments from the cDNA amplification are purified and the library is barcoded and amplified using PCR with the KAPA high fidelity PCR kit(KAPA Biosystems). After all libraries are ready their quality and size are measured using the bioanalyzer before being sequenced on a NEXTseq (Illumina).
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bioRxiv - Immunology 2021Quote: ... Quality of libraries was determined via Agilent 4200 Tapestation using a High Sensitivity D1000 ScreenTape Assay kit and quantified by KAPA qPCR (KAPA BioSystems). Approximately 60–80 million paired-end 150 bp reads were generated per sample using Illumina HiSeq 4000 platform ...
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bioRxiv - Genetics 2022Quote: ... Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics). Washing conditions was performed according to Huang et al (Huang et al ...
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bioRxiv - Microbiology 2019Quote: ... The probe was amplified by PCR with digoxigenin-dUTP from the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics), using the glms sequence as a template ...
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bioRxiv - Microbiology 2019Quote: Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The probes were labeled with digoxigenin (DIG) according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). Northern blot procedures were performed as previously described (Jiang et al. ...
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bioRxiv - Plant Biology 2019Quote: ... were used as templates to synthesis DIG-labeled probes according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). ULTRAhyb®-Oligo Hybridization Buffer (Ambion ...
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bioRxiv - Genomics 2020Quote: ... Libraries were assessed using Bioanalyzer DNA High Sensitivity chips and quantified by quantitative PCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were pooled in equimolar quantities and paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150 bases in length.
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bioRxiv - Cell Biology 2020Quote: ... Library size distribution was checked on an Agilent high-sensitivity DNA chip and the concentration of the indexed library was determined using the KAPA library quantification kit (KAPA Biosystems) according to the manufacturer’s instructions on the StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA from twenty-four females from each of the four groups was extracted using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labeling and detection starter Kit I (11745832910 Roche Germany). The Myb gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labelling and detection starter Kit I (11745832910 Roche Germany). The MYB gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Biochemistry 2019Quote: ... and S278E amino acid substitutions were introduced into human RACK1 using the QuickChange II XL site-directed mutagenesis kit (Roche). All plasmids are available upon request.
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...