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Citations for Roche :
251 - 300 of 1493 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... ground yeast powder was solubilized in 4 volumes of homogenization buffer (400 mM trisodium citrate, pH 8, 0.5% n-Dodecyl β-D-maltoside) and protease inhibitor cocktail (Roche). The soluble fraction was incubated with 10 μl magnetic beads (Dynabeads ...
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bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 4 mM β-mercaptoethanol, 10 mM ATP, 2.5 mM MgCl2, cOmplete EDTA-free protease inhibitor cocktail-Roche). The beads were eluted with NTA elution buffer (1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Mitotic chromosomes were resuspended in 40ml MNase buffer (20mM HEPES, pH7.7; 20mM KCl; 5mM β-mercaptoethanol; 1xProtease Inhibitors cocktail (EDTA free, Roche), 20mM β-glycerophosphate,1mM Sodium orthovanadate ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell pellet was resuspended in cold extraction buffer (10 mM Tris pH 7.5, 2 mM NaV, 50 mM NaF, 50 mM β-glycerophosphate, PhosSTOP Phosphatase inhibitor (Roche), cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... 25mM Tris-HCl pH=7.5, 1% Triton-X, 10mM Na3VO4, 40mM β-glycerophosphate, 10mM NaF and protease cocktail inhibitor from Roche). 1mg of total protein lysate was incubated with 1µg/mL of purified GST or GST-SidI overnight at 4°C in a rotary mixer ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were thawed on ice and suspended in 2 mL of lysis buffer A (50 mM NaPO4, pH 7.3, 300 mM NaCl, 2 mM β-mercaptoethanol, 20% glycerol and Roche cOmplete protease inhibitor (1 tablet per 10 mL)) ...
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bioRxiv - Cell Biology 2023Quote: ... A 145cm2 culture dish at 80% confluency was lysed in 600ul lysis buffer buffer (150mM NaCl, 25mM Tris pH 8.0, 20mM dodecylmaltoside, 5mM β-mercaptoethanol with cOmplete mini protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell pellet containing expressed untagged RAD15 was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 4mM EDTA, 20 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Genomics 2019Quote: ... the 5’ end of the Ppetra cDNA was determined with the 5’/3’ RACE kit 2nd generation (Roche). Reverse transcription was performed as recommended by the suppliers ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, Roche mini-tablet protease inhibitor ...
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bioRxiv - Immunology 2020Quote: Isolated B cells from Gal9KO mice were lysed in NP40 lysis buffer (50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM dithiothreitol, 5 mM EDTA, 0.1% Nonidet-P40, Roche cOmplete mini EDTA-free protease inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 mM NaF and protease inhibitors (Roche)] ...
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bioRxiv - Developmental Biology 2020Quote: ... and 5 μL/mL NBT (Roche, 11383213001), was then added and slides were placed at 37°C to allow colour to develop ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol and protease inhibitor cocktail (Roche). The cell debris was removed from the lysate by centrifugation at 16,000 rpm for 30min ...
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bioRxiv - Neuroscience 2020Quote: ... midazolam (5 mg/kg bodyweight; Dormicum, Roche), and medetomidine (0.5 mg/kg bodyweight ...
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bioRxiv - Cell Biology 2021Quote: ... in 5 mg ml−1 dispase (Roche) and 0.1 mg ml−1 DNase I (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and 5 μg of RNase A (Roche) per mg of cross-linked complex and incubated at 52 °C for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μl of SybrGreen master mix (Roche) and 1 μl of water were processed in a LightCycler® 480 instrument (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... 5% bovine serum albumin (BSA; #10735086001, Roche)] rotating overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 5% bovine serum albumin (BSA; #10735086001, Roche)] rotating overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol and 1x protease inhibitor (Roche)] ...
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bioRxiv - Genetics 2024Quote: ... 5 μg/ml DNAse I (Roche 10104159001), and 0.05%Trypsin (Gibco 25200056 ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked in 5% BSA (Roche, 10735086001) in PBS solution ...
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bioRxiv - Cancer Biology 2020Quote: ... HPV DNA capture was performed with NimbleGen’s SeqCap EZ Custom Library containing probes for all HR HPV types (Roche NimbleGen, Inc.,) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested either for 1 hour at 37°C in a digestion mix of 3 mg/ml collagenase type A (Roche, 11088793001) and 25 μg/ml DNAse (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Genetics 2023Quote: ... We performed copy number qPCR using genomic DNA from three humanized and three wild type individuals using Light Cycler 480 SYBR Green I Master Mix (Roche #04707516001). The biological replicates for each individual were run in triplicate and error bars show the standard deviation from these technical replicates ...
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bioRxiv - Physiology 2023Quote: ... Then the heart was perfused with the same solution containing 1 mg/mL collagenase Type A (Catalog # 9036-06-0; Roche, Germany) and 0.08 mg/mL protease Type XIV (Cat log # 48655321 ...
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bioRxiv - Cancer Biology 2019Quote: ... the cell pellets were resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 % NP-40, 5 mM MgCl2, 5 % glycerol and protease inhibitor cocktail [Roche]) and incubated for 20 min at 4 °C with end-over-end rotation ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche cOmplete EDTA-free protease inhibitor catalog # 11872580001) ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 150 mM NaCl, 5 mM EDTA, 5% glycerol, 1% Triton X-100, and 1x complete protease inhibitor [Roche]). Cleared lysates were then incubated with glutathione sepharose (GE healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... with 0.5μM of each primer (Forward: 5’-GGGAGCCTGATCCTATCGTT-3’; Reverse: 5’-TCCCAAAGCACAGCTTCC-3’) and 50nM Universal ProbeLibrary Probe #67 (Roche). RT-PCR was performed in QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.25 μl forward (5 μM, IDT) and 0.25 μl reverse primer (5 μM, IDT) and was analysed on a LC480 instrument (Roche).
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bioRxiv - Cancer Biology 2021Quote: ... 0.25 μl forward (5 μM, IDT) and 0.25 μl reverse primer (5 μM, IDT) and was analyzed on a LC480 instrument (Roche).
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bioRxiv - Genetics 2021Quote: ... and the cell pellet resuspended in cold lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 1% Triton X-100, cOmplete protease inhibitor cocktail, Roche) and incubated on ice for 30 minutes ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... resuspended in 10 ml buffer A (50 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM MgCl2, 5 mM ATP, Roche complete EDTA-free protease inhibitor ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: ... blocked in 5 % milk and probed with α-GFP (1:1,000 in 5 % milk; Roche Diagnostics GmbH, Mannheim, Germany), for GFP-based sensor probing ...
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bioRxiv - Biochemistry 2023Quote: ... after removal of the medium cells were lysed in 1 mL of lysis buffer (50 mM Hepes [pH 8.0], 150 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 % Glycerol, supplemented with 0.55 % Nonidet P40 substitute [Roche] and cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche]) ...
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bioRxiv - Biochemistry 2019Quote: ... 1 % Tween-20 and 1 mM β-mercaptoethanol supplemented with 1 mg/mL lysozyme and protease inhibitor cocktail tablet (Roche, Switzerland) at 4°C ...
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bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
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bioRxiv - Microbiology 2020Quote: ... 20 uL was treated with β-Glucuronidase/Arylsulfatase obtained from Helix pomatia according to the manufacturer’s recommendation (Roche Catalog No. 10127698001). Liver was extracted by homogenizing a piece of liver with 1 mm stainless steel beads at 30 Hz for 5 minutes in methanol + 0.1 % acetic acid ...
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bioRxiv - Microbiology 2020Quote: ... ifn-β and bcl-2 genes were amplified with specific primers and quantified by qPCR using a LightCycler 96 system (Roche). The mRNA levels of il-6 ...
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bioRxiv - Plant Biology 2022Quote: ... and 1X protease inhibitor cocktail) with a combination of phosphatase inhibitors (20mM NaF, 1mM Na2MoO4, 10mM Na3VO4, 50mM β-glycerophosphate and 1X PhosStop cocktail (Roche) to preserve EIR1 phosphorylation status ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM β-mercaptoethanol and 20 μM GDP supplemented with DNase I (10 μg/ml) and cOmplete protease inhibitor cocktail tablets (Roche). Cells were mechanically disrupted using a Microfluidizer (Microfluidics International Corporation ...