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Citations for Roche :
1 - 50 of 5855 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were first pre-incubated with 2.4G2 antibody (kindly provided by Louis Boon) for blocking Fc receptors and next stained for surface markers in PBS supplemented with 0.5% BSA (Roche) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and soluble FMS like Tyrosine kinase 1 (sFlt-1) on a Cobas e411 analyser (Roche Diagnostics, Mannheim, Germany).27 The serum hCG concentrations of the samples from the six individuals used in this study were compared against the respective LC-MS/MS derived values.
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bioRxiv - Immunology 2020Quote: ... 1% FCS and 12.5µg/ml DNAse (Roche) in IMDM (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... or HA peptide (Roche, 1 mg/mL) for detection by immunoblotting ...
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bioRxiv - Cancer Biology 2022Quote: ... progesterone receptor (PR) (790–4296, Roche) and human epidermal growth factor 2 (HER-2 ...
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bioRxiv - Neuroscience 2022Quote: ... Sugar-free HL3-like saline containing 30 units of Papain (Roche) was applied to the head capsule for 8 minutes to digest the brain’s glial sheath and facilitate removal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... HA peptide (Roche), Strep-Tactin XT Elution Buffer (IBA ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... HA peptide (400 μg/ml Roche) or cMyc peptide (200 μg/ml Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The Haemolymph-Like Salines (HL3 solutions) were prepared as described previously (Roche et al., 2002; Ataman et al., 2008). The list of all the solutions used is given below (Table 2).
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bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Second-stage digestion of SUMOylated peptides was performed with 1 μg of Endoproteinase Asp-N (Roche). Digestion was performed overnight ...
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bioRxiv - Immunology 2021Quote: BMDMs and THP-1 macrophage-like cells were lysed in ice-cold RIPA buffer supplemented with complete protease inhibitor and phosphatase inhibitor cocktails (Roche). Protein concentration was measured by the Pierce™ BCA Protein Assay Kit (Thermo Scientific-23227) ...
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bioRxiv - Cell Biology 2020Quote: ... peptide-N-glycosidase F (PNGase F) (Roche), neuraminidase (Roche) ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were transiently transfected with C5aR2-Venus and βarr2-Rluc8 constructs using XTG9 (Roche). 24 hours post transfection ...
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bioRxiv - Biochemistry 2019Quote: ... Bound biotin peptides were then detected by incubating with 1 U/ml of HRP-conjugated streptavidin (Roche) on ice for 45 min in PBS/0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the androgen receptor (AR) (RTU; rabbit, #760-4605, clone SP107; Roche, Basel, Switzerland). Staining was detected by the application of 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin and 20 IU human IL-2 (Roche Diagnostics, Mannheim, Germany) for 5d ...
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bioRxiv - Physiology 2019Quote: ... glucose-dependent insulinotropic peptide (GIP) and anorexigenic peptide PYY were simultaneously assayed on serum samples added with antiproteases (Complete EDTA Free, Roche, Meylan, France) using the Milliplex mouse metabolic hormone magnetic bead panel (MMHMAG-44K ...
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bioRxiv - Biochemistry 2021Quote: ... Peptide N-glycosidase F (PNGaseF) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... Human Fibronectin (Roche). Agar low melting point ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and ethanol precipitated with human Cot-1 DNA (Roche, Germany) and herring sperm DNA (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... human plasma fibronectin (Roche) was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Plant Biology 2022Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Physiology 2020Quote: ... Patch-clamp experiments on HCN1 and HCN2 were performed with transient transfection of 2 μg of either pCDNA3.1-HCN1 or pCDNA3.1-HCN2 (generously provided by Dr. Eric Accili) in HEK293 cells using Fugene6 (Roche, Basal, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The pcDNA3 constructs were used for transient transfection in HEK293 cells using an X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Plant Biology 2022Quote: ... and proteins were eluted by using 0.25 mg/ml HA peptide (Roche). HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... were performed essentially as described above but receptor solubilisation was performed in the presence of protease inhibitor cocktail (cOmpleteTM mini EDTA-free Protease Inhibitor cocktail (Roche)).
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...