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Citations for Roche :
251 - 300 of 596 citations for Frizzled 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 mM Tris-HCl pH 8) supplemented with protease and phosphatase inhibitor cocktail (Roche Molecular Biochemicals), and at 4 °C with gentle shaking/tumbling for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 mM Tris-HCl pH 8 supplemented with phosphatase and protease inhibitor cocktail tablets (Roche Diagnostics)) ...
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bioRxiv - Neuroscience 2021Quote: ... 8 μL of each sample E3 was used directly for PCR as described above (KAPA Biosystems). Embryos were kept in 48-well plates until genotyping was achieved.
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Microbiology 2020Quote: ... pH 8) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 20 mL; Roche, Mannheim ...
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bioRxiv - Cell Biology 2021Quote: ... overnight cultures were diluted to OD600 = 0.1 and treated with 8 mM DTT (Roche, Mannheim, Germany) for up to 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was amplified using SV40_NLS_seq_f and SV40_NLS_seq_r (Supplementary Table 8) primers using Kapa Hifi (Kapa Biosystems KK2602) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The pellet was resuspended in 8 mL of polysome buffer containing 1x Protease inhibitor Cocktail (Roche) and 1 mM PMSF ...
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bioRxiv - Microbiology 2019Quote: ... 80mM EDTA pH 8 and complete EDTA-free protease inhibitor cocktail according to instructions (Roche, CH)) ...
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bioRxiv - Immunology 2021Quote: ... 50 mM Tris-Cl (pH 8) supplemented with Complete Protease Inhibitor Cocktail tablet (Roche, Mannheim, Germany) and 1x Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 μL of extraction solution (8 M Urea, 25 mM NH4HCO3, Protease inhibitor (cOmplete tab, Roche Switzerland ...
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bioRxiv - Developmental Biology 2023Quote: ... Each sample was lyzed in 200 mM HEPES buffer (pH 8) containing PhosSTOP phosphatase inhibitors (Roche), phosphatase inhibitor cocktail2 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... pH 8) containing 10 mM Imidazole and supplemented with Protease Inhibitor Mixture (Complete, Roche Applied Science) for lysis at 4 °C by sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Triton-X 100 at pH 8) supplemented with EDTA-free complete protease inhibitors (Roche, 56619600). Lysozyme was added to 1 mg/mL and lysate incubated for 20 min on ice ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... + 0,4 g/l of human albumin (Vialebex) with Liberase TL (Roche) 150 ug/ml and DNase 1 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
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bioRxiv - Genomics 2019Quote: ... using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: Human genomic DNA of high molecular weight (Roche Diagnostics, Basel, Switzerland) were processed in a single replicate per DNA extraction protocol (Table 1) ...
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bioRxiv - Neuroscience 2020Quote: DNA from the patient and his parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissues were lysed with 8 M urea in PBS supplemented with protease and phosphatase inhibitor cocktail (Roche) at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was probed with a 1:20000 dilution of mouse anti-GFP antibody (JL-8, Roche) or mouse anti-alpha-tubulin antibody (B-511 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris pH 8) containing protease inhibitors (Roche mini EDTA-free, 1 tablet in 10 ml) and phosphatase inhibitors (2mM Sodium Ortho-Vanadate ...
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bioRxiv - Developmental Biology 2021Quote: ... we lysed samples in 8 μl of lysis buffer PBND containing 0.1 mg/ml Proteinase K (Roche) (Scavizzi et al. ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml ice-cold 8 % (v/v) paraformaldehyde solution in vPBS containing EDTA-free protease inhibitor (Roche) or ice-cold 8% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human samples using TriPure Isolation Reagent (Roche). RNA integrity (RIN ...
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bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... eosin and antibodies for human keratins AE1/AE3 (760-2135; Roche diagnostics). Metastasis quantification was reached through a review of 17 digitalized slides ...
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... brain from transgenic mice expressing different PrP deletion mutants was homogenized in 10 volumes of lysis buffer (50 mM Tris-HCl pH 8, 0.5% Na deoxycholate, and 0.5% Igepal, protease inhibitors (complete Mini, Roche) using TissueLyser LT for 5 min for 2 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in 450 μl lysis buffer (20 mM Tris pH 7.5, 0.5 mM EDTA pH 8, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 10 mM NEM, Roche cOmplete protease inhibitor catalog # 11836153001) ...
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bioRxiv - Microbiology 2020Quote: RNA of high quality (RINe>8) was prepared for mRNA-sequencing using the KAPA mRNA HyperPrep kit (Roche). Libraries were quantified using a High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... and chloride [Cl-] concentrations were determined using an Ion Selective Electrode (Hitachi Modular P+ISE. Roche 8 Diagnostic). Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by 8 min of perfusion with digestion buffer (4mg/mL trypsin, 5mg/mL liberase (Roche) and 0.3mM CaCl2) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were lysed with Cell Lysis Buffer (10mM Tris-Cl pH 8, 10mM NaCl, 0.5% NP40, 1xPIC (Roche)) followed by mechanical shearing for 10minutes on ice ...
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bioRxiv - Molecular Biology 2022Quote: Pelleted cells were lysed in 500 μl 8 M urea (pH 8.0) with 0.2% Rapidgest (Waters) and protease inhibitors (Roche) using three 20-s sonication pulses in a VialTweeter (DrHielscher) ...