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Citations for Roche :
501 - 550 of 5091 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Primers and probes were designed using the predicted genes sequences generated in the RNA-seq data analysis and the Assay Design Center from Roche, selecting “Other organism” (https://lifescience.roche.com/en_gb/brands/universal-probe-library.html) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then placed on ice and were lysed in lysis medium (basal assay medium, 1x protease inhibitor cocktail complete with EDTA (Roche), 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were analyzed according to the manufacturer’s guidelines using the Elecsys Anti-SARS-CoV-2 assay on the Roche cobas e602 platform (Roche Diagnostics). A cut-off index (COI ...
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bioRxiv - Neuroscience 2021Quote: ... Relative quantitative PCR assays were carried out using a LightCycler 480 and the SYBR Green I Master mix (Roche LifeScience), with Act5C as internal control for normalization of mRNA levels ...
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bioRxiv - Neuroscience 2021Quote: ... for qRT-PCR were intron-spanning to avoid amplification of non-digested genomic DNA fragments and were designed by online Universal ProbeLibrary Assay Design Center (Roche). Standard housekeeping genes (elongation factor 1 ...
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bioRxiv - Immunology 2021Quote: ... 180 and 365 using Roche Elecsys® Anti-HBs antibody assay on an Elecsys® 2010 analyzer (Roche, Basel, Switzerland). An anti-HBs titer above 10 IU/ml was considered protective (Keating and Noble ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed in assay buffer (1% NP-40) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNAs were used for the quantitative PCR assay on a LightCycler 480 Real-Time PCR System using the SYBR Green I Master mix (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The primers detecting the different CEACAMs mRNAs and their splice variants were designed by the Universal ProbeLibrary Assay Design Center (Roche) and are listed in Supplementary Table 1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Worms were harvested from either the mesenteries or liver of mice, then homogenized in assay buffer (PBST, supplemented with HEPES 10mM and protease inhibitor (Roche cOmplete Mini EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Developmental Biology 2019Quote: ... The qRT-PCR assay was performed in triplicate with SYBR Green I Master reagent and the Light Cycler Nano system (Roche). Actin was used as the internal control for normalization ...
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bioRxiv - Pathology 2019Quote: Cells were washed 2 × ice-cold PBS and scraped on ice into 200 μl of ice-cold radioimmunoprecipitation assay buffer supplemented with protease (cOmplete mini, ROCHE) and phosphatase inhibitors (1 mM Sodium Orthoranadate ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCRs were performed in 96-well plates in a PCR thermal cycler (Applied Biosciences 7900HT) using a 2x FastStart TagMan Probe Mastermix assay (Roche) with a probe concentration of 100 nm and a primer concentration of 200 nm with the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were washed once with ice-cold phosphate buffered saline (PBS) and collected in 150 µl radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors (cOmplete, Roche) 24 hours after transfections ...
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bioRxiv - Cancer Biology 2019Quote: Real-time PCR analysis of cDNA samples was performed with specific primers and probes designed by using Assay Design Center (Roche). Primers and probes used here are available upon request ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
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bioRxiv - Pathology 2021Quote: ... Quantitative PCR (qPCR) assay was performed by using the SYBR qPCR Master Mix (Vazyme) on Roche Lightcycle480 II Sequence Detection System (Roche). Gene expression levels were normalized by Actb ...
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bioRxiv - Physiology 2020Quote: ... Primers for genes of interest were designed by the Universal Probe Library Assay Design Centre (Roche Applied Science, Penzberg, Germany) and purchased from Eurofins MWG Operon (Ebersberg ...
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bioRxiv - Immunology 2021Quote: ... Total plasma cholesterol and triglyceride concentrations were quantified in murine plasma and liver homogenates using colorimetric assays (CHOD-PAP and TAG-PAP Roche) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... Semi-confluent astrocyte cultures were lysed in ice cold radio-immunoprecipitation assay lysis buffer with protease and phosphatase inhibitors (Roche), and 10 µg protein was separated by electrophoresis on 4-12% polyacrylamide gels followed by transfer to nitrocellulose membranes (all gels/reagents from Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... At the completion of the assay islets were lysed in RIPA buffer (Pierce) containing 1 mM PMSF and EDTA-free protease inhibitor cocktail (Roche)) at 95°C for 5 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Glucose was then quantified by measuring NADH production using an assay based on hexokinase and glucose-6-phosphate dehydrogenase (Roche). Starch content was calculated in glucose equivalents.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time reverse transcription PCR assays were conducted in 96-well plates using the LightCycler 480 Instrument (Roche, Wilmington, MA). Levels of mRNA expression were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Microbiology 2022Quote: ... Blood-stage parasite proteins were isolated by lysis in 50-200μL of radioimmunoprecipitation assay (RIPA) buffer and washed in PBS with cOmplete™ protease inhibitor cocktail (Roche). Samples were resuspended in 5x SDS-Laemmli buffer and denatured at 95°C.
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bioRxiv - Cell Biology 2023Quote: ... Transcript levels were quantified by quantitative real-time PCR (qRT-PCR) assays using the appropriate probes from the Universal Probe Library (UPL, Roche). The website service (https://lifescience.roche.com/en_tw/brands/universal-probe-library.html ...
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bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were lysed on ice with a cell scraper and radioimmunoprecipitation assay lysis buffer (Pierce) supplemented with protease inhibitor tablets (Roche) and a phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Physiology 2023Quote: ... Each RT-qPCR assay was deposited in triplicates on a FrameStar® 384-well skirted qPCR plate (Roche Diagnostics, Switzerland). The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then lysed in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP phosphatase and CompleteMini protease inhibitors (Roche, Switzerland) for protein extraction ...
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bioRxiv - Plant Biology 2023Quote: ... Primers and Taqman probes were designed using Probe Finder software in the Universal Probe Library (UPL) Assay Design Center (Roche). All PCR reactions were performed using a StepOne System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: DNA extracted from FFPE lung tissue samples was sequenced through an orthogonal method by targeted enrichment (Roche Avenio Targeted Assay) and sequencing (NextSeq 500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridization of the DIG-telomere probe and chemiluminescent detection was performed according to the protocol from the TeloTAGGG Telomere Length Assay (Roche). Briefly ...
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bioRxiv - Microbiology 2023Quote: Poly dA/oligo dT substrate for Pol-Prim stimulation assays was created by mixing 100 nanomoles of poly [dA] (average length 250-500nt) (Roche) with 5 nanomoles of oligo [dT] (average length 18nt ...
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bioRxiv - Plant Biology 2023Quote: ... Quality and quantity of sequencing libraries were evaluated and quantified using Bioanalyzer and KAPA Library Quantification assay (Roche, Basel Switzerland). Paired-end sequencing (2 × 75 bp ...
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bioRxiv - Genomics 2023Quote: ... KASPar genotyping assays were carried out with a single end-point measure on a Q-PCR Light Cycler 480 (Roche) using the Agencourt® DNAdvance kit (Beckman) ...
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bioRxiv - Immunology 2023Quote: Cell viability upon transduction of tBID death reporter was assessed in 96-well plates through WST-1 assay (Roche, Switzerland) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease (SERVA) and phosphatase inhibitors (Roche). The protein concentration was determined using a Bicinchoninic Acid Protein (BCA ...
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bioRxiv - Cancer Biology 2021Quote: ... 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cancer Biology 2021Quote: ... The exome captured by the SeqCap wash kit and SeqCap pure capture bead kit (both from Roche). The Illumina Novaseq platform was used to sequence 150 bases (paired-end sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Plant Biology 2022Quote: ... The EMSA was carried out utilizing the manufacturer’s instructions (DIG-gel shift kit 2nd generation kit, Roche).
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bioRxiv - Developmental Biology 2021Quote: ... Colorimetric in situ hybridization assays used sense and anti-sense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland), following a published procedure (Sharma et al. ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Biochemistry 2020Quote: A fluorescence-based thermal stability assay (differential scanning fluorimetry, DSF) (51) was performed using a LightCycler®480 II instrument (Roche). 20-μl samples were analyzed in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... were added and after an additional 6 hours BrdU was added and a cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences).
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bioRxiv - Microbiology 2019Quote: ... Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Transduced MEFs were seeded in triplicate in a 96 well flat bottom tissue culture plate and WST-1 assay (Roche, Switzerland) performed as per manufacturer’s protocol at day 0 ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
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bioRxiv - Epidemiology 2019Quote: ... N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitive cardiac troponin T (hs-TnT) were determined on the same analyzer by immunoturbidimetry assays (Roche Diagnostics). Lipoprotein A-I (containing apoA-I but not apoA-II ...