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Citations for Roche :
101 - 150 of 1673 citations for Caspase 9 CASP9 Antibody Pair since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfections were carried out using X-tremeGENE 9 DNA transfection reagent (Roche Diagnostics) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Vectors were transfected into HEK293FT cells using X-tremeGENE 9 DNA Transfection Reagent (Roche). Retroviral supernatants isolated at 48 h were diluted 1:1 in culture medium and used to infect the ARID1A-wildtype ES2 and KLE cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... Ki-67 (rabbit monoclonal, clone name 30-9, Ventana Roche, 790-4286, 1:400), cleaved caspase 3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Transient transfections with B16-F1 cells were carried out with X-tremeGene 9 (Roche) and replaced with normal growth media 4-6 hours after transfection ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The ligation products were amplified with 9 PCR cycles using KAPA Hifi kit (Roche, P5 universal primer and P7 indexed primer D7XX) ...
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bioRxiv - Genomics 2019Quote: ... we transiently transfected 293T cells using ExtremeGENE 9 according to the manufacturer’s protocol (Roche). After 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... and precB5R-N+GPC by using X-tremeGENE 9 (Roche Diagnostics K.K., Tokyo, Japan). Cells transfected with each plasmid were then infected with m8 at a multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were transfected into HEK293T cells using X-tremeGENE 9 (Roche # XTG9-RO) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The residual 9 μL cDNA were subsequently amplified using Kapa HiFi HotStart Readymix (Roche) at a 1x concentration together with 250 nM UP-primer (Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were immunostained for Ki67 (clone 30-9 at 1:7, Ventana- Roche), CD8 (clone SP57 at 1:7 ...
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bioRxiv - Cell Biology 2023Quote: ... jetPEI-DNA transfection reagent (Polyplus-transfection) and X-tremeGENETM 9 DNA Transfection Reagent (Roche) were used to perform plasmid transient transfection according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... containing 5 µl of X-tremeGENE 9 transfection reagent (XTG9-RO Roche, Sigma-Aldrich). Three days after transfection ...
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bioRxiv - Microbiology 2024Quote: ... pH 9 – adjusted with HCl) and then incubated with CDP-Star substrate (Roche, Germany) for 5 min in darkness ...
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bioRxiv - Genomics 2021Quote: ... a junction PCR was performed using an intron-spanning primer pair (Junction_fwd and Junction_rev) and 8 μl cDNA with the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 15 cycles ...
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bioRxiv - Genomics 2019Quote: ... Double sided size selection was performed using KAPA Pure Beads to retain only fragments within the 250-450 base pair range using the manufacturer’s protocol and eluted in 40 μl of TE buffer (Roche Sequencing Solutions ...
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bioRxiv - Genetics 2020Quote: ... Customized oligonucleotide probes were designed to capture the exons and 30 base pairs of adjacent sequences using NimbleGen (Roche) online oligonucleotide probe design system.
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids were transfected in HeLa and YFP-Parkin HeLa using Xtreme Gene 9 (Roche) according to manufacturer’s directions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.3mg or 1mg/ml/kg midazolam (Hypnovel, Roche, diluted with 0/9% w/v saline) fifteen minutes prior to a standard punishment session ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with siRNA oligonucleotides using X-treme GENE 9 DNA Transfection reagents (Roche) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 150-300 ng of indicated construct using XtremeGene 9 transfection reagent (Roche) according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2021Quote: Transfection of CRISPR/Cas9 constructs was performed using X-tremeGENE 9 DNA Transfection Reagent (Roche), with 4.0 μg plasmid per 200.000 cells/well in a six-well plates ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 µg sgRNA plasmid using X-tremeGENE™ 9 DNA transfection reagent (Roche 06365809001). One day after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using the X-treme GENE 9 DNA transfection reagent (Roche Applied Science) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Nup96-GFP KI U2OS cells were transfected using X-tremeGENETM 9 DNA Transfection Reagent (Roche), and 24h after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were deparaffinized followed by antigen retrieval in CC1 buffer (pH 9, 95°C; Roche), endogenous peroxidase blocking ...
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Sublibrary oligo pools were amplified individually using window-specific primer pairs and KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, IN, USA) with a final template concentration of 1:2000 resuspended oligo array and annealing temperatures of either 60 °C or 65 °C (depending on performance of individual primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Roche). The libraries were built from 3 to 5 different mice per genotype ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Primer pair mix with 7 µL of 2X SYBR green (LightCycler 480 SYBR Green I Master; Roche; Cat No. 04707516001) and 1 µL of 10 µM of forward and reverse primer were prepared for all the gene targets ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 9 µl of qPCR reaction mix containing 5 ul LightCycler 480 Probes Master (Roche, Cat#04707494001), 3.4 ul of Nuclease Free H2O (Ambion ...
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bioRxiv - Cell Biology 2022Quote: ... The construct was then sequenced and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and GFP positive single cells were sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Molecular Biology 2022Quote: ... structure in the 5’UTR and 70 ng of firefly luciferase (FLuc)-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... or a HP50 hairpin structure in the 5’UTR and 2 ng of FLuc-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Bioengineering 2023Quote: ... Transfections of HEK293T and 293FT cells were performed using X-tremeGENE 9 DNA Transfection Reagent (Roche). Murine Stem Cell Viruses (MSCV ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Neuroscience 2021Quote: ... The sequences of the specific forward and reverse primer pairs were constructed using the Primer-BLAST tool or using the “Universal Probe Library” software (Roche Diagnostics). Sequences of the different primer pairs used are listed in Supplementary Table S1 for humans and Supplementary Table S2 for rats ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Microbiology 2019Quote: ... The resulting assemblies were subsequently fragmented in silico into successive sequences of 2000 base pairs overlapping by 1900 bp and were then merged using 454 Life Sciences’s software Newbler (Roche, Basel, Switzerland) as previously described (Hugerth et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina compatible adapters (IDT, Inc) were ligated to the mate pair fragments and amplified with 8-12 cycles of PCR (KAPA Biosystems).
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bioRxiv - Developmental Biology 2021Quote: ... was carried out using 10 ng of cDNA and 100 nM of each primer pair with a Roche LightCycler 480 machine and LightCycler 480 SYBR Green I Master 2X (Roche, 04887352001). The PCR program was ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... 1 µl of RCA product was used as a template with specific primer pairs and Kapa Hifi Hotstart Ready Mix (Kapa Biosystems, USA) using the following thermal cycling protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction qPCRs were set up by dispensing crude gDNA and premixed junction primer pairs into a LightCycler® 1536 Multiwell Plate (Roche 05358639001) containing 1x LightCycler® 1536 DNA Green mix (Roche 05573092001) ...
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bioRxiv - Genomics 2020Quote: ... DNA was amplified by 9 cycles of PCR using the Kapa Hifi Uracil + DNA polymerase (KAPA Biosystems) according to the manufacturer’s protocol ...