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Citations for Roche :
551 - 600 of 1418 citations for High mobility group protein B1 HMGB1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 5’UTRs and 3’UTRs amplifications were performed using either Expand High Fidelity PCR System (Roche) or KAPA HiFi PCR system (KAPA Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: For making all constructs DNA was amplified from the template using Expand High-Fidelity Polymerase (Roche) unless stated otherwise and all constructs were verified by sequencing ...
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bioRxiv - Biochemistry 2022Quote: DNA from liver and cell pellets was isolated using High Pure PCR Template Preparation Kit (Roche). DNA concentrations were measured by Nanodrop 2000c Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana using the primers as indicated in Supplemental Table S9 with high-fidelity Taq enzyme (Roche) and cloned into pGGA000 or pGGC000 GreenGate entry vector (Lampropoulos et al. ...
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bioRxiv - Microbiology 2022Quote: ... Viral DNA was then extracted using the High Pure Viral Nucleic Acid Kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA synthesis was performed by reverse transcription using the Transcriptor High Fidelity cDNA-Synthesis Kit (Roche) according to the manufacture’s protocol ...
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bioRxiv - Pathology 2023Quote: ... 2 canine gastrointestinal biopsies and 5 canine normal tissues (High Pure PCR Template Preparation Kit, Roche Applied Science ...
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bioRxiv - Biochemistry 2023Quote: ... All PCRs were performed using the Expand High Fidelity PCR System (Roche Diagnostics GmbH, Mannheim, Germany) and primers were designed using the online tool at www.leishgedit.net ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from ES cells using the High Pure RNA Isolation Kit (Roche Diagnostics) and RTPCR performed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... agarose or Matrigel 144 hrs post aggregation using the High Pure RNA Isolation Kit (Roche 11828665001) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the membranes were incubated with anti-HA-peroxidase high-affinity monoclonal rat antibody (clone 3F10; Roche) 1:1000 diluted in 2.5 % (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNA was purified using the High Pure PCR Product Purification Kit (Roche Applied Science) [44].
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bioRxiv - Microbiology 2023Quote: ... Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche, Branford, CT, USA) with PCR amplification conditions optimised for each plant tissue (Table S2) ...
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bioRxiv - Microbiology 2024Quote: RNAs were extracted 48 hours after RNP transfection using the High Pure RNA Isolation Kit (Roche) according to instructions of the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: RNA extraction from cultured cells has been performed using the High Pure RNA Isolation Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... were stained with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... with primer set #3 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BspEI and KpnII sites respectively ...
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bioRxiv - Pathology 2024Quote: ... and detection were performed using DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the instruction manual.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies dilutions were as follow: high affinity rat anti-HA (clone 3F10, Roche; 1:1,000), mouse anti-FIKK4.2 (1:1,000 ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μl of slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μL of a slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were detected using ECL lumi-light plus (Roche) and images acquired using camera system (Chemitouch Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Roche) in tris-glycine buffer at 110 V for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Cancer Biology 2019Quote: BSA Protein Assay Kit (A8020-5, Roche, Basel, Switzerland) was applied to measure the protein concentration after lysing BC cells with RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 1/10 volume of Protein-G agarose beads (Roche) was then added and the sample rotated for 30 min at RT °C ...
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bioRxiv - Immunology 2019Quote: Proteins were digested on beads using trypsin (Roche, Germany) in 6 M Urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 80V for 1.5 hours ...
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bioRxiv - Biochemistry 2019Quote: ... and then incubated with protein A–agarose beads (Roche) for 4 h ...
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bioRxiv - Genomics 2019Quote: ... protease inhibitors) and precleared with protein A beads (Roche) at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using Protein A agarose beads (Roche). DivJ was immunoprecipitated using an anti-DivJ antiserum (15) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were semi-dry blotted onto PVDF membranes (Roche), which were subsequently blocked in 5 % milk in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with Protein G beads (Roche, 1124323301) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 30V for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Neuroscience 2021Quote: ... Then homogeneous protein G-agarose suspension was added (Roche) to the suspension ...
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bioRxiv - Pathology 2019Quote: ... Proteins were detected using polyclonal antibodies against GFP (Roche), Histone H3 (GeneTex ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to 50µl bed volume Protein A agarose (Roche) per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 1x protein inhibitor cocktail (Roche), and incubated at 25ºC for 1 h on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with protein G-agarose beads (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred onto PVDF membranes (Roche, Basel, Switzerland), and Western blot detection was carried out as previously described [32,33] ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2023Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Roche), nonspecific binding was blocked by incubation with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 400 µg of protein were reduced using DTT (Roche, Cat#10708984001 ...