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Citations for Roche :
501 - 550 of 1222 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Each resulting denatured protein sample was chemically reduced with 100 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated using 100 mM iodoacetamide for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated overnight at 4°C with a 1:2000 dilution of anti-digoxigenin antibody (Roche) in blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml) (Roche). Damaged livers (48h-72h after APAP injection ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were allowed to dry overnight at 37°C prior to staining with SYBR green (Roche, S7563). Images were acquired on a DeltaVision integrated microscope system using the Applied Precision SoftWoRx acquisition software mounted on an IX71 Olympus microscope with a 10x air objective (Imsol) ...
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bioRxiv - Physiology 2022Quote: ... After mincing muscles were enzymatically dissociated at 37°C for 45 min in 0.2% collagenase A (Roche) and 1 mg/ml dispase (GibcoBRL) ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were probed overnight at 4 °C with anti-GFP primary antibodies (Roche 11814460001; 1:1000) and then with anti-mouse peroxidase-conjugated secondary antibodies (Jackson Immunoresearch # 115-035-146 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... washed in KPBS and incubated for 15 minutes at 37 °C in presence of proteinase K (Roche) at 0.01 mg⁄mL in an appropriate buffer (0.1M Tris–HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat. Nr. 11418025001) at 37 °C for 13 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by resuspension in 1 volume of Buffer C (5 mM Hepes pH 7.9, 26% glycerol, 1.5 mM MgCl2, 0.2 mM EDTA, 1xPIC (Roche) and 0.5 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... Then samples were incubated overnight at 4°C with the following antibodies: anti-HA clone 3F10 (Roche) or anti-M57 clone M57.02 (Center for Proteomics ...
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bioRxiv - Physiology 2020Quote: ... The plasma was separated and stored at −80°C for insulin assay according to manufacturer’s instructions (Roche).
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bioRxiv - Microbiology 2019Quote: ... Purified parasites treated at 12°C for 1 h with freshly prepared 1 mg/mL pronase (Roche)/0.01% saponin/PBS before washing ...
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bioRxiv - Neuroscience 2019Quote: ... Ganglia were digested at 19°C in L15 media containing 10 mg/ml Dispase II (Roche Diagnostics) for 18-19 h or for experiments from animals from Alacrity at 35°C for 2 h ...
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bioRxiv - Biophysics 2019Quote: Analyses of NADP(H) cleavage were carried out at 22 °C using 1000 μM NADP(H) (Roche) and 0.5 μM Nocturnin ...
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bioRxiv - Developmental Biology 2021Quote: ... They were incubated overnight at 4°C with 1:4000 alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche) in buffer 2 ...
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bioRxiv - Bioengineering 2021Quote: ... then digested at 37°C for 45 min in IMDM with 3.5 mg/mL collagenase D (Roche) before being passed through a 70μm filter ...
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bioRxiv - Microbiology 2019Quote: ... Crosslinking was reversed overnight at 65 °C in the presence of 300 μg of Proteinase K (Roche). DNA was purified using phenol/choloroform/isoamyl alcohol extraction followed by EtOH precipitation ...
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bioRxiv - Immunology 2020Quote: Harvested lymph nodes were subjected to digestion at 37°C in DNase I (100 mg/mL, Roche), Collagenase P (200 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... FL fragments were treated for 20 minutes at 37°C with 100 μg/mL of Liberase (Roche) to obtain a cell suspension ...
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bioRxiv - Neuroscience 2022Quote: ... Following an overnight incubation at 4 °C with rat anti-HA (1:500, monoclonal, Roche, Cat # 11867423001) or mouse anti-GAPDH (1:25.000 ...
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bioRxiv - Immunology 2022Quote: ... was incubated for 30m at 4 °C and detection was achieved using UltraView DAB detection kit (Roche). Protein expression was scored by a board-certified pulmonary pathologist blinded to cohort status or treatment group ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% SDS) at 50°C for 15 min and finally washed with 20 ml washing buffer (Roche) at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The IVT reactions were incubated at 37 °C for 4 h and digested with DNase I (Roche). RNA was purified by denaturing PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were treated at 37 °C for 2 h with 500 µL of RNAse A (Roche) 2 mg/mL and after 30 min with 200 µL of pepsin (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The samples were then incubated overnight at 4°C with anti-fluorescein-POD (1:2000, Roche, #11426346910).
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bioRxiv - Immunology 2023Quote: ... Washed bladders were incubated for 1 h at 37 °C in 0.34 U/mL of Liberase (Roche) in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) the lysate was transferred to 50 μl HA bead slurry (anti-HA affinity matrix, Roche) or 30 μl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Bioengineering 2024Quote: ... Pre-hybridization was carried out at 48°C for 2 hours in DIG Easy Hyb buffer (Roche). The trnI/A probe was denaturalized for 20 minutes at 68°C and used for hybridization overnight at 48°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... GRA16HA (and other HA-tagged proteins) was detected using rat anti-HA antibodies (Roche) while GRA24MYC was detected using rabbit anti-MYC tag antibody 9E10 (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2019Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected with a mouse anti-GFP antibody (1:5000; Roche). Immunoblot results were quantified using Image J software (v1.8.0).
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were then extracted in an IP buffer with a phosphatase inhibitor (Roche, PhosSTOP), centrifuged at 1700g for 5 min and filtered through miracloth ...
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bioRxiv - Cancer Biology 2019Quote: Protein was extracted from cultured cells in RIPA buffer containing a protease inhibitor (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: Total protein was collected in radioimmunoprecipitation buffer (RIPA) with PhosSTOP (Roche Diagnostics, Mannheim, Germany) and Complete Mini protease inhibitor cocktail tablets (EMD Chemicals Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... Following a 30 min preclear using blocked Protein A or G agarose beads (Roche), the chromatin was incubated with 5 µg antibody overnight at 4 °C (antibodies are listed in Table S6) ...
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bioRxiv - Cell Biology 2019Quote: ... Treated cells were lysed in protein lysis buffer with protease and phosphatase inhibitors (Roche). Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems ...