Labshake search
Citations for Roche :
151 - 200 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed with 1 μg of purified RNA using Transcriptor First Strand cDNA synthesis KIT (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared from 1 µg of total RNA using a Kapa mRNA HyperPrep kit (Roche, KK8580) per the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in 384-well LightCycler 480 plates (Roche, Basel, Switzerland) in a LightCycler 480 thermocycler (Roche ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reactions were run in a LightCycler® 480 Multiwell Plate 384 machine (Roche). Relative fold changes were calculated using ddCT calculations and by calculating standard deviations ...
-
Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue controlbioRxiv - Microbiology 2019Quote: Reactions were run as 384-well plates in a LightCycler 480 II (Roche). qPCR-HRM was performed in 10 µL reactions containing 2 µL of DNA ...
-
Distinct Cellular Strategies Determine Sensitivity to Mild Drought of Arabidopsis Natural AccessionsbioRxiv - Plant Biology 2020Quote: ... on 384-well plates with a LightCycler 480 SYBR Green I Master (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... All reactions were performed in 384-well plates on the LightCycler480 instrument (Roche) in a 5 µL reaction volume using 250 nM primer concentrations ...
-
bioRxiv - Immunology 2022Quote: ... Tumors were transferred into 6-well plates containing 0.25mg/mL LiberaseTL (Roche, 5401020001) and 0.33mg/mL DNase (Sigma-Aldrich DN25-10MG ...
-
bioRxiv - Cell Biology 2023Quote: ... All qPCR reactions were performed on a LightCycler 480 Multiwell Plate 96 (Roche) using the SsoAdvanced Universal SYBR Green Supermix (BioRad, ...
-
bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed in 348-well plates on a LightCycler 480 instrument (Roche) using the Quantitect SYBR Green PCR Kit (Qiagen) ...
-
bioRxiv - Plant Biology 2020Quote: ... A 1 µg RNA was used to synthesize cDNA using a first-stand cDNA synthesis kit (Roche Diagnostics, Switzerland). The cDNA was normalized to 100 ng µl−1 and used for PCR analysis in a 10 µl reaction with gene-specific primers (5‘-CGTGGGATACCCTTACATGG and 5‘-CCCGATTATCTTCCACCAGA for ZmOMT1 ...
-
bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
-
bioRxiv - Immunology 2020Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA synthesis kit (Roche Applied Science) and the provided random hexamer primers for the mix preparation ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 1 µg of total RNA using the KAPA Stranded mRNA Hyperprep Kit (96rxn) (Roche, KK8581) according to manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2021Quote: PCR reactions were performed in triplicate in 384 well plates with SYBR Green (Roche) and specific primers ...
-
bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480 ...
-
bioRxiv - Pathology 2021Quote: Cells were sorted directly into a 96-well plate containing cell lysis buffer (Roche), and frozen immediately until random displacement amplification (RamDA ...
-
bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were removed from the magnetic block and 50 μl of elution buffer (Roche) was added to each well and mixed by pipetting up and down 10 times ...
-
bioRxiv - Neuroscience 2023Quote: ... The plate was inserted in the Roche LightCycler® 480 (Roche Life Science, Switzerland) and the cycles were defined as follows ...
-
bioRxiv - Bioengineering 2023Quote: ... qPCR was performed in 384-well plates using the LightCycler 480 Instrument II (Roche). Target gene expression change was calculated relative to non-targeting controls using the ddCt method.
-
bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the KAPA Hyper Prep kit (KAPA Biosystems) and NEXTflex ChIP-seq barcodes (Bio Scientific).
-
bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
-
bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
-
bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
-
bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed using ApopTag Red In Situ Apoptosis Detection Kit (S7100, MerckMillipore) combined with peroxidase (POD) coupled anti-DIG antibody (1/200, #11207733910, Roche) followed by tyramide-based amplification61.
-
bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
-
bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
-
bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
-
bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
-
bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
-
bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
-
bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
-
bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
-
bioRxiv - Cell Biology 2023Quote: ... First-strand cDNAs were obtained by reverse transcription of 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Roche), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
-
bioRxiv - Microbiology 2024Quote: ... Different dilutions of the enzyme preparation were assayed for 1 hour and analyzed with a luciferin-luciferase coupled reaction (76) using the CLSII-Bioluminiscent ATP Assay Kit (Roche).
-
bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems) quantified the prepared libraries ...