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Citations for Roche :
251 - 300 of 4123 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... and incubated for 12 h with addition of alkaline phosphatase coupled with anti-digoxigenin antibody for amha and anti-Fluorescein antibody for amhby (1:2000, Roche Diagnostics Corp, Indianapolis, IN). Samples were then washed with PBS solution ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Tween 20) for 20 min followed by 1 h incubation with alkaline phosphatase-anti-digoxigenin antibody (1:500, Roche, 1093274). Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: The following conjugated antibodies were used: sheep polyclonal anti-digoxigenin alkaline phosphatase (1:2000 - 1:5000; Roche Life Science, Cat#11093274910), mouse monoclonal anti-βACTIN (clone AC-15 ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mg/ml bovine serum albumin dissolved in TBST) for 1 h and subsequently incubated with anti-DIG-Fab antibody coupled to alkaline phosphatase (Roche: 11093274910) in blocking solution (1 in 5000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were equilibrated in alkaline phosphatase buffer (NTMT) and developed with NBT/BCIP solution (Roche). After the color reaction ...
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bioRxiv - Neuroscience 2020Quote: ... Alkaline phosphatase detection was done using BCIP/NBT colorimetric revelation (Roche Life Science, Cat#11681451001).
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bioRxiv - Neuroscience 2020Quote: ... each slide was incubated with 100 µL antidioxigenin alkaline phosphatase-conjugated antibody (Roche, Mannheim, Germany) diluted 1:500 in 1% blocking reagent in TBS plus 0.03% Triton-X100 at 37 °C for 1 h ...
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bioRxiv - Genomics 2024Quote: ... pH 9.5) and alkaline phosphatase activity was visualized by incubating in BM purple reagent (Roche) in the dark with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed in PBT and alkaline phosphatase buffer and stained with BM Purple (Roche) staining solution ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Microbiology 2020Quote: ... The alkaline phosphatase conjugated anti-mouse secondary antibodies were used for hybridization and for detecting signals BCIP/NBT substrates (Roche Biochemical, Mannheim) was used.
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight with 1:1,000 diluted alkaline phosphatase-conjugated anti-DIG sheep antibody (11-093-274-910; Roche Diagnostics, Basel, Switzerland) and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP ...
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bioRxiv - Genetics 2021Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... alkaline phosphatase activity was detected by incubation with 375 mg/mL nitroblue tetrazolium chloride (Roche Diagnostics) and 188 mg/mL 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2022Quote: ... rinsed 2 times for 5 minutes with alkaline phosphatase buffer and stained with NBT/BCIP (Roche) as described previously (Kraus et al. ...
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bioRxiv - Genetics 2019Quote: ... Detection of DNA fragments was carried out using CDP-Star Chemiluminescent Substrate for Alkaline Phosphatase (Roche).
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bioRxiv - Developmental Biology 2019Quote: ... the sections were incubated overnight at 4°C with alkaline phosphatase–conjugated antibodies to digoxigenin (Roche) at a dilution of 1:2000 in the same solution ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-digoxigenin alkaline phosphate linked fab fragments (Roche) were used to detect probes by incubating the samples with a 1:2000 solution in MAB-T + 0.1 % bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... anti-proteases/phosphatases (Roche), RNase (200 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and the residual Placental alkaline phosphatase activity was visualized by incubating the embryos with NBT/BCIP (Roche) in the same buffer at 4°C for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... hybridized with sense or antisense probes overnight at 65 °C and incubated with the alkaline phosphatase-coupled sheep anti-digoxigenin antibody (1:2000, Roche Applied Science, Manheim, Germany) overnight at 4 °C ...
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bioRxiv - Zoology 2020Quote: ... Next the 1% BSA in PBST is replaced with the same solution to which both sheep anti-digoxygenin Fab-fragments conjugated to alkaline phosphatase (Roche Diagnostics GmBH, Mannheim, Germany) in a 1:1,000 dilution and SIFamide antiserum in a 1:2,000 dilution have been added ...
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bioRxiv - Bioengineering 2021Quote: ... After the addition of the chemiluminescent substrate for alkaline phosphatase CDP star® ready to use reagent (Roche) at a 1:4 ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche; 1:100 dilution). Samples were mounted using SlowFade Diamond Antifade with DAPI mounting media (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Developmental Biology 2022Quote: ... The alkaline phosphatase activity was visualized by incubating the specimens in NTMT containing 0.07 mg/ml nitroblue-tetrazolium chloride (Roche), and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
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bioRxiv - Neuroscience 2019Quote: ... the samples were immersed into the goat anti-rabbit IgG (1:10000, Roche Group, USA) to incubate at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Labelling efficiencies were tested by dot blotting with anti-digoxigenin alkaline phosphatase conjugate and CSPD chemiluminescence substrate for alkaline phosphatase from DIG High Prime DNA Labelling and Detection Starter Kit II (Roche) according to the manufacturer`s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... SOS1•RAS complex was formed by incubating SOS1 and RAS at a stochiometric ratio of 1:3 overnight at 4° with 20 mM EDTA and alkaline phosphatase (Roche). The complex was then purified by gel filtration ...
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bioRxiv - Pathology 2023Quote: ... Bound alkaline phosphatase was visualized with nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP; 11681451001, Roche). The reaction was stopped by incubation in buffer 4 (10 mM Tris and 1 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... or Ultra View Universal Alkaline Phosphatase Red Detection Kit (yielding pink signals) using standard machine settings (all solutions were from Ventana, Roche). Counterstaining (light blue background ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
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bioRxiv - Cell Biology 2020Quote: ... and anti-phosphatases (PhosphoSTOP, Roche®). After incubation for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... at 4°C with alkaline phosphate anti-DIG (1:2000, Roche). For chromogenic revelation ...
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bioRxiv - Developmental Biology 2023Quote: ... visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche) and subsequent reaction with 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium before examination using Nomarski optics on a Zeiss Axiophot.
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bioRxiv - Cell Biology 2020Quote: ... 10% Glycerol containing anti proteases and anti phosphatases (Roche) and sent for analysis at the Reverse Phase Protein Array Core Facility at MD Anderson ...
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bioRxiv - Immunology 2021Quote: ... supplemented with anti-protease and anti-phosphatase tablet (Roche). 20µg of each sample was loaded into a 4-12% Bis-Tris Gel (Invitrogen ...