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Citations for Roche :
151 - 200 of 389 citations for Galectin 9 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... DNA was amplified by 9 cycles of PCR using the Kapa Hifi Uracil + DNA polymerase (KAPA Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification with 9 – 12 cycles using the KAPA HiFi HotStart Uracil + DNA Polymerase (Roche/KAPA Biosystems) was performed according to suggested protocols ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification with 9 – 12 cycles using the KAPA HiFi HotStart Uracil + DNA Polymerase (Roche/KAPA Biosystems) was performed according to suggested protocols ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were transfected into HeLa S3 cells (https://www.atcc.org/products/ccl-2.2) with X-tremeGENE 9 (Roche) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were then sequence-verified and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and single cells that were positive for GFP were sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche). For silencing experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... in 0.15 M hydrazine 10.9 mM EDTA pH 9 buffer and 80 U/ml lactate dehydrogenase (Roche) in 51 mM ammonium sulfate pH 6.5 buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2021Quote: The plasmids FUG-T2A-GFP-Cre and control GFP were purchased from Addgene #66691 and lentiviral particles were produced in HEK-293T cells using X-tremeGene 9 DNA Transfection Reagent (Roche). Upon ultracentrifugation concentrated virus particles were stored at −80°C and used to infect pancreatic ductal organoid cultures as previously described (Huch et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid and siRNA transfection into HeLa cells were carried out using X-tremeGENE 9 DNA transfection reagent (Roche) and Oligofectamine reagent (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Transfection targeting the AAVS1 locus was performed with X-TremeGene 9 following the manufacturer’s instructions (Roche, Indianapolis, IN).
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bioRxiv - Biochemistry 2020Quote: ... PFN1-KO or PFN2-KO) were split 1:3 and the next day transfected using XtremeGene 9 (Roche) with 5 μg V5-NAA80 (M23L ...
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... A pre-amplification step of 9 PCR cycles was performed using the Kapa HiFI Hoststart kit (Kapa Biosystems). The PCR product was purified using AmpureXP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Fig-ure 3D,F,J) or 48 h (for Figure 4H) using X-tremeGENE 9 DNA reagent (Roche) following the manufacturer’s instruc-tions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells were transiently transfected using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Cat. # XTG9-R0) with 6 µL of transfection reagent to 2 µg plasmid for 48 hr ...
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Cancer Biology 2022Quote: ... The mixture of plasmids (16.0 μg in 50 μL ddH2O) was added to 48 μL of X-tremegene 9 DNA transfection reagents (Roche) that pre-incubated for 5 min in 750 μL OptiMEM (Gibco) ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C and 5% CO2 were seeded onto ibidi μ-slides at 30,00 cells/well and transfected with TDP-43RRM-GFP reporter constructs (300 ng) using X-tremeGENE 9 (Roche). After 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... allowed to adhere overnight and transfected with the indicated reporter constructs (300 ng/well) using X-tremeGENE 9 (Roche). 24 later ...
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bioRxiv - Cell Biology 2019Quote: ... allowed to adhere overnight and transfected with the indicated reporter constructs (500 ng/well) using X-tremeGENE 9 (Roche). 24 hours after transfection ...
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bioRxiv - Biochemistry 2019Quote: ... The Bacmid DNA was purified and transfected into SF9 insect cells with Xtreme gene 9 DNA transfection reagent (Roche). The first virus generation ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 μg of the above vector with 6 μg pCMV8.9 and pCMV-VSVG packaging vectors were transfected into the 293 packaging cell line using X-tremeGENE 9 (Roche), and the resulting viral supernatants were harvested as previously described13 ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids and ssDNA were transfected into hESC_H1 (WiCell) of 70-85% confluency using the XtremeGENE 9 transfection reagent (Roche). After 48 hours ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with mammalian construct DNA according to manufacturer instructions with X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Cancer Biology 2021Quote: U2OS cells stably expressing T192-Flag-FKBP were grown on coverslips and transiently transfected with either MYOF-FRB* or MYOFΔC2-FRB* using X-tremegene 9 DNA Transfection Reagent (Roche). After 24h ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 200 ng of plasmids for 48 h using X-tremeGENE 9 DNA reagent (Roche, Fig. 2A), or electroporated with 2 μg of plasmid using Lonza’s nucleofection protocol (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... S3C) or 120 ng of plasmid for 24h (Fig. 3E) for 24h using X- tremeGENE 9 DNA reagent (Roche).
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bioRxiv - Cancer Biology 2022Quote: ... DF1 cells were transfected with the indicated RCAS plasmid using X-tremeGENE 9 DNA transfection reagent (Roche, Catalog# 06365809001) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 100mg of frontal cortex tissue (Brodmann area 8/9) were thawed and homogenized in 500μl of PBS with protease inhibitor (Roche) by 30 up and down strokes in a glass Dounce homogenizer ...
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bioRxiv - Microbiology 2021Quote: Virus stocks were generated by transfection of 293T cells with each plasmid clone of HSIV-vif using Fugene 6 or X-tremeGENE 9 DNA transfection reagent according to the manufacturer’s protocol (Roche). Infectious titers were determined by limiting dilution infection analysis using TZM-bl indicator cells and the amount of virus in supernatants was measured by HIV-1 p24gag antigen ELISA (Advanced Bioscience Laboratories).
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bioRxiv - Biophysics 2019Quote: ... 4 million cells were seeded in a 10 cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 cells were transfected with FAM-labelled hairpins using X-tremeGENE 9 DNA Transfection Reagent (Roche, USA; 1.0 µL). After 16 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid minigenes were transfected 24 h after cell seeding using X-tremeGENE 9 DNA Transfection Reagent (Roche; Mannheim, Germany). HepG2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2018) were combined with a transgenic construct (9 µg) and co-transfected using X-tremeGene HP (Roche Applied Science) at a 1:3 ratio of DNA to the transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated by co-transfecting HEK293T cells with the lentiviral construct pCMV-dR8.91 (Δ8.9) plasmid and the pMDG envelope-expressing plasmid using X-tremeGENE 9 DNA Transfection Reagent (Roche, 6365779001). Viral supernatant was collected within 24-48 h post-transfection for subsequent infection of the target cells.
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bioRxiv - Neuroscience 2023Quote: ... tissue sections (3-5μm) from frontal cortex (BA 8/9) using the Nexes station automated system (Ventana Medical System Inc., Roche). Deparaffinised and rehydrated sections were pretreated and blocked ...
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bioRxiv - Physiology 2019Quote: ... Serial dilutions of human genomic DNA (Roche) (final concentrations from 0.5 ng/mL to 5000 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...