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Citations for Roche :
151 - 200 of 270 citations for Hepatitis B Virus E Antigen HBeAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: One-step simplex real-time quantitative RT-PCR amplifications were performed using Multiplex RNA Virus Master Kit (Roche Diagnostics, France) for influenza A ...
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bioRxiv - Immunology 2020Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Microbiology 2022Quote: A labeled DNA probe for the new virus derived from Mr was prepared using a commercial PCR DIG-labeling mix (Roche)according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The level of secreted prostate specific antigen (PSA) and T3 in the growth medium was determined using a validated Elecsys electrochemiluminescence immune assay (Roche, Rotkreuz, Switzerland) on a Cobas e601system (Roche ...
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bioRxiv - Immunology 2023Quote: ... The formalin-fixed paraffin- embedded (FFPE) tissue sections underwent deparaffinization and heat-mediated antigen retrieval on the Ventana Discovery Ultra auto-stainer platform (Roche Diagnostics, Canada), following the below instructions ...
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bioRxiv - Neuroscience 2023Quote: ... An electrochemiluminescence immunoassay was used to measure salivary cortisol concentrations (Cobas e 601, Roche Diagnostics, Numbrecht, Germany). The intra- and interassay variations for cortisol were below 10%.
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bioRxiv - Plant Biology 2019Quote: ... Seeds were selected on germination medium containing 15 µg ml-1 Hygromycin B (Roche) or 50 µg ml-1 Kanamycin (ProGPT1-GPT1_N-long mat ...
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bioRxiv - Developmental Biology 2020Quote: Mesoderm/Cardiac: Embryoid bodies (EBs) were generated from ESC lines using collagenase B (Roche) and cultured in RPMI 1640 base media (ThermoFisher ...
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Interdependent Iron and Phosphorus Availability Controls Photosynthesis Through Retrograde SignalingbioRxiv - Plant Biology 2021Quote: ... we used adapters from the KAPA Biosystems Single-Indexed Adapter Set A+B (Roche). Before pooling the libraries ...
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bioRxiv - Cell Biology 2020Quote: ... iBAT digestion buffer was prepared as follows: 15 mg collagenase B (Roche, Cat#11088831001) was dissolved in 5 mL of 2X master mix consisting of 125 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... After physical disaggregation and digestion with 2 mg/ml collagenase B (Roche, CA, USA), the enzymatic activity was stopped by diluting with PBS 1X ...
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bioRxiv - Cell Biology 2023Quote: ... productively edited cells were selected with 0.3 mg mL-1 hygromycin B (Roche, 10843555001). Homozygously edited clones were identified using the same genotyping strategy as described above and cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... All reverse transcriptase qPCR assays for a given target were performed simultaneously for all samples using the LightCycler Multiplex RNA Virus Master Kit (Roche diagnostics). Each sample was normalized with the geometric mean of the quantification cycle (Cq ...
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bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Genomics 2020Quote: ... targeting the nCoV2 specific ORF1ab (RdRp) and pan-sarbeco specific E genes on LightCycler® 480 System (Roche) and ABI 7500 Fast DX (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: Troponin T plasma levels were quantified using an electrochemiluminescence immunoassay analyzer “ECLIA” (cobas e 801 immunoanalysis system, Roche) in the hematology laboratory of the Centre Hospitalier Universitaire Vaudois (CHUV) ...
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bioRxiv - Microbiology 2021Quote: We then targeted the N-(nucleoprotein) gene of SARS-CoV-2 virus with Roche Lightcycler 480 RNA Master Hydrolysis Probes (Roche Basel, Switzerland) with primers developed in house ...
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bioRxiv - Microbiology 2020Quote: We obtained OP and NP samples from one hospitalised COVID-19 patient who had tested virus-positive three weeks earlier (cobas® SARS-CoV-2 test, Roche diagnostics), from three individuals who had previously tested SARS-CoV-2 positive ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Neuroscience 2019Quote: ... Tongues were removed and an enzyme solution containing 0.7mg/mL Collagenase B (Roche, Basel, Switzerland), 3mg/mL Dispase II (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM MgCl2 (buffer B) containing 2 mM PMSF and Complete protease inhibitor cocktail (Roche). After centrifugation ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 1.5mL per 0.5g tissue (4mg/mL dispase II [Sigma D4693], 10mg/mL collagenase B [Roche 11088831001] in Hams-F10 [Multicell ...
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bioRxiv - Molecular Biology 2022Quote: ... Muscle tissues were minced and digested in type II collagenase and dispase B mixture (Roche). Digestion was neutralized by adding growth media containing F-10 Ham’s medium (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 10 mg/ml of collagenase B and 2.4 U/ml Dispase II (Roche Diagnostics) for 30 min at 37°C under agitation and passed through a 30 μm cell strainer (Myltenyi Biotec) ...
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bioRxiv - Cancer Biology 2019Quote: Real-time cell proliferation was measured using an E-plate 16 and a RTCA DP Analyser (xCelligence System, Roche), which uses gold microelectrodes fused to the bottom surface of a microtiter plate well ...
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bioRxiv - Cancer Biology 2023Quote: ... in a total volume of 10 μl buffer E (25mM Tris, pH 8.0, 300mM NaCl) for 10 min at room temperature in 384-well opaque (Roche) PCR plates ...
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... the number of cellular genomes was determined using the b-globin genekit (Roche Diagnostics, Manheim, Germany). Patient sample was independently analyzed in duplicate ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells and B cells were activated for 48h with 10U/ml IL-2 (Roche) and 2 μg/ml phytohemagglutinin (PHA ...
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bioRxiv - Systems Biology 2021Quote: ... for 2 hours followed by overnight digestion with 1.5 mg/ml collagenase B (Roche Diagnostics, Switzerland) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... On Day 0 (start of differentiation) hPSCs were treated with 1 mg/ml Collagenase B (Roche) for one hour ...
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bioRxiv - Pathology 2021Quote: ... a portion of each tissue was fixed separately for histologic processing and collagenase B (Roche, Switzerland) digestion for flow cytometry analysis ...
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bioRxiv - Cell Biology 2022Quote: ... muscles were mechanically disaggregated and dissociated in DMEM 1% P/S media containing collagenase B (Roche) 0.2% and Calcium dichloride (CaCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PlesSat01-48 and PlesSat02-200-B were labelled with digoxigenin-11-dUTP (both Roche, Mannheim, Germany).
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bioRxiv - Pathology 2024Quote: ... containing 10 mg/ml of collagenase B and 2.4 U/mL Dispase II (Roche Diagnostics GmBH) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The region around the E-Box was amplified with primers outside of the homology regions using Kappa HiFi HotStart ReadyMix (Roche) and cloned into pCR-Blunt II-TOPO (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in PBS-E LB (1x PBS, 1% TX100, 1 mM EDTA, 1 mM DTT, Protease Inhibitor cocktail (Roche) and Phosphatase Inhibitor solution (Sigma)) ...
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bioRxiv - Neuroscience 2020Quote: ... Real time quantitative PCR was performed on the E gene as described recently (Corman et al., 2020) on a Lightcycler LC480 (Roche).
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bioRxiv - Microbiology 2020Quote: ... previously published assay (“one-step real-time RT-PCR E assay”) using the One-Step RT-qPCR kit (Roche Diagnostics). For each run ...
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Molecular Biology 2022Quote: ... The second sub-study assessed aerosol formation when handling potentially infectious samples using cobas e analyzers in an end-to-end laboratory workflow (conducted at Roche Diagnostics International ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μm sections were stained with H&E and Masson trichrome (Trichrome II Blue staining kit at Nexus special stainer; Roche). Immunohistochemical analysis was performed on cryosections and paraffin sections using a Bench Mark XT automatic staining platform (Ventana ...
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Muscles were dissociated and digested in RPMI medium containing 0,2% of collagenase B (Roche Diagnostics GmbH 11088815001) at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... pH: 7.25) and finally homogenized in Buffer B with protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN) using a Polytron ...
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bioRxiv - Microbiology 2019Quote: ... were transfected with 20 ng pNF-□B-Luc and 3 ng pRL-TK using FuGENE 6 (Roche). Transfected cells were incubated for 24 h (37°C ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B-0.3 (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 0,3%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).