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Citations for Roche :
451 - 500 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Pelleted cells were resuspended in 20-30 mL Wash Buffer (150 mM NaCl, 100 mM Tris, pH 8.0) and a cOmplete protease inhibitor tablet (Roche) was added ...
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bioRxiv - Genomics 2023Quote: ... synovial biopsies were minced and digested in a mixed enzymatic-mechanical dissociation including 100 μg/ml Liberase TL (Roche) and 100 μg/ml DNAse I (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining cortical tissue was minced and transferred to 10 mL HBSS containing Collagenase A (Roche, 50-100-3278) at 200 units/ml ...
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bioRxiv - Immunology 2023Quote: ... were lysed in 1 mL lysis buffer (1% IGEPAL-CA 630, 300 mM NaCl, 100 mM Tris pH 8.0, 1X Roche cOmplete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
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bioRxiv - Biochemistry 2020Quote: ... 20 ng/μl creatine kinase (Roche Applied Science). Chd1-containing reactions were performed in 26.6 mM Na·HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 45 ng/μl Proteinase K (Roche, Basel, Switzerland) was added followed by a second incubation step at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... 20 ng/μl creatine kinase (Roche Applied Science). Remodeling reactions were started by adding 10 μL SGD chromatin corresponding to ~ 1 μg DNA assembled into nucleosomes and terminated by adding 0.8 Units apyrase (NEB ...
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Development of a novel allograft model of prostate cancer: a new tool to inform clinical translationbioRxiv - Cancer Biology 2020Quote: ... and phenol-chloroform extraction and cDNA synthesised using first strand cDNA (Roche) according to the respective manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed employing the High Pure RNA Isolation Kit (Roche), with an additional DNA digestion step that involved the addition of DNAse (Ambion™ ...
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bioRxiv - Cancer Biology 2023Quote: ... Locations of interest on the slides were chosen based on slides from the same blocks stained with the markers P504s (clone SP116, Roche, #8035130001) and p63 (clone 4A4 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression of melanocytic markers and PD-L1 was determined using intron-spanning primers with SYBR FAST qPCR master mix (Kapa Biosystems). qRT-PCR was performed with the following primers ...
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bioRxiv - Microbiology 2021Quote: Viral RNA of all VSV-spike pseudovirus particles were extracted from 200ul supernatant using the High Pure Viral RNA Kit (Roche, Cat. No. 11858882001) following the supplier’s manual ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... and then the detection reaction was carried out in a buffer containing 3.5 µl/ml of of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Roche, Bâle, Switzerland). The reaction was stopped with 50 mM EDTA in PBS and embryos were washed in PBSTw ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was moved to a new 1.5 ml Eppendorf tube and 600 μl of dilution buffer and 100 μl protease inhibitor cocktail (Roche #11836153001) were added ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... dried and solubilized in 0.2 ml Hepes (100 mM) buffer containing cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) (1 tablet/20 ml of buffer solution) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 mM KCl, 10 mM MgCl2, 1% Triton-X100, 100 ug/ml cycloheximide, 1 mM DTT, complete EDTA-free protease inhibitors – Roche) plus 500 uL acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... Coli tRNA at RT for 10 minutes and resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). The assay was then performed in presence of 250 ng of biotinylated transcript ...
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bioRxiv - Physiology 2021Quote: ... and 400 ml of sample was placed in a 2-l wide-mouthed Erlenmeyer culture flask with 100 ml of freshly prepared blendzyme (Roche Liberase TM ...
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bioRxiv - Microbiology 2022Quote: PPs were excised from the SI and digested (37 °C, 45 min) using 100 µg/ml liberase TH/DNase (Roche) in RPMI containing 5 % FCS ...
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bioRxiv - Bioengineering 2020Quote: The cell-free reaction mixture for the KGK10 strain is identical to that used for the T7 SHuffle® with the addition of 100 μg/mL DsbC (GeneFrontier) and 3.33 Units/uL T7 RNAP (Roche). Previously prepared KGK10 extract fermented and processed by the Swartz lab was tested alongside the in-house KGK10 extract prepared by shake flasks.
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bioRxiv - Neuroscience 2020Quote: ... To lyse the cells medium was removed and the well was washed with 2 ml ice cold PBS before 100 µl lysis buffer was applied (RIPA buffer supplemented with PhosSTOP (Roche) and protease inhibitors (cOmplete Mini ...
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bioRxiv - Neuroscience 2020Quote: ... 18 µl cleared cell lysate or brain homogenates (20-100 µg based on Tau aggregate content) were incubated with 2 µl 1 mg / ml pronase (Roche) at 37° C for one hour ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM imidazole and 10% glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Immunology 2019Quote: ... memory B cells were plated at 6 B cells in 55 μl per well into 96 × 384-well plates in B cell media supplemented with 100 U ml−1 IL-2 (Roche), 50 ng ml−1 IL-21 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... medium was removed and the well was washed with 2 ml ice cold PBS before 100 μl lysis buffer were applied (RIPA buffer supplemented with PhosSTOP; Roche) and protease inhibitors (complete Mini ...
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bioRxiv - Microbiology 2021Quote: ... At an OD600 of 5-6 the cells were harvested at 11.325 g and 4 °C for 15 min and cell pellet of 100 ml cell culture was resuspended in 25 ml buffer A (100 mM Tris-HCl, pH 8.0) with cOmplete™ Proteinase inhibitor (Roche). Cell disruption was performed using the French Press cell with a pressure of 172 mPA for five passages ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 20 mM KCl, 2 mM MgCl2, 1% TX-100, 40U Ribolock ml-2 and protease inhibitor cocktail, Roche) followed by clearing at 17 000 × g for 10 min at +4C° ...
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bioRxiv - Immunology 2023Quote: ... Washed small intestine pieces were then finely chopped and incubated in serum-free medium added with 100 μg/ml Liberase TL (Roche) and 50 μg/ml DNAse I (Roche ...
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bioRxiv - Immunology 2023Quote: ... Stored synovial tissue samples were then thawed and disaggregated into single-cell suspensions by mincing and digesting with 100 µg/mL LiberaseTL (Roche) and 100 µg/mL DNaseI (Roche ...
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bioRxiv - Immunology 2022Quote: ... Excised tumors were cut into small pieces and digested at 37°C for 30 min with 100 μg/ml DNase I (Roche) and 200 U/ml Collagenase (Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... The microsome fraction was solubilized in 1% Triton X-100 in PBS (10 mg microsome per ml) with protease inhibitor cocktail (complete mini, Roche) by rocking at 4°C for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... each supplemented with protease and phosphatase inhibitors (2X cOmplete, EDTA-free, Protease Inhibitor Cocktail [Roche]; 1X PhosSTOP Phosphatase Inhibitor [Roche]; 100 µg/mL Pefabloc SC [Roche]), while incubating on ice for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: Brains were dounce homogenized in RPMI (1X) + 10% Cosmic Calf Serum (Hyclone) and digested with 100 mg/mL collagenase/dispase (Roche) and 1 mg/mL DNase I (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted in the extraction buffer containing protease inhibitor cocktail (Roche) as described above ...
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bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction was performed manually using the High Pure RNA isolation kit (Roche) with an on-column DNase treatment according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... phenol-chloroform extraction and ethanol precipitation in presence of 20 µg glycogen (Roche). Input material (1.5% ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated using the KAPA Express Extraction protocol (KAPA Biosystems, # KB7101). Briefly ...
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bioRxiv - Genetics 2021Quote: ... 5 ng of cDNA on a LightCycler96 system (Roche) according to the manufacturer’s instructions ...