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Citations for Roche :
1 - 50 of 73 citations for Diethylene Glycol Divinyl Ether stabilized with KOH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... pH 7.6 with KOH) containing 5 mg mL−1 (type A, ROCHE). Oocytes were kept at 19 °C in a Barth’s solution (in mM ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 mM HEPES/KOH) containing a complete protease inhibitor cocktail (Roche, 11697498001) and homogenized ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.6 with KOH) containing 5 mg ml−1 collagenase (type A, ROCHE). Defolliculated oocytes were injected with 50 ng mRNAs of mixed GlyT1 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mM HEPES-KOH pH 7.4) supplemented with cOmplete protease inhibitor cocktail (Roche). Then ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mM HEPES-KOH pH 7.4) supplemented with cOmplete protease inhibitor cocktail (Roche), and cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.25 with 0.5M KOH with addition of 1 U/μl recombinant RNase inhibitor (Roche) immediately prior to experiment and final osmolarity of 315–320 mOsm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5 mM EGTA-KOH pH 8.0, 0.5 mM DTT, 0.5 mM Pefabloc-SC, and 1x Roche Complete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2020Quote: ... resuspended in 25 ml ice-cold lysis buffer (20 mM Hepes-KOH pH 8.0, 0.5 M NaCl, 1 mM EDTA, 0.1% Triton X-100, Roche cOmplete™ EDTA-free protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was then resuspended in HEGX Buffer (20 mM HEPES-KOH, pH 7.2, 0.8M NaCl, 1 mM EDTA, 10% glycerol, 0.2% Triton X-100 and 1 Roche Complete Protease Inhibitor Tablet ...
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bioRxiv - Developmental Biology 2022Quote: ... was bound overnight at 4°C in 25µL per sample of Antibody Buffer (20mM HEPES-KOH pH7.5, 150mM NaCl, 0.5mM spermidine, 0.01% digitonin, 2mM EDTA, 1x Roche cOmplete mini EDTA-free protease inhibitor) ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was removed and the pellet was gently resolved in 200 μl Tween-Wash (0.1% Tween-20, 20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1 Roche Complete tablet-EDTA per 50 ml buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... were added to the nuclei-bound beads for 1hr at room temperature in 25µL of Wash300 Buffer (20mM HEPES-KOH pH7.5, 300mM NaCl, 0.5mM spermidine, 1x Roche cOmplete mini EDTA-free protease inhibitor) ...
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bioRxiv - Developmental Biology 2022Quote: ... was bound in 25µL per sample of Wash150 Buffer (20mM HEPES-KOH pH7.5, 150mM NaCl, 0.5mM spermidine, 1x Roche cOmplete mini EDTA-free protease inhibitor ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Cells were then resuspended in 1 mL of ice cold NE1 Buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5 mM Spermidine, 0.1% TritonX-100, 20% Glycerol, with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Plant Biology 2022Quote: ... Chloroplast were lysed osmotically in buffer 3 (25mM HEPES-KOH, pH 8.0) containing cOmplete protease inhibitor (Roche) and either separated into soluble and pellet fraction by centrifugation for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were incubated with 10 mL of ice-cold buffer A (10 mM Hepes-KOH pH 7.9, 1.5 mM MgCl2,10 mM KCl, 1 mM DTT, 1x Complete Protease inhibitors, Roche) for 1–2 min ...
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bioRxiv - Cell Biology 2024Quote: ... resuspend in 50 µL Dig-300 buffer (20 mM HEPES–KOH, pH 7.5, 300 mM KCl, 0.5 mM Spermidine, 0.01% Digitonin, 1X Roche cOmplete Mini EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... and then resuspended in 1 mL of ice cold NE1 Buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5 mM Spermidine, 0.1% TritonX-100, 20% Glycerol, with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed thrice with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2020Quote: ... were washed three times with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Developmental Biology 2022Quote: ... 500,000 trypsinized ESC were washed a total of three times with Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, Roche complete Protease Inhibitor tablet EDTA free ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were seeded into 10 cm dishes and GFP–TAF2 protein expression induced 18–20 h prior to cell harvest in Wash buffer (20 mM Hepes-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1x EDTA-free Complete Protease Inhibitor, Roche). After incubation with activated BioMag®Plus Concanavalin A magnetic beads (Polysciences ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were lysed in LUMIER lysis buffer (50 mM Hepes-KOH pH 7.9, 150 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 5% glycerol and Roche cOmplete™ Mini protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... samples were checked to ensure that they were bound to the beads and then washed two times with Dig-wash buffer (20mM HEPES–KOH, pH 7.5, 150 mM KCl,0.5 mM Spermidine, 1X Roche cOmplete Mini EDTA-free Protease Inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were washed two times with 300 μL of 1%BSA antibody buffer (20mM HEPES– KOH, pH 7.5, 150 mM KCl,0.5 mM Spermidine, 1X Roche cOmplete Mini EDTA-free Protease Inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... cells from a sub-confluent 15 cm plate were resuspended in isolation buffer (10 mM HEPES-KOH pH 7.4, 1.5 mM MgCl2, 10 mM KCl, EDTA-free complete protease inhibitor cocktail from Roche). After 10 min incubation at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Pooled ventral otocysts were homogenized in lysis buffer (20 mM HEPES KOH, pH 7.4, 150 mM KCl, 10 mM MgCl2, EDTA-free protease inhibitors [Roche, 04693159001] ...
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bioRxiv - Microbiology 2021Quote: ... frozen pellets were thawed on ice in 50 mL conical flasks with pre-chilled lysis buffer (50 mM HEPES – KOH pH 7.6, 500 mM KCl, Roche Protease Inhibitors tablet (0.5 tablet/L of culture) ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 4 hr and washed two times with its resuspending buffer and once with E100 buffer (25 mM HEPES-KOH pH7.5, 10 % glycerol, 100 mM KCl, 2 mM MgCl2, 1 mM EDTA with Roche cOmplete™ ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... diluted with an equal volume of IP buffer (150 mM HEPES-KOH pH 6.8, 150 mM K2OAc, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 1% NP-40, 1x Roche protease inhibitor cocktail and 1 x phosSTOP cocktail ...
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bioRxiv - Cancer Biology 2020Quote: Organoids were incubated in Cell Recovery Solution and nuclear extracts were prepared by resuspending organoids in nuclear extraction buffer A (10 mM HEPES/KOH pH 7.9, 0.1 mM EDTA, 10 mM KCl, 1x Complete® [Roche, 1697498] ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with warm PBS and scraped in homogenisation buffer (10mM HEPES-KOH pH 7.4, 250mM sucrose, 5mM DTT, 1mM EDTA, and protease inhibitor cocktail, Roche). The cells were then homogenized using an Eberbach Corporation (E2355 ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen HEK293E cells were thawed and resuspended in 2x excess of lysis buffer (25 mM HEPES-KOH pH 7.6, 5 mM MgCl2, 50 mM KCl, cOmplete protease inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Genomics 2023Quote: ... 500,000 cells per condition were washed twice in 1 mL Wash Buffer (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Protease inhibitor cocktail; Roche 11836170001). Nuclei were extracted by incubating cells for 10 minutes on ice in 200 μL/sample of cold Nuclei Extraction buffer (NE buffer ...
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bioRxiv - Cell Biology 2023Quote: Cells were resuspended in cold homogenization buffer (250 mM sucrose, 250 mM HEPES/KOH pH 7.4, 1 mM EDTA) supplemented with cOmplete protease inhibitors (Roche) and lysed by Dounce homogenization on ice ...
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bioRxiv - Cell Biology 2023Quote: ... and resuspended in solubilization buffer (50 mM HEPES/KOH pH 7.5, 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche] ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was suspended using Buffer A (25 mM Mops-KOH, pH 7.5, 200 mM NaCl, 10% Glycerol, and cocktail protease inhibitor solution (Roche) with 50-fold dilution) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
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bioRxiv - Neuroscience 2021Quote: DNA was extracted from EDTA-stabilized peripheral blood lymphocytes and subjected to exome capture using NimbleGen SeqCap EZ MedExome (Roche), followed by sequencing on an Illumina NovaSeq platform to a mean coverage of minimum 300x ...
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bioRxiv - Neuroscience 2021Quote: ... and cells were washed twice in 1 ml Wash Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™), followed by addition of 10 µl pre-activated concanavalin A coated magnetic beads (Bangs Laboratories-BP531) ...
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bioRxiv - Developmental Biology 2021Quote: ... 25mM KCl, 5mM MgCl2, 20mM Tricine-KOH pH7.8, 1mM DTT, 0.15 mM spermine, 0.5 mM spermidine, Roche protease inhibitor tablets) supplemented with 1.5ul/ml RNAse Inhibitor (RNasin Plus RNase Inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... then harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES-KOH pH 7.0, 500 mM NaCl, 5 mM β-mercaptoethanol, 1x Protease Inhibitor Cocktail (Roche)) and lysed by sonication ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in fractionation buffer (20 mM HEPES-KOH pH 7.6, 1 mM EDTA, 1x cOmplete protease inhibitor (Roche 11873580001)) ...
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bioRxiv - Genetics 2022Quote: ... The samples were resuspended by 60μL washing buffer (HEPES-KOH, pH = 7.5, 20mM; NaCl, 150mM; Spermidine, 0.5mM and with Roche complete protease inhibitor.). Concanavalin-coated magnetic beads (Polyscience ...
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bioRxiv - Developmental Biology 2024Quote: ... The following day unbound chromatin was removed through seven washes in RIPA buffer (1% NP-40, 0.7% Na-Deoxycholate, 1mM EDTA, 50mM HEPES-KOH, pH 7.55 and 0.5M LiCl with Roche Protease Inhibitor #04693159001) and one in TE buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... nuclei were isolated from 1×105 cells by incubating them in the NE Buffer (20 mM HEPES-KOH, pH 7.9, 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 0.5 mM Spermidine, 1x Roche cOmpleteTM Protease Inhibitor) for 10 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in 4x pellet volume of sucrose buffer (10 mM HEPES/KOH pH 7.5.; 2 mM MgAc; 250 mM Sucrose, 1x Protease inhibitor cocktail [Roche, Germany]) and lysed with ~50 strokes in a tight-fit dounce homogenizer ...