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Citations for Roche :
1 - 50 of 1848 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: c-fos DIG antisense riboprobe was in vitro transcribed (DIG RNA Labeling Mix, Roche) from an EcoRI fragment of c-fos cDNA (Dr Inder Verma ...
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bioRxiv - Molecular Biology 2022Quote: ... and hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low stringency washes (washing twice with 2× SSC/0.1% SDS at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... at 4°C with alkaline phosphate anti-DIG (1:2000, Roche). For chromogenic revelation ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Molecular Biology 2022Quote: ... UV cross-linked (1200J/m2) and hybridized with a DIG-labelled (GCCTAA)4 probe at 37°C using DIG Easy Hyb (Roche) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG; Integrated DNA Technologies, Coralville, IA; Table S1) overnight at 42°C DIG Easy Hyb™ buffer (Roche). Blots were washed in 2X saline-sodium citrate (SSC ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated overnight at 4°C with anti-DIG antibody (11093274910, Roche) at 1:5000 in blocking buffer ...
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bioRxiv - Genetics 2020Quote: ... The DIG probe was hybridized to the membrane (at 44°C; using Roche DIG Easy Hyb Granules for hybridization buffer ...
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bioRxiv - Biophysics 2023Quote: ... 1a) in a 27°C water bath for 15 minutes and then quenched with 0.5 mM PMSF (Roche). Samples were analyzed on a Coomassie stained SDS-PAGE PAGE gel ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked using DIG Easy-Hyb for 1 hour at 42°C (Roche, Cat#11093274910). Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4 °C in MABB with anti-DIG POD (Roche) at a 1:1,000 dilution ...
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bioRxiv - Genomics 2024Quote: ... and incubated overnight at 4°C with Anti-Dig-AP antibody (Roche, 1:5000) in 1% lamb serum ...
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bioRxiv - Plant Biology 2023Quote: ... Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche) and then hybridized overnight at 50°C in same buffer using a denatured probe ...
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bioRxiv - Microbiology 2020Quote: ... DIG Washing and DIG Blocking Buffers were prepared using the DIG Wash and Block Set (Roche, USA). The blot was washed twice with Washing Buffer for 10 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... DIG-positive cells were visualized with anti-DIG antibody (Roche Applied Science ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DIG rUTP (Roche) and Fluorescein rUTP (Roche ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The membrane was hybridised overnight at 42 °C with DIG Easy Hyb (Roche®, 11603558001). The DNA was detected with anti-digoxigenin-Alkaline Phosphatase antibody (Roche® ...
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bioRxiv - Developmental Biology 2022Quote: ... cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche, 11 175 025 910). Images were taken on an SPE Leica confocal microscope and processed using FIJI and Adobe Photoshop programs.
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bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in horseradish peroxidase-coupled anti-DIG antiserum (11207733910, Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was pre-hybridized and hybridized at 50°C in DIG eazy Hyb (Roche). Two double washing steps were realized using 0.1% SDS ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxygenin (DIG) RNA labelling was performed using DIG-labelling kit (Roche) following instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were hybridized with 30μg/mL of Digoxigenin (DIG) conjugated telomere probe (CCCTAACCCTAACCCTAACCCTAA-DIG, IDT) in DIG Easy Hyb buffer (Roche) overnight at 42°C ...
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bioRxiv - Neuroscience 2023Quote: ... PH 7.5)) and then incubated overnight at 4 °C in anti-DIG POD antibody (Roche, 11207733910) diluted 1:300 in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... DIG-labelled probe prepared using PCR DIG Probe Synthesis Kit (#11636090910, Roche) was added and the membranes incubated for over 18 h ...
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bioRxiv - Neuroscience 2021Quote: ... DIG-labeled RNA probes were then synthesized (DIG RNA Labeling Mix, Roche) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... DIG labelled probes were synthesised using a DIG RNA labelling mix (Roche). DNP labelled probed were synthesised using a similar process but with the DIG labelling mix replaced by a 20x NTP stock solution (20mM each ATP ...
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bioRxiv - Developmental Biology 2024Quote: ... DIG-labeled probes were prepared using a DIG RNA labeling mixture (Roche) and the T7 (antisense probe ...
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bioRxiv - Microbiology 2023Quote: ... DIG-labelling was obtained using “DIG RNA Labelling Mix” (Roche, Basel, CH) while for following hybridization “DIG Easy Hyb Granules hybridization solution” (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... using DIG labeling (Roche) and purified with the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... DIG-labeled (Roche 11218590910) was used as marker ...
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bioRxiv - Biophysics 2020Quote: ... Anti-Dig antibodies (Roche). Buffer solutions ...
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bioRxiv - Genomics 2020Quote: ... DIG-labeled (Roche 11218590910) was used as marker for all Southern blot experiments.
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bioRxiv - Neuroscience 2020Quote: ... DIG-labelled (Roche 11277073910) RNA probes were transcribed via the T3 promotor from 1ug of purified DNA template and cleaned via ethanol-salt purification with GenElute linear polyacrylamide (Sigma 56575-1ML ...
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bioRxiv - Developmental Biology 2020Quote: ... dig-UTP (Roche, #3359247910), anti-Digoxigenin AP antibody (1:3000 ...
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bioRxiv - Genetics 2022Quote: ... DIG-labeled (Roche 11218590910) was used as the marker.
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bioRxiv - Developmental Biology 2021Quote: ... Positive clones were digested with the appropriate enzyme to linearize the plasmid and anti-sense ribonucleoprobe synthesis was carried out using Sp6 or T7 RNA polymerase (New England Biolabs)+DIG labeled UTP (Roche). Subsequent probes were cleaned up using the RNA Clean Up kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... DIG Wash buffer (Roche) was used to remove excess antibody and amplified C-circles were detected with CDP-Star® kit (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... DIG labeling mix (Roche) and RNase inhibitor (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... DIG-11-dUTP (Roche) and the S2-Fw and S2-Rv primers (Supplemental Table 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti-DIG FAB (Roche) was diluted 1:1000 in blocking buffer and slides were incubated for at least 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Dig-labeled probes were detected with an AP-conjugated anti-Dig antibody (Roche) followed by NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Genetics 2020Quote: ... The DIG probes were detected with anti-DIG-HRP antibody (Roche, Indianapolis, IN) and a coumarin-tyramide color reaction (Perkin-Elmer ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled riboprobes were synthesized following manufacturer’s protocol (DIG Labeling Kit, Roche). Primers used to generate the riboprobes are listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The DIG labeled cRNA probes were synthesized using DIG RNA labeling kit (Roche). The reagents and antibodies were listed in supplemental Table S6.
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bioRxiv - Bioengineering 2024Quote: ... Pre-hybridization was carried out at 48°C for 2 hours in DIG Easy Hyb buffer (Roche). The trnI/A probe was denaturalized for 20 minutes at 68°C and used for hybridization overnight at 48°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Primary antibody incubation was carried out overnight at 4°C using anti-DIG-AP solution (Roche 11093274910) in MAB blocking buffer ...