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Citations for Roche :
101 - 150 of 1569 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... for 90 min at 37 °C and digested overnight at 37 °C in 1.5 mg/ml collagenase B solution (Roche). Then ...
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bioRxiv - Bioengineering 2020Quote: Muscles were dissected and minced to a pulp using forceps then digested with collagenase B solution (0.75 U/mL final, from Roche) with dispase II (1.2 U/mL final ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... The sinoatrial node explants from old animals were first incubated for 20 min in Low-Calcium Tyrode’s containing twice the concentration of collagenase B used in the young (Cf = 1.08 U/ml, Roche 11088807001), followed by the same 30 min digestion with the three other enzymes at the same concentration used for young tissues ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Cell Biology 2021Quote: ... for 90 minutes at 37°C and digested overnight at 37°C in 1.5 mg/ml collagenase B solution (Roche) under continuous agitation ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed (75mM NaCl, 50mM HEPES pH 8.5, 10mM Sodium pyrophosphate, 10mM Sodium Fluoride, 10mM B-Glycerophosphate, 10mM Sodium Orthovanadate, Roche complete mini EDTA free protease inhibitors ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Serum lipid panel was measured using Cobas b 101 POC system and its lipid disc (both from Roche Diagnostics). For liver cholesterol measurements ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Cell Biology 2019Quote: Zebrafish samples for Western blot analysis were deyolked and directly lysed in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 10 ul per embryo unless otherwise indicated ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC cells for Western blot analysis were lysed directly in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 500ul per T-25 culture flask.
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Genomics 2019Quote: ... The crosslinked chromatin was lysed in 130 uL of Buffer B (LowCell# ChIP kit, diagenode) supplemented with Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... the linear segment relating the fish’s CoM to the center of the stimulus and b) the linear segment relating the fish’s CoM to its snout (Jornod and Roche 2015). These measures (4 and 5 ...
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bioRxiv - Cell Biology 2022Quote: All strains expressing NUP-RITE constructs were grown to mid log phase in SCD supplemented with 300 μg/mL hygromycin B (Roche) to select for non-recombined cells ...
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bioRxiv - Systems Biology 2019Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM b-glycerophosphate, 5 mM NaF, 1 mM Na3VO4 and protease inhibitors (Roche cOmplete ULTRA Tablets ...
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bioRxiv - Immunology 2019Quote: ... memory B cells were plated at 6 B cells in 55 μl per well into 96 × 384-well plates in B cell media supplemented with 100 U ml−1 IL-2 (Roche), 50 ng ml−1 IL-21 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SPD-5 (1:1000, a generous gift from B. Bowerman, Hamill et al., 2002) and anti-GFP (1:500, Roche) overnight at 4°C and with secondary antibodies Alexa488 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac tissue sections were either stained for 5-bromo-4-chloro-3-indolyl-b-galactosidase (Xgal; Roche Cat #XGAL-RO) or immunostained with rabbit anti-mouse polyclonal Ror2 (provided by Dr ...
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bioRxiv - Immunology 2020Quote: ... Cyclophilin B (control) and PPARγ siRNA (SMARTPool, Dharmarcon) were diluted in DOTAP (1,2-dioleoyl-3-trimethylammonium-propane; Roche Applied Sciences). The mix was gently mixed and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-Time qRT-PCR was performed using the Real-Time 2xHS-PCR Master Mix Sybr B (A&A Biotechnology) on a LightCycler 480 II qPCR System (Roche) with custom-designed primers for Gli1 (Fwd ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 1 mL Equilibration Buffer B containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche) and 0.2 mg/mL lysozyme ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended and homogenized in buffer B supplemented with 2 mM MgCl2 and 1x protease inhibitor (Roche). The protein was extracted by incubating with 1% GDN (Anatrace ...
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bioRxiv - Genomics 2023Quote: ... 1ng of Cut and Run DNA was used to make libraries using the KAPA Hyper prep kit and SeqCap adapters A &B (Roche) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... dTRPA1-A or dTRPA1-B in pMT vectors were co-transfected with pBS-puro using X-tremeGENE 9 DNA transfection reagent (Roche), and stable cells were selected and maintained in the presence of 10 µg/mL puromycin ...
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bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies were diluted in antibody dilution buffer (catalog# 5280524001, Roche Diagnostics ...
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bioRxiv - Developmental Biology 2019Quote: ... POD antibodies (Roche) in western blocking reagent overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... 300 ng of total RNA from three biological replicates per B cell subset was used as input for the KAPA stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems). Libraries were assessed for quality on a bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... HindIII treatment was performed at 37°C for 1 h in 1x reaction buffer B with 10U of HindIII enzyme per reaction (HindIII, 10656321001, Roche, Switzerland). Mung bean nuclease treatment was performed at 30°C for 30 min in 1x mung bean nuclease reaction buffer with 1U per reaction (Mung Bean Nuclease ...
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bioRxiv - Biophysics 2022Quote: ... embryos were washed (4 X 10 min) in PBTx and blocked in PBT-B (1× PBS, 20% (v/v) western blocking reagent (Roche, 11921673001), 2 mM ribonucleoside vanadyl complex (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... using the Elecsys® Intact hCG+b electrochemiluminescence immunoassay (ECLIA) and the fully automated modular analytics E170 testing system from Roche Diagnostics (Mannheim ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Biochemistry 2023Quote: ... Selection for dominant markers was performed on YPD-based medium supplemented with 200 μg/mL G418 (Beyotime, ST081), 250 μg/mL clonNAT (KLBIOS, PD20181786) or 300 μg/mL hygromycin B (Roche, 10843555001).
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Molecular Biology 2021Quote: ... Putative hygromycin and neomycin-resistant candidates transformants were screened on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Screening of hygromycin- and neomycin-resistant transformants was performed on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1, LS005477] and collagenase type B [Roche, 0.35 U·ml-1, 11088807001]). The left ventricle was collected after 10 minutes’ digestion and cut into small pieces ...
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bioRxiv - Neuroscience 2019Quote: ... Tongues were removed and injected beneath the lingual epithelium with 0.2 mL enzyme solution (3 mg Dispase II, 0.7 mg collagenase B (Roche diagnostics, Indianapolis, IN, USA) and 1 mg Trypsin Inhibitor in 1mL Tyrode’s) ...
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bioRxiv - Cell Biology 2020Quote: ... Telomeric repets were calculated as described (Gil and Coetzer, 2004a, b; Vasilishina et al., 2019) using a LightCycler 2,0 (Roche Diagnostics, Basilea, SWI). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody solution containing anti-DIG-POD antibody (1:250, Roche, RRID:AB_514500) and mouse anti-GFP antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: Primary antibody dilutions were used as follows: rat α-HA antibody (Roche) 1:2,000 ...