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Citations for Roche :
201 - 250 of 1762 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... X-tremeGENE 9 DNA Transfection reagent (Roche, #06365787001) was diluted in Opti-MEM serum-free media ...
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bioRxiv - Genomics 2021Quote: ... and 0.02U/μl KAPA HiFi DNA polymerase (Roche). After dispensing plate was quickly spun down before incubated in PCR as follows ...
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bioRxiv - Genomics 2021Quote: ... and 0.02U/μL KAPA HiFi DNA polymerase (Roche)) ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... followed by ligation using T4 DNA ligase (Roche) and annealed and phosphorylated complementary oligos (Table S2) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform/isoamylalcohol (Roche), precipitated using isopropanol and resuspended in TE ...
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bioRxiv - Plant Biology 2022Quote: ... and DNA was removed by DNase I (Roche) treatment ...
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bioRxiv - Biochemistry 2023Quote: ... using X-tremeGene HP DNA Transfection Reagent (Roche), with 10 μg of pcDNA-GST ...
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bioRxiv - Cell Biology 2022Quote: ... or X-tremeGene HP DNA Transfection Reagent (Roche), or HiPerFect Transfection Reagent (QIAGEN).
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bioRxiv - Microbiology 2023Quote: ... genomic DNA extracted (KAPA Express Extract, Kapa BioSystems) from wild or transfected parasites were analyzed by PCR using standard procedures with the primers P1-P3 ...
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bioRxiv - Microbiology 2023Quote: ... water and FastStart Essential DNA Probes Master (Roche). The sequences of primers and hydrolysis probes ...
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bioRxiv - Neuroscience 2023Quote: ... using the FastStar Essential DNA Green Master (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... using X-tremeGENE 9 DNA transfection reagent (Roche) according to the protocol supplied by the vendor ...
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bioRxiv - Genomics 2022Quote: ... Cell-Free DNA Collection Tube (Roche; Roche, 07785666001), and LBgard Blood Tube (Biomatrica ...
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bioRxiv - Developmental Biology 2023Quote: ... Xtremegene HP DNA transfection reagent (Roche Applied Science) was utilized per manufacturer’s directions to co-transfect the cells with 4 µg pBABE-neo-SV40 large T ...
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bioRxiv - Microbiology 2023Quote: ... After DNA ring ligation using T4 ligase (Roche) at 16°C overnight ...
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bioRxiv - Genomics 2022Quote: ... Cell-Free DNA Collection Tube (Roche; Roche, 07785666001), and LBgard Blood Tube (Biomatrica ...
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bioRxiv - Microbiology 2022Quote: ... and HyperPlus DNA kits (Roche Diagnostics, Indianapolis, IN). Nextera libraries were generated according to the manufacturer’s protocol with the lone modification being an extension of the tagmentation reaction time to 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5U of KAPA HiFi HotStart DNA polymerase (Roche), 12.5 μL of 5× KAPA reagent ...
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bioRxiv - Microbiology 2023Quote: ... using the DNA KAPA HyperPrep library kit (Roche). Paired-end 150 bp sequencing was generated using the NovaSeq S4 platform (Illumina ...
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bioRxiv - Genomics 2024Quote: ... and 2U KAPA HiFi HotStart DNA Polymerase (Roche)) was then added to each well using a MANTIS Liquid Dispenser (Formulatrix) ...
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bioRxiv - Bioengineering 2023Quote: ... using X-tremeGENE 9 DNA Transfection Reagent (Roche). Lentiviruses were packaged using 293FT cells by co-transfection of lentiviral transfer plasmids with pMD2.G (Addgene #12259 ...
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bioRxiv - Bioengineering 2023Quote: ... using X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Cell Biology 2024Quote: ... X-tremeGENE™ HP DNA Transfection Reagent (Roche) was used according to the manufacturers protocol ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted and purified with the MagNA Pure LC instrument and the MagNA Pure DNA isolation kit III (Roche Diagnostics), according to the recommendations of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire ChIP DNA or 10 ng DNA in the case of ChIP input was used to make DNA library with the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... The normalization of DNA amount was performed by quantifying albumin gene copies with qPCR using human genomic DNA (20 ng/µl) standard (Roche, #11691112001) as we previously described16.
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bioRxiv - Plant Biology 2019Quote: ... An Illumina DNAseq library was constructed for polishing the PacBio based assembly using 1ug of DNA the same high molecular weight DNA prep with the KAPA HyperPrep Kit (Kapa Biosystems). The Illumina DNAseq library was sequenced on an Illumina HiSeq4000 under paired end mode (150 bp ...
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bioRxiv - Biochemistry 2022Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: LP-WGS libraries were prepared with 50 ng of sonicated tumor DNA (extracted from FFPE as described above) and patient-matched buffy coat DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Illumina single index Tru-Seq adaptors (idtdna.com ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were prepared using 100-500 ng of cell free DNA (cfDNA) or sonicated genomic DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: Whole genome sequencing libraries were generated from 1000 ng of genomic DNA spiked with 0.1% (w/w) unmethylated λ DNA (Roche Diagnostics) and fragmented to 300–400 bp peak sizes using the Covaris focused-ultrasonicator E210 ...
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bioRxiv - Biochemistry 2021Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: Random primed DNA probes were generated by using the Random Primed DNA Labeling Kit from Roche (cat# 11-004-760-001). Generally ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified using 22 cycles of PCR amplification from approximately 300 µg genomic DNA (10 µg DNA/100 µL reaction) using 2X KAPA HiFi HotStart ReadyMix (Roche KK2602), a combination of 8 staggered P5 primers ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was also used to quantify gene dose of tTa and MAPT in genomic DNA using DNA Green reagents (Roche 06402712001). Primer sequences for tTa are forward ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were performed using Phusion® Hot Start High-Fidelity DNA Polymerase (Finnzymes) or Expand High Fidelity DNA polymerase (Roche). Chromosomal DNA from PAO1 or KT2440 was normally used as DNA template in PCR reactions except for the amplification of the P ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection with full-length nV5-WT or nV5-P112L (0.8 μg DNA/well) was performed 24 h post-seeding using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Basel, Swityerland). 24 h post-transfection ...
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bioRxiv - Genomics 2022Quote: ... The labeled DNA fragment was ethanol-precipitated with sonicated salmon sperm DNA (Cat# D7656, Merk) and Escherichia coli tRNA (Cat# 10109541001, Roche Applied Science), and mouse Cot-1 DNA (Cat# 18440016 ...
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bioRxiv - Microbiology 2020Quote: Quantification of precipitated DNA was determined using real-time quantitative PCR (qPCR) with SYBER Green in FastStart Essential DNA Green Master (Roche, Basel, Switzerland). Each resultant DNA was diluted in nuclease-free water and was analyzed in triplicated for EBV-associated genes and CTCF binding sites ...
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bioRxiv - Cancer Biology 2019Quote: ... Clones were screened for knockout of target genes by Western Blot and/or by allelic sequencing with custom primers (Integrated DNA Technologies; Table S2) after processing genomic DNA with the KAPA Mouse Genotyping Kit (KAPA Biosystems KK7352) and TOPO TA Cloning Kit (Thermo Fisher Scientific K457501).
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bioRxiv - Genetics 2020Quote: ... A small portion of the vitelline glands from the posterior part of each fluke was used for DNA extraction using the High Pure DNA Extraction Kit (Roche, Mannheim, Germany) following the manufacturer’s protocols ...
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bioRxiv - Genetics 2019Quote: ... after which genomic DNA was prepared from the tissue using the KAPA Express Extract DNA Extraction Kit (Kapa Biosystems, cat. nr. KK7103). For both Gulo and Slc2a10 the DNA was PCR-amplified using specific primers detecting the wild type and mutant alleles ...
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bioRxiv - Plant Biology 2023Quote: ... Converted DNA was amplified for 23 cycles using U1 and U2 primers and KAPA HiFi HotStart Uracil+ DNA polymerase (Roche, Pleasanton, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 0.2 μl of FastStart Taq DNA polymerase (Roche, 04659163103) and water ...
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bioRxiv - Genomics 2021Quote: ... The purified DNA was quality-controlled using qPCR (Roche) and Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... using FastStart Essential DNA Probes Master (Roche, Basel, Switzerland) and the following RealTime ready Single Assays (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNAs were removed using DNase I (Roche 04716728001) and RNAs were cleaned using RNA Clean & Concentrator (Zymoresearch R1013) ...
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bioRxiv - Genomics 2020Quote: ... 0.4μl KAPA HiFi DNA Polymerase (Roche, cat no. KK2102), 0.2μl 10% Tween-20 and 1.6μl custom barcoded primers (3.75uM each ...
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bioRxiv - Genomics 2020Quote: ... DNA concentration was quantified using the KAPAQuant system (Roche) on the QuantStudio 6 Flex (Life Technologies) ...