Labshake search
Citations for Roche :
151 - 200 of 231 citations for Chikungunya virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... 4°C) the lysate was transferred to 50 μl HA bead slurry (anti-HA affinity matrix, Roche) or 30 μl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
-
bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
-
bioRxiv - Biochemistry 2022Quote: ... and 10% glycerol] containing the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche). The lysate was frozen in liquid nitrogen and stored at −80 °C.
-
bioRxiv - Cell Biology 2022Quote: ... cell lysate was incubated with agarose beads conjugated with either mouse IgG or mouse anti-GFP antibodies (Roche) for 3h at 4°c under gentle agitation ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from cell lysates which were previously lysed by external lysis buffer (Roche, Penzberg, Germany) as described above ...
-
bioRxiv - Cancer Biology 2020Quote: ... lysates were prepared in RIPA lysis buffer supplemented with complete ULTRA protease inhibitor cocktail tablets (Roche, Basel, Switzerland) and western blotting was performed as demonstrated before (Nair et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... whole cell lysates were obtained by lysing the cells in RIPA buffer containing cOmplete Protease Inhibitor Cocktail (Roche). Following sonication ...
-
bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The nuclear lysates were diluted 2 fold with IP buffer (20mM HEPES, pH 7.9, 0.2M NaCl, complete protease inhibitors (Roche) and then flag-beads (Anti-Flag Magnetic Beads ...
-
bioRxiv - Cell Biology 2023Quote: Whole cell lysates were prepared via scraping cells in PBS containing protease inhibitors (Complete Protease inhibitor cocktail, Roche). Cells were pelleted and resuspended in hot SDS lysis buffer (0.5 % SDS ...
-
bioRxiv - Synthetic Biology 2023Quote: ... We incubated 5μl of clarified crude lysates containing rAAV particles with 50 units (U) of DNAse I (Roche) for 15hr at 37°C ...
-
Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... whole cell lysates were prepared using 2x Laemmli buffer containing phosSTOP and protease inhibitor cocktail (Roche, Mississauga, ON). Lysates were passed through a 27-gauge needle ten times ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
-
bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
-
bioRxiv - Cell Biology 2019Quote: ... Beads were removed by centrifugation (twice at 1,000 xg) and then pre-cleared lysates were supplemented with anti-HA high affinity monoclonal antibody (clone 3F10, Roche) at 2 µg/ml final concentration along with 20 µl Protein G Sepharose beads and incubated at 4° C for 4 hours on a rotating wheel ...
-
bioRxiv - Molecular Biology 2019Quote: Parasite were isolated from rings and early trophozoite stages and lysates were prepared in 1X PBS containing 0.5% TritonX-100 and protease inhibitors (Roche) by sonication (amplitude 30 ...
-
bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
-
bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
-
bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
-
bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... or His6 (full length mouse MLKL and pseudokinase domains) tags and captured from lysates using glutathione resin (UBP Bio) or HisTag Ni-NTA resin (Roche) respectively ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μl of the post-nuclear supernatant was saved as the input lysate and 450 μL was incubated with 5μg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 ml of the lysates prepared with IP extracation buffer from 0.5 g of leaves was incubated with anti-HA Affinity Matrix (Roche 11815016) for o/n in a rotary shaker at 4 °C ...
-
bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared in 2× Laemmli loading buffer supplemented with cOmplete protease inhibitor and PhosSTOP phosphatase inhibitor tablets (Roche), 1 mM PMSF ...
-
bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
-
bioRxiv - Microbiology 2020Quote: Whole cell lysates were obtained by lysing cells in RIPA buffer supplemented with a cOmplete Mini protease inhibitor cocktail (Roche). Protein concentrations were measured using a BCA assay (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: Proteins from brain tissue lysates or primary cortical neurons were extracted using RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Total lysates were obtained by 30 sec ...
-
bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2021Quote: Lysates were prepared from tumor tissue or cultured cells using RIPA buffer supplemented with fresh protease and phosphatase inhibitors (Roche). Protein quantification was performed using the BCA Protein Assay (G Biosciences ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 5% glycerol with the addition of 1 EDTA-free cOmplete protease inhibitor cocktail tablet per 50 mL lysate (Roche). Cells were lysed by sonication on ice with stirring and the lysates were clarified by centrifugation at 20,000 g for 30 minutes at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates were prepared following euthanasia of zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Complete ULTRA Tablets, Roche), followed by homogenisation via probe sonication ...
-
bioRxiv - Cell Biology 2019Quote: Protein lysates from whole pancreas were prepared in 1 mL of RIPA buffer supplemented with phosSTOP phosphatase and protease inhibitors (Roche). Protein concentrations were measured using the BCA kit (Thermo Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
-
bioRxiv - Cell Biology 2022Quote: Protein lysates were collected from worms using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet ...
-
bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated with Protein A/G-Agarose beads (Cat# 11 134 515 001/ 11 243 233 001, Roche), and either ARP3 or normal Rabbit IgG antibody for 2 h at 4°C with rotating ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged at 10,000 x g for 15 minutes to pellet and remove insoluble material after which the remaining soluble lysate was incubated with anti-HA sepharose beads (Roche) for 4 hours at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... 4°C), the lysate (IN, input) was transferred to either 50 µl of HA bead slurry (anti-HA affinity matrix, Roche) or 50 µl c-myc bead slurry (EZview red anti-c-myc affinity gel ...