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Citations for Roche :
301 - 350 of 2171 citations for Gap Junction Protein Alpha 9 GJA9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 µg of 330-BFP-CYP3A4-enhancer-R-sgRNA were transfected with X-tremeGENE 9 DNA transfection reagent (Roche 6365809001). After 3-5 days ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... were used for the purification steps and the libraries were amplified (9 amplification cycles) with the KAPA HiFi PCR Kit (Kapa Biosystems) in KAPA HiFi Fidelity Buffer (5x) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). Virus-containing supernatant was collected 48 hours after transfected and passed through a 0.45μm filter ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA expressing vectors along with lentiviral packaging vectors Delta-VPR and VSV-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001) ...
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bioRxiv - Neuroscience 2023Quote: ... prior to transfection with 2 μg pcDNA3.1 encoding N-terminally HA-tagged human 1N3R tau or 1N4R tau (14) using X-tremeGENE 9 (Roche Life Sciences), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
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bioRxiv - Genomics 2023Quote: ... sgRNA- or cDNA-expressing vectors and lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293T cells using XTremeGene 9 transfection reagent (Roche #636478700). The supernatant containing virus was collected 48 h after transfection and passed through a 0.45-µm filter ...
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bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection with full-length nV5-WT or nV5-P112L (0.8 μg DNA/well) was performed 24 h post-seeding using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Basel, Swityerland). 24 h post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Physiology 2021Quote: Pancreases were rapidly dissected after intra-ductal injection from 9-10 week-old male mice and islets were isolated by digesting the pancreas with collagenase P (Roche, Basel, Switzerland) and purified using a Ficoll gradient (Merck Biochrom ...
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bioRxiv - Developmental Biology 2020Quote: The testes from ten to twelve 19dpc embryos form 9 different mothers were collected and dissociated using collagenase/dispase (Roche, 1mg/ml), at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/-cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using X-tremeGENE 9 DNA transfection reagent (Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Immunology 2023Quote: The retroviral packaging cell line Platinum-E was co-transfected with pMXs-SOX2-IP or pMXs-IP using the DNA Transfection Reagent X-TremeGene 9 (ROCHE, Cat# 06365779001) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: 293T cells were co-transfected with plasmids encoding cognate heavy and light chains using the Xtreme Gene 9 DNA transfection reagent (Roche, Basel, Switzerland), then grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
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bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
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bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
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bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
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bioRxiv - Genomics 2020Quote: ... 2 g of DUX4 expression constructs were transfected into RD cells with 6 L of the X-treme GENE 9 DNA transfection reagent (cat. XTG9-RO, Roche Diagnostics, Indianapolis, USA) diluted in 100 L of Opti-MEM I (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We induced transient expression of the plasmids in 106 cells resuspended in 2 ml using the polymer X-tremeGENE 9 DNA Transfection Reagent (Roche Applied Sciences 06365787001) following manufacturer’s instructions (1 μg total DNA and 3 μl reagent in 100 μl FBS-free culture medium) ...
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bioRxiv - Bioengineering 2023Quote: ... was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene–specific primers (primer No.9–14) and a KAPA Biosystems kit (Roche, KAPA HiFi HotStart). The PCR conditions were as follows ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Zoology 2023Quote: Urine pH was assessed via pH meter (SevenEasy S20, Mettler Toledo, Columbus, OH, United States) and dipstick (Chemstrip® 9, Roche Diagnostics, Rotkreuz, Switzerland). The pH meter was calibrated before measurement using calibration buffer solutions with pH values of 4.00 ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G agarose beads (Roche #11719416001) pre-blocked with salmon sperm (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... together with protein A agarose (Roche) overnight at 4 ℃ ...