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Citations for Roche :
351 - 400 of 4892 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Eluted materials were transferred to new tubes and treated with RNase A (100 μg/ml; Roche) for 2.5 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg/ml AEBSF [4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride] and protease inhibitors cocktail (Complete, Roche). Cells were cracked by multiple passages through a microfluidizer system using a pressure of 18’000 psi ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were cultured in RPMI + IL-2 (final concentration 100 U/mL, Roche, 10799068001), IL-7 (final conc ...
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bioRxiv - Plant Biology 2023Quote: ... except that 2 g of fresh weight whole seedlings were harvested for each genotype (3 biological replicates each) and 1.5 ug of anti-HA 3F10 (Roche) antibody were used for immunoprecipitation ...
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bioRxiv - Microbiology 2022Quote: ... 95°C), annealing (10 sec, 58°C) and elongation (10 sec, 72°C) performed on a LyghtCycler 96 Instrument (Roche). MIC14 and MIC15 transcripts were amplified with the primer pairs P64/P65 and P66/P67 ...
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bioRxiv - Immunology 2022Quote: ... pursued by 95°C for 3 min and then 45 cycles at 95°C for 15 sec and 58°C for 30 seconds using a LightCycler-480 Real-Time PCR system (Roche). The primers and the probes were designed against the E gene17.
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bioRxiv - Microbiology 2021Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Microbiology 2023Quote: ... followed by 95 °C for 2 min and then 45 cycles of 95 °C for 15 s and 60 °C for 30 s using a Light Cycler 480 (Roche).
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bioRxiv - Bioengineering 2022Quote: ... PMSF (MP Biomedical) and protease inhibitor tablets (1 tablet/100 mL, cOmplete Mini EDTA-Free, Roche Diagnostics), 5ml / g wet weight of pellet ...
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bioRxiv - Genetics 2019Quote: ... 100 μl buffer and 200 μl H2O) and supplemented with 0.5 mg/ml proteinase K (03115887001, Roche). Cells were lysed at 55°C for 2 hours while shaking at 350 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% Triton X-100) + 0.5 mg/ml BSA + protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail; Roche) and the reaction allowed to proceed for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... 1% Triton X-100 and 0.1% SDS) with protease inhibitor (Roche/1 tablet in 10 mL RIPA). Sample homogenates were centrifuged for 5 min at 4°C at 13,000 G ...
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bioRxiv - Immunology 2020Quote: Harvested lymph nodes were subjected to digestion at 37°C in DNase I (100 mg/mL, Roche), Collagenase P (200 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... FL fragments were treated for 20 minutes at 37°C with 100 μg/mL of Liberase (Roche) to obtain a cell suspension ...
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bioRxiv - Immunology 2022Quote: ... The excised tumours were cut into small pieces and digested with 100 μg/ml DNase I (Roche) and 200 U/ml Collagenase (Worthington ...
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bioRxiv - Microbiology 2022Quote: ... The matrix was gently washed in PBS thrice and incubated with 100 mg/mL RNAse A (Roche) and 10 IU/mL DNAse (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Detection was done by incubation with 1 ml of detection buffer containing 100 μL CDP-Star (Roche) and exposure in a Gel Doc.
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bioRxiv - Genomics 2022Quote: ... The extra-viral nucleic acids were then degraded with 100 U/mL DNase (Roche, Cat. No. 10104159001) for 1 h on the bench top ...
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bioRxiv - Genetics 2019Quote: ... 1 ug purified digested plasmid was used as template for SP6 or T7 transcription using the DIG-RNA labeling kit (Roche). Following probe transcription ...
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bioRxiv - Genomics 2021Quote: ... Early passage cells were seeded at 25% confluency in six-well plates and transfected with 2 ug of expression vector using Fugene reagent (Roche). Cells were harvested and seeded into 100 mm dishes after 48 h of transfection ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were boiled 15 min at 95°C to gelatinize the starch and hydrolyzed with α-amylase and amyloglucosidase (Roche) to release the glucose blocks ...
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bioRxiv - Microbiology 2022Quote: ... followed by 40 cycles of amplification (95°C for 15 sec, 60°C for 30 sec) (Roche LightCycler 96, Roche Diagnostics). For absolute quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 50-60 min at 37οC with 20 µM EdU and 100 ng/mL colcemid (Roche). Following metaphase arrest ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 % Triton X-100) with 0.5 mg/ml Lysozyme and 50 μg protease inhibitor cocktail tablet (Roche Biochemicals) followed by sonication on ice ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells were maintained in Myelocult medium supplemented with penicillin/streptomycin and 100 U/ml IL-2 (Roche). CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... water was replaced with 100 μl of a 2 μg/ml horseradish peroxidase (type II; Roche, Rotkreuz, Switzerland) and 5 μM L-012 (WAKO Chemicals ...
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bioRxiv - Genetics 2020Quote: ... with fetal bovine serum (FBS, 10% v/v, Biowest) and Penicillin/Streptomycin (100 U/mL, Roche Diagnostics GmbH). Cells were cultured on 10 cm sterile plates in 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with proteinase K (1 mg/mL in 100 mM Tris-HCl, pH 8.0, 50 mM EDTA; Roche) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Neuroscience 2019Quote: ... The PVDF membranes were treated and immersed into the rabbit anti-mouse PSD-95 monoclonal antibody (1:1000, Roche Group, Nutley, USA) to incubate at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... pursued by 95°C for 3 minutes and then 45 cycles at 95°C for 15 seconds and 58°C for 30 seconds using a LightCycler 480 Real-Time PCR system (Roche, Rotkreuz, Switzerland). The primers and the probes were designed against the E gene (20).
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bioRxiv - Neuroscience 2023Quote: ... in Tris-EDTA buffer pH 7.8 at 95°C for 64 min in standard cell condition solution one (CC1) using a Ventana Discovery ULTRA (Roche Indianapolis IN). Blocking was then performed in an inhibitor solution for 12 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton X-100 and 0.1% SDS) with a protease inhibitor cocktail (Roche/1 tablet in 10 mL RIPA). Homogenates were centrifuged for 5 min at 4°C at 13,000 x G ...
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bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). FRMP binding assay was performed by incubating biotinylated transcripts (250 ng ...
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bioRxiv - Microbiology 2021Quote: Collected cell pellets were lyzed in a lysis buffer (40 mg/ml SDS, 100 mM Tris-HCl pH 8.6, 10 mM EDTA and Complete protease inhibitors (Roche)) and sonicated 3 times for 30 s at 40% amplitude ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were then resuspended in 100 µl TE plus 0.25% SDS supplemented with proteinase K (500 µg/ml, Roche) and incubated overnight at 60°C ...
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bioRxiv - Cell Biology 2020Quote: ... cell nuclei were counterstained with 100 ng/ml of DAPI for 10 min at room temperature (Roche Applied Science). Slides were mounted on coverslips using Fluoromount mounting medium (DAKO) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized with 0.5% TritonX- 100 for 15 minutes at room temperature and incubated with 1mg ml−1 DNase I (Roche) for one hour at 37 °C followed by incubating with anti-BrdU (BU20A ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down again and the pellet resuspended in 1.25 ml cold lysis buffer (PBS containing 1% TX-100, 1 mM NEM, and Complete Protease Inhibitor from Roche). Cells were lysed for 30 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Biophysics 2021Quote: ... Cells were resuspended in 100 ml of lysis buffer (20 mM sodium phosphate pH 7.4, 500 mM NaCl with DNase I (Roche), 0.5 mM MgCl2 and 1 mM PMSF) ...
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... They were lysed in polysome lysis buffer containing 100 μg/mL cycloheximide and protease cocktail inhibitor EDTA-free (Roche)) ...
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Pelleted cells were resuspended in 20-30 mL Wash Buffer (150 mM NaCl, 100 mM Tris, pH 8.0) and a cOmplete protease inhibitor tablet (Roche) was added ...