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Citations for Roche :
401 - 450 of 7814 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with a 1 µg/ml solution of the DNA-dye DAPI (Roche) and mounted with Slowfade antifade reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in Buf1*(10 ml Buf.1 + protease inhibitor cocktail (Roche, Basel, Switzerland) and lysed with MULTI BEADS SHOCKER (YASUI KIKAI ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in 1% Triton X-100 buffer with phosphatase and protease inhibitors (Roche). Protein was separated by 4-12% Bis-Tris protein gel (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... Cell Proliferation Reagent WST-1 and Cytotoxicity Detection KitPLUS (LDH) were obtained from Roche (Germany). 5× HOT FIREPol® EvaGreen® qPCR Supermix was acquired from Solis BioDyne (Estonia) ...
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bioRxiv - Bioengineering 2023Quote: ... High Pure RNA Isolation Kit and Cell Proliferation Reagent WST-1 were purchased from Roche. QIAshredder and RNeasy Fibrous Tissue Mini Kit were purchased from Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Roche). To reduce autofluorescence ...
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bioRxiv - Synthetic Biology 2021Quote: GST-tagged CBX8 was purified by re-suspending thawed cell pellets in 30 ml of lysis buffer (1× PBS, 5 mM DTT, 1× EDTA free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN)) per liter of culture ...
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bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended in 1 ml staining buffer containing 0.1 mg/ml RNaseA (Roche Life Sciences) and incubated at room temperature (RT ...
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bioRxiv - Biochemistry 2022Quote: ... Cell membranes were solubilized in the lysis buffer plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were then prepared by incubating minced tissue in 1 mg/ml collagenase (Roche) and 50 U/ml DNase I (Roche ...
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bioRxiv - Genetics 2019Quote: ... Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100, 11836145001, Roche applied science) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche) and analyzed using western blots.
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bioRxiv - Cancer Biology 2023Quote: Du145 (5 × 103) and 22Rv1 (1 × 104) cells were seeded on 96-well E-Plates (Roche). Proliferation was monitored every 1 h and time dependent cell index (CI ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Systems Biology 2019Quote: ... Single cells were collected in 1 μL of cell lysis buffer (1 U RNasein plus [Promega, Madison, WI], 10% RealTime ready Cell Lysis Buffer [Cat# 06366821001, Roche], 0.3% NP40 (Thermo Fisher Scientific), and RNase-free water (TaKaRa ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extract was incubated overnight with anti-HA antibodies (Roche). After over-night incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were incubated with 30 µl Protein G-Agarose (Roche) per ml lysate for 1 hour at 4 °C and washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... followed by incubation with Protein G Agarose beads (Roche, 1124323301) (15μl ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl bed volume of Protein A Agarose (Roche 11134515001) was added the following day to each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitations were performed using Protein G Agarose beads (Roche, 1124323301) or anti-FLAG (M2 ...
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bioRxiv - Genetics 2022Quote: ... 40 μl of protein G agarose beads suspension (Roche 11243233001) (settled beads volume 20 μl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by Lumi-Light Plus detection reagent (Roche).
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bioRxiv - Microbiology 2021Quote: ... 100 µl of protein G-coupled agarose bead resin (Roche) were washed (2 × CL buffer 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 200 ng trypsin (Roche, Basel, Switzerland) shaking at 600 rpm at 37°C for 17 hours ...
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bioRxiv - Cell Biology 2020Quote: GFP-fusion proteins were detected using a monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted using RIPA buffer (Thermo) and proteinase (Roche) plus phosphatase (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Resuspended protein samples were digested with endoproteinases Lys-C (Roche) and trypsin (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested by adding trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and pre-cleared with protein A-agarose beads (Roche, Germany). Primary antibody incubations were carried out for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected with anti-HA antibody (Roche, Cat#11867423001).
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently solubilised in 1% Triton X-100 in PBS containing 1x cOmplete protease inhibitor (Roche) and incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... Infected red blood cells were lysed on ice with chilled hypotonic buffer (1 mM HEPES/NaOH, Roche cOmplete EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with a total of 1 µg of DNA using Fugene 6 (Roche, Branchburg, NJ) according to the manufacturer’s instructions
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were resuspended in 200 µL PBS containing 1× Complete EDTA-free protease inhibitor cocktail (Roche) and sonicated by Bioruptor II (BMBio) ...
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bioRxiv - Immunology 2020Quote: Skin samples from mice were enzymatically digested to release cells using 1 mg/ml collagenase D (Roche), 0.5 mg/ml dispase (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell viability was measured by trypan blue exclusion or WST-1 assays (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated with 20 mU ml- 1 Vibrio cholerae-derived neuraminidase (sialidase) (#11080725001, Roche, Switzerland) for 1 h at 37°C on a cell shaker ...
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bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...