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Citations for Roche :
1 - 50 of 431 citations for Human C17orf112 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with Lpd-specific or scramble shRNA as control using Fugene HD (Roche) transfection reagent ...
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bioRxiv - Bioengineering 2019Quote: ... Human Fibronectin (Roche). Agar low melting point ...
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bioRxiv - Cell Biology 2021Quote: ... human plasma fibronectin (Roche) was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF-7 shRNA and MCF-7 shFTH1 as well as H460 shRNA and H460 shFTH1 using the High Pure RNA isolation kit (Roche) according to the manufacturer’ ss instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with SP-RFP and either shRNA SP-GFP or empty vector (pCDNA3) using a lipofection method (X-tremeGENETM HP DNA Transfection Reagent, Roche) at a ratio 1:3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Cell growth of shRNA-treated cell lines was assayed either through crystal violet staining or using the Promega CellTiter-Glo proliferation kit (Roche) or alternatively by live-cell confluence measurements with the IncuCyte (Essen BioScience) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was isolated using the High Pure Plasmid Isolation kit (Roche). Sequences were confirmed by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... and total plasmid was isolated using High-pure plasmid isolation kit (Roche). The dro genes were minimally PCR amplified (10 cycles ...
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bioRxiv - Cancer Biology 2022Quote: Gene expression knockdown in response to shRNA expression was verified by RT-QPCR as previously described (Saurty-Seerunghen et al 2019) using the LightCycler480 (Roche, France) and the SYBR Green PCR Core Reagents kit (Bimake.com) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Microbiology 2023Quote: ... and plasmid DNA was isolated using a High Pure Plasmid Isolation Kit (Roche). For amplification of genetic elements ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid DNA was extracted using the High Pure Plasmid Isolation Kit (Roche, Basel, Switzerland). Fungal DNA was extracted as previously described (Byrd et al. ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
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bioRxiv - Molecular Biology 2021Quote: ... and human genomic DNA (Roche Diagnostics, Germany) were used as templates for the experiments in Figure 2 and S1a ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD20 human Ab (obinutuzumab, Roche). Relevant negative controls were performed using isotype antibodies and cells incubated with anti-CD20 human and anti-CD20 mouse antibody served as a positive control ...
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bioRxiv - Immunology 2023Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmid DNA was extracted from wild-type strains using Genopure Plasmid Midi Kit (Roche Diagnostics GmbH, Mannheim, Germany). Plasmids were reintroduced by electroporation and their presence was confirmed by PCR assays for gene blaCTX-M and for FII and multiplex (FIA ...
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bioRxiv - Cell Biology 2022Quote: ... a plasmid encoding the Flp recombinase (pOG44) was co-transfected with the FRT-based plasmids using XtremeGene9 (Roche) via reverse transfection method into IMCD3 Flp-In cells as described (Liew et al. ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Microbiology 2023Quote: ... the High Pure Plasmid Isolation kit (Roche), and the GeneJET PCR purification kit (Thermo Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... 10 ng/ml recombinant human EGF (Roche, Ireland), 1 μg/ml hydrocortisone (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated by co-transfecting HEK293T cells with the lentiviral construct pCMV-dR8.91 (Δ8.9) plasmid and the pMDG envelope-expressing plasmid using X-tremeGENE 9 DNA Transfection Reagent (Roche, 6365779001). Viral supernatant was collected within 24-48 h post-transfection for subsequent infection of the target cells.
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bioRxiv - Systems Biology 2019Quote: ... along with the packing plasmid psPAX2 (16.2 μg) and envelope plasmid pMD2.G (1.8 μg) were transfected using Fugene 6 (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Large scale preps of bacteria cultures containing the sgRNA plasmid library were harvested using the Genopure plasmid maxi kit (Roche). SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A ...
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bioRxiv - Cancer Biology 2022Quote: ... For the infection the virus was generated in 293FT cells transfected with PAX2 and pMD2.G plasmids together with the pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W plasmid using the X-tremeGENE reagent (Roche). MCF7 cells (without expression of dCCAS9-KRAB or the pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W plasmid) ...
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bioRxiv - Cancer Biology 2022Quote: A vector control plasmid and WT and mutant Plk3 expression plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Roche) as described previously (Li et al. ...