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Citations for Roche :
251 - 300 of 956 citations for Human Carboxypeptidase M CPM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The cell pellets were mixed with 10-fold volume of sucrose buffer (0.25 M sucrose; protease and phosphatase inhibitor cocktails (Roche)) and homogenized until a completely homogenous solution was obtained ...
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bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
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bioRxiv - Immunology 2022Quote: ... were lysed in 400 μl lysis buffer in urea buffer (8 M urea, 50 mM Tris pH 8.0, 1 mM TCEP) with protease (cOmplete mini EDTA free, Roche) and with phosphatase inhibitors (PhosStop ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old tmk1-1 roots expressing TMK1::TMK1-FLAG were grounded to powder in liquid nitrogen and homogenized in ice-cold sucrose buffer (20 mM Tris–HCl pH 8, 0.33 M sucrose, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... the water was aspirated and Triton X-100 buffer (Triton X-100, 0.5 M EDTA, PBS, ddH2O) with phosphatase (Roche) and protease (Roche ...
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bioRxiv - Immunology 2023Quote: ... B-R18 labelled A1 EVs were resuspended in PBS buffer (0.2 M NaCl) containing a protease inhibitory cocktail (Complete Protease Inhibitory Tablets, Roche) and were stored at −20°C.
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were resuspended in microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche)) and repelleted by centrifugation at 100□000 g for 30 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 1 ml SEH buffer (20 mM HEPES pH 7.4, 1 mM EDTA, 0.6 M Sorbitol, 1x cOmplete™ Tablets mini EDTA-free protease inhibitor [Roche]). Resuspended yeast cells were transferred into screw cap tubes containing 500 µl glass beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2024Quote: ... Cell pellets were resuspended in lysis buffer (0.5 M NaCl, 20 mM Na3PO4 pH 7.4 and cOmplete™ Protease Inhibitor Cocktail (Roche) and then lysed using sonication ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human samples using TriPure Isolation Reagent (Roche). RNA integrity (RIN ...
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bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... eosin and antibodies for human keratins AE1/AE3 (760-2135; Roche diagnostics). Metastasis quantification was reached through a review of 17 digitalized slides ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A Agarose (Roche) were washed and equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membranes (Roche) in tris-glycine buffer using semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A beads (Roche) was added to lysates and incubated with rotation for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were prepared by mechanical lysis using glass beads in presence of protein inhibitors (Complete EDTA-free, Roche) and 2x phosphatase inhibitors PhosStop (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Cancer Biology 2021Quote: ... pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 1× Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Molecular Biology 2020Quote: ... 100,000 cells were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to activated ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... and then resuspended in 200 μL of SCE buffer (1 M sorbitol, 10 mM EDTA, 10 mM DTT, 100mM sodium citrate, 1 Roche mini-EDTA-free protease inhibitor tablet per 50 mL ...
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bioRxiv - Molecular Biology 2019Quote: ... Solidified plugs were transferred in 14 ml tubes with round bottom and incubate with 400 µl/plug of PK buffer (EDTA 0.125 M [pH 9.5], Sarkosyl 1%, PK 1 mg/ml (Roche, ref# 03115801001) and incubated 1-2 hours at 37°C with gentle agitation ...
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bioRxiv - Genomics 2019Quote: ... The supernatants from the digestion and initial decalcification step were purified using a 5 M guanidine-hydrochloride binding buffer with a High Pure Viral Nucleic Acid Large Volume kit (Roche). The extract was eluted in 100 μl of a 10mM tris-hydrochloride ...
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bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
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bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
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bioRxiv - Neuroscience 2021Quote: ... were dissociated onto poly-d-lysine (50 ug/mL diluted in 0.1 M Borate Buffer, pH 8.6) and laminin (10 ug/mL; Roche, Basel, Switzerland) coated 6 well plastic plate ...
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bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...