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Citations for Roche :
101 - 150 of 225 citations for Biotin Rabbit Polyclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Bound biotin peptides were then detected by incubating with 1 U/ml of HRP-conjugated streptavidin (Roche) on ice for 45 min in PBS/0.1% Triton X-100 ...
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bioRxiv - Genomics 2021Quote: ... rp49 5’-TAATACGACTCACTATAGGGCAGTAAACGCGGTTCTGCATG-3’ and 5’-CAGCATACAGGCCCAAGATC-3’) were transcribed using the Biotin RNA Labeling Mix (Roche) and T7 polymerase (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Probes were labelled using a nick translation reaction with the uridine analogues biotin-16-dUTP (Roche, CatNo 11093070910) or digoxigenin-11-dUTP (Roche ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... DIG-labeled nucleotides were detected using Anti-Digoxigenin-Fluorescein (sheep polyclonal, Roche, 1:75) incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche, Mannheim, BW, Germany) (1:1000 in PBST 5% fat-free milk ...
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bioRxiv - Genomics 2022Quote: ... The BAC clone (MSMg01-38O12) was labeled by nick-translation with biotin-16-dUTP (Cat# 11093070910, Roche Applied Science) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: For in vitro RNA transcription of LINC00941 the Sp6 RNA Polymerase was used according to the manufacturer’s instructions with the exception that LINC00941 was labelled with biotin-16-UTP (Roche). 2 µg of biotin-16-labelled LINC00941 were incubated with protein lysate of 3*107 keratinocytes ...
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bioRxiv - Genomics 2023Quote: ... accession numbers OY726585 and OY726586) were indirectly labeled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... rabbit anti-EGFR (Roche, #790-4347 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-GFP (Roche), anti-MAP1LC3B (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
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bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
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bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
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bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...
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bioRxiv - Genetics 2019Quote: ... rat polyclonal anti-Lid (1:500) [13] and mouse monoclonal anti-GFP (Roche #118144600001; 1:200). Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The AFS chamber was next functionalized with polyclonal anti-digoxigenin from sheep (Sigma, Roche cat. 11333089001) by injection of 50 μL of a 200 μg/mL anti-Dig solution (20 min incubation) ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used for immunostaining included anti-HA (1:500, ROHAHA rat polyclonal, clone 3F10, Roche) and anti-GFP (1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: LDH from rabbit muscle (Roche) was diluted to 5 µg/mL in 5-fold diluted boiled supernatants (for Figure 1E) ...
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bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the polyclonal murine anti N-terminal MSPDBL2 serum and a rat monoclonal α-HA antibody (Roche 3F10) were used ...
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Proto-sex locus in large yellow croaker provides insights into early evolution of the sex chromosomebioRxiv - Evolutionary Biology 2020Quote: ... The DNA probes constructed from a BAC clone containing dmrt1 (supplementary note 11) were labeled with biotin-11-dUTP using a Nick Translation System (Roche Diagnostics, Basel, Switzerland). The probes were added to the hybridization buffer ...
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bioRxiv - Genetics 2022Quote: ... 1µg of DNA was labeled with digoxigenin (for chromosome III) or biotin (for chromosome V) by nick-translation according to manufacturer instructions (Roche, 11745816910 and 11745824910).
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bioRxiv - Cancer Biology 2019Quote: ... Immunostaining was carried out using the BenchMark XT (Ventana Medical Systems, Illkirch Graffenstaden, France) with the OmniMap kit (a “biotin-free” system using multimer technology, Roche, Boulogne Billancourt, France) and a Tris borate EDTA pH8 buffer for antigen retrieval ...
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bioRxiv - Cell Biology 2023Quote: ... probe was amplified by PCR as described previously (Cox et al., 1993) and labeled using the biotin-nick translation mix (Roche Applied Science, # 11745824910), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... rabbit anti-Myc (Roche, 1:1,000); rat anti-HA (Roche ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged rhFGFb was detected using a primary anti-HISx6 mouse polyclonal antibody (Roche Molecular Systems, Inc., USA) or anti-FGF polyclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-GFP (1:1000; 11814460001, Roche). Secondary antibodies used were HRP-conjugated ...
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bioRxiv - Cancer Biology 2021Quote: ... PD-L1 (rabbit monoclonal, clone SP142, Roche) and CD163 (mouse monoclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-EGFR (prediluted, Roche, 790-4347), rabbit anti-TFF3 (1:250 ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by HRP-conjugated anti-rabbit (Roche) or anti-mouse (Santa Cruz ...
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bioRxiv - Biophysics 2022Quote: ... except that the carboxylic silica beads were replaced by polystyrene carboxylic beads and polyclonal anti-digoxigenin from sheep (11333089001, ROCHE) were used in our experiments.
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bioRxiv - Pathology 2019Quote: ... Ventana anti-Her2/neu(4B5) rabbit monoclonal primary antibody and anti-PR (1E2) rabbit monoclonal primary antibody were purchased from Roche Diagnostics GmbH ...
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bioRxiv - Neuroscience 2024Quote: Primary antibody incubation with rabbit anti-HA antibody was followed by incubation with OmniMap anti-rabbit HRP (05269679001, Roche Diagnostics). Fluorescence signal was detected with FITC fluorophore (07259212001 ...
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bioRxiv - Molecular Biology 2022Quote: ... and L-lactate dehydrogenase from rabbit muscle (Roche) was used as a standard ...