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Citations for Roche :
101 - 150 of 385 citations for B Raf Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Bottom Agar + Hygromycin B (Roche Cat#10843555001, 150 μg/mL).
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bioRxiv - Biochemistry 2023Quote: HDX of unbound and bound c-Met (1:2.2 ratio with mAb (Roche Diagnostics GmbH, Germany)) was performed by diluting c-Met into labelling buffer (20mM Histidine ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
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bioRxiv - Biochemistry 2020Quote: ... transfected with recombinant EMBacY BACs using X-tremeGENE DNA Transfection Reagent (Roche), and incubated for 72 h at 28 °C ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse I (Dnase I recombinant RNAse-free, Roche Diagnostics). Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA was removed by digestion using DNase I Recombinant (Roche, 04716728001) and RiboLock RNase Inhibitor (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant baculovirus was generated by initial lipofection with Xtreme gene reagent (Roche) of Sf21 insect cells (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... and basic fibroblast growth factor (bFGF, 10 ng/ml; recombinant bovine, Roche). The cells were then plated in a 96-well plate in complete neurosphere medium containing DMEM/F-12 EGF and bFGF ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x DNase buffer (500ul) and 200U recombinant DNase I (20ul, Roche, 04716728001) at 37 C in a shaker set at 100 RPM ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA contamination was removed through treatment with recombinant DNaseI (Roche Diagnostics, #04716728001) for 15 minutes at RT and column purification using Qiagen RNeasy Mini kit (#74106) ...
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bioRxiv - Neuroscience 2023Quote: ... DNA contaminations were eliminated with DNase I recombinant (#04716728001; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies used for IFA were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:200) (Roche); mouse anti-PMV mAb (1:50) ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Neuroscience 2021Quote: ... Slide were moved to a humid chamber and blocked with blocking solution (MAB 1×, blocking reagent (Roche) 2% ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was probed using the primary antibodies mAb mouse α-GFP (1:1’000, Roche Diagnostics #11814460001), mAb mouse α-PfGAPDH (1:20’000 ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 7.5) and placed in RBR buffer (20% Goat serum, 2% Roche Blocking Reagent in 1x MAB) on the orbital rocker for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... ADCC in no click or click mAbs conditions was evaluated using the Cytotoxicity Detection Kit (LDH, Roche) as instructed by the manufacturer.
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies used for IFA were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:200) (Roche); rabbit anti-GAP45 mAb (1:1000).
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bioRxiv - Evolutionary Biology 2020Quote: ... 25μl of 1X PCR buffer B (Kapa Biosystems, Boston, MA, USA) pre-warmed at 65°C was added then the legs were ground ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by mechanical dissociation and treatment with 0.1% collagenase B (Roche) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and ligated to KAPA single indexed adapters Set B (KK8702, Roche). Samples were processed according to manufacturer’s instructions with one exception ...
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bioRxiv - Developmental Biology 2023Quote: ... hiPSCs were selected with hygromycin B (50μg/ml; 843555001, Roche Diagnostics), puromycin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the transfected cells underwent initial selection with Hygromycin B (Roche, 10843555001) and were subsequently sorted into single colonies in 96-well plates via flow cytometry.
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bioRxiv - Plant Biology 2020Quote: ... 4 µg recombinant NAA50 was incubated with 100 µM Acetyl-Coenzyme A (Roche) in a 2X acetylation buffer (50mM Tris HCl pH 7.5 ...
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bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
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bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was then treated with DNase I recombinant RNase free (Roche) and the reverse transcription was done using the Super-Script IV (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... 100 μg of total RNA was incubated with recombinant DNase I (Roche, 04716728001) for 30 min at 37°C and the reaction was stopped by incubation at 75°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... Residual genomic DNA contamination was removed by treatment with recombinant DNAse I (Roche). cDNA was generated using Superscript II reverse transcriptase and Oligo d(T ...
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bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with 40 µL of primary antibody (1:1,000 rat anti-HA mAb 3F10, Roche) at 4°C overnight in a solution containing 3% BSA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IFAs were carried out on methanol-fixed cells using primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:100 and rabbit α-PfHP1 12 ...
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bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
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bioRxiv - Developmental Biology 2021Quote: Embryoid bodies (EBs) were generated from ESC lines using collagenase B (Roche) and cultured in base media (RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... tumors were excised and digested with 1 mg/mL collagenase B (Roche) and 0.02 mg/mL DNaseI (D5025 from Sigma ...
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bioRxiv - Genetics 2021Quote: ... and indexed with KAPA Single-Indexed Adapter Set A + B (Kapa Biosystems). Library quantification was by Qubit 1x dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... followed by the same perfusion buffer containing collagenase D and B (Roche) and protease XIV (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... stable transfectants were selected with 40 μg/mL Hygromycin B (Roche, 10843555001). cDNA and shRNA expression were induced by Doxycycline (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... CM plates supplemented with hygromycin B at 250 μg/ml (Roche, USA) or neomycin at 400 μg/ml (Amresco ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombination events were selected for with 200 μg/mL hygromycin B (Roche). Expression of BRCA1 variants was induced by the addition of 5 μg/mL doxycycline (Inalco ...
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bioRxiv - Immunology 2022Quote: ... 5% b-ME) supplemented with protease and phosphatase inhibitors (Roche/Sigma-Aldrich). A Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: In situ antibody stainings were done as described previously 31 using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-FLAG (M2 ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies used for western blots presented in this work were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:2,000) (Roche); mouse anti-GAPDH mAb (1:20,000) ...