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Citations for Roche :
251 - 300 of 815 citations for Hepatitis E Virus Capsid Protein ORF2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Biochemistry 2020Quote: ... and the presence of enzymes were confirmed by immunoblot using the anti-His6 antibody conjugated to peroxidase (BMG–His-1 monoclonal antibody) (Roche, Mannheim, Germany).
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bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Thee injections of either an anti-GluA2 antibody (clone 15F1, gift from E. Gouaux) or a monoclonal anti-GFP IgG1-K (Roche, 11814460001) were targeted to the L2/3 of S1 (−0.1 to 0.3 mm dorsoventral) ...
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bioRxiv - Microbiology 2022Quote: ... with the IDT E Assay First Line Screening primers and probe (Cat # 10006804) on a LightCycler 96 System (Roche, Cat# 05815916001).
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bioRxiv - Plant Biology 2020Quote: ... Protein was equally loaded on SDS-PAGE for immunoblotting and the HA tag was detected using an anti-HA antibody (Roche, #11867423001 www.sigmaaldrich.com) and Femto chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed on white 384-well plates (E&K Scientific, Santa Clara, CA) using Roche Lightcycler 480 (Roche Molecular Systems, Inc.). Each reaction contained 1 µL of cDNA from the RT reaction ...
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bioRxiv - Immunology 2023Quote: The retroviral packaging cell line Platinum-E was co-transfected with pMXs-SOX2-IP or pMXs-IP using the DNA Transfection Reagent X-TremeGene 9 (ROCHE, Cat# 06365779001) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G agarose beads (Roche #11719416001) pre-blocked with salmon sperm (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... together with protein A agarose (Roche) overnight at 4 ℃ ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein G agarose beads (11243233001, Roche) were added to the lysates and incubated for additional 4 hours at 4°C with rotary agitation ...
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bioRxiv - Neuroscience 2021Quote: ... homogeneous protein G-agarose suspension (Roche) was added to the suspension ...
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bioRxiv - Microbiology 2019Quote: ... Protein G-conjugated agarose beads (Roche) or PrecipHen for chicken antibodies (Aves Labs ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor cocktail (Roche, Mannheim, Germany) and then sonicated ...
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bioRxiv - Genetics 2021Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... and Protein G-Agarose (Roche, 11243233001) were added to each sample and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Neuroscience 2020Quote: DNA from the patient and his parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
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bioRxiv - Molecular Biology 2022Quote: ... the first of which assessed aerosol formation when handling potentially infectious samples using a panel of cobas e analyzers (conducted at Roche Diagnostics, Penzberg, Germany). The second sub-study assessed aerosol formation when handling potentially infectious samples using cobas e analyzers in an end-to-end laboratory workflow (conducted at Roche Diagnostics International ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Genomics 2020Quote: ... and high-density lipoprotein cholesterol [HDLC]) were determined enzymatically either with the COBAS Integra 400 Plus or Modular-E analyzers (Roche Diagnostics, Indianapolis, IN). Methods were standardized to in-house and other appropriate reference methods (e.g. ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801; HA: Roche #11867423001; VSVG: Sigma #SAB4200695) in a total volume of 500 μL 50 mM MES (pH 5.0) ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A Agarose (Roche) were washed and equilibrated in lysis buffer ...