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Citations for Roche :
201 - 250 of 7821 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with DAPI (1/2000 in sterile PBS from 1 mg/ml stock, Roche). Sorted cells were re-suspended in medium to reach a 2.5 million cells/ml concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Neuroscience 2021Quote: Protein samples were extracted from cells using ice-cold RIPA buffer with complete protease inhibitor mixture (Roche Applied Science, 04693124001). For immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were isolated from cell cultures in mid-exponential phase in Hepes 50mM supplemented with EDTA-free protein inhibitor cocktail (Roche). Cell walls were broken by three 30-s cycles at 10000 rpm in the presence of micro glass beads (200-400 µm diameter Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated with Protein A/G-Agarose beads (Cat# 11 134 515 001/ 11 243 233 001, Roche), and either ARP3 or normal Rabbit IgG antibody for 2 h at 4°C with rotating ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysate proteins were extracted using RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Cell Biology 2024Quote: ... Protein purification started by quickly thawing the cells in ∼15 mL room-temperature 10mM imidazole with protease inhibitor cocktail (Roche) and freshly added 200µM PMSF ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-GFP (Roche, Cat # 11814460001) or α-BAK1 antibodies 55.
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cell proliferation was quantified after 48 h of incubation using cell proliferation reagent WST-1 (Roche, 11644807001) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was treated with 1 ml of Red Blood Cell Lysis Buffer (Roche, Solna, Sweden) for five minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Genetics 2020Quote: ... cells were lysed in 1x RIPA buffer (Cell Signalling) with 1% PMSF and protease inhibitor cocktail (Roche). For gel-shift assays54 ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Molecular Biology 2019Quote: Total proteins were extracted in RIPA buffer containing 1 mM sodium orthovanadate and Complete Protease Inhibitor Cocktail (Roche Applied Science) and quantified using the Pierce™ BCA protein assay kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were diluted to a final total soluble protein concentration of 1 mg/ml in TBS containing cOmplete mini protease inhibitor tablet (Roche). For AD ...
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-Green Fluorescent Protein overnight at 4°C (Clones 7.1 and 13.1; 1:50 dilution, Roche, Mannheim, Germany) and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein extracted using RIPA buffer with protease inhibitor cocktail mix (1 Complete MINI EDTA-free protease inhibitor tablet (Roche), 25 μg/mL calpain inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
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bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was assessed using colorimetric WST-1 reagent (Roche, 11644807001) as per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: Cells were stained with antibodies against GFP (Roche; 1181446001; 1:1000), GFP (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... After addition of 10 μl WST-1 cell Proliferation Reagent (Roche) cells were incubated for 1 h at 37°C before absorbance was measured at 450 nm with a wavelength correction set at 620 nm ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mL Red Blood Cell Lysis buffer (Roche cat. No 11814389001) was then added to the tube and the cell mixture was placed on a rocker for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... as recommended by the manufacturer (Cell Proliferation Kit 1, 11465007001 Roche). After overnight incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were blocked overnight (1% Roche Western Blotting Reagent (WBR), Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6,000 cells per well and using WST-1 reagent (Roche). Absorbances were measured at 450nm and 650nm on a Versamax turntable microplate reader (Molecular Devices) ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...