Labshake search
Citations for Roche :
301 - 350 of 740 citations for 9 Z 11 E 13 Z OCTADECATRIENOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 150-300 ng of indicated construct using XtremeGene 9 transfection reagent (Roche) according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2021Quote: Transfection of CRISPR/Cas9 constructs was performed using X-tremeGENE 9 DNA Transfection Reagent (Roche), with 4.0 μg plasmid per 200.000 cells/well in a six-well plates ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 µg sgRNA plasmid using X-tremeGENE™ 9 DNA transfection reagent (Roche 06365809001). One day after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using the X-treme GENE 9 DNA transfection reagent (Roche Applied Science) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Nup96-GFP KI U2OS cells were transfected using X-tremeGENETM 9 DNA Transfection Reagent (Roche), and 24h after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were deparaffinized followed by antigen retrieval in CC1 buffer (pH 9, 95°C; Roche), endogenous peroxidase blocking ...
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bioRxiv - Biochemistry 2024Quote: ... A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche, XTG9-RO) was added to the mixture prior to incubation and application on HEK293T cells using standard methods 1 ...
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bioRxiv - Synthetic Biology 2019Quote: The SPO-13-AM1 oligo still on beads was poly adenylated using TdT (Roche, Santa Clara, CA) and Duplase-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... All libraries and controls went through 13 PCR cycles using KAPA HiFi HotStart Ready Mix (KAPA Biosystems). PCR products were size-selected on a 1.8% agarose gel before loading on a Novaseq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... and LacZ (β-D-galactosidase of E. coli (Hoffmann-La Roche, Basel, Switzerland) were used ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% deoxycholic acid and protease inhibitors (Roche), and thrice with lysis buffer without detergent ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.5% fatty acid-free BSA (Roche), 10 mg ml-1 Collagenase D (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% deoxycholic acid with protease inhibitors (Roche). The lysates were subject to anti-GFP immunoprecipitation using GFP-Trap beads or anti-Myc immunoprecipitation using Myc-Trap beads (ChromoTek) ...
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bioRxiv - Plant Biology 2023Quote: ... ethylenediaminetetraacetic acid free protease inhibitor mixture (Roche) and phosphatase inhibitor mixture (Sigma-Aldrich) ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... The NTP labeling mix contained either DIG-11-UTPs (DIG RNA Labeling Mix, Roche; Cat. No. 1277073) for synthesis of DIG-labeled probes or Fluorescein RNA Labeling Mix (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910). Nick-translated DNA was precipitated using ethanol precipitation and resuspended in hybridization mix (50% deionized Formamide + Master mix containing 10% Dextran Sulphate ...
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bioRxiv - Genomics 2020Quote: Northern probes were first synthesized using the PCR DIG Probe Synthesis Kit (Roche # 11 636 090 910). The 512 bp frq probe was amplified from wild-type Neurospora genomic DNA with primers ...
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bioRxiv - Genomics 2020Quote: ... subsequent washes were performed using the DIG Wash and Block Buffer Set (Roche # 11 585 762 001). Anti-Digoxigenin-AP Fab fragments were used at 1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... for Kcns3 and Kcnb1 probes or in the presence of digoxigenin (DIG)-11-UTP (Roche, Mannheim, Germany) for Pvalb probe ...
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bioRxiv - Cell Biology 2021Quote: ... Assay was performed according to protocol in Cell proliferation kit II (XTT) (Roche, #11 465 015 001).
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bioRxiv - Developmental Biology 2022Quote: ... DNA probes were labelled by nick translation with either biotin-16-dUTP or digoxigenin-11-dUTP (Roche). For simultaneous IF and RNA FISH ...
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bioRxiv - Cell Biology 2022Quote: ... the following antibodies were used (all at 1:3000 dilution): α-GFP (Roche, 11 814 460 001), α-Pgk1 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The labelling nucleotide mix contained either biotin-16-dUTP or digoxigenin-11-dUTP (both Roche, Mannheim, Germany). Due to its long size ...
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bioRxiv - Genomics 2021Quote: ... probes were generated by PCR incorporation of DIG-11-dUTP into target sequences following manufacturer’s instructions (Roche). Chromosome 5-chromosome 7 translocation was detected using primers AB1028 and AB1029 amplifying a 180 bp region of chromosome 5 (Chr5 nt ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoblotted with a mouse monoclonal anti-HA antibody (clone 12CA5; Roche, 11 583 816 001, RRID:AB_514505). Rabbit monoclonal anti-PSTAIR (anti-Cdc2 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 9 µl of qPCR reaction mix containing 5 ul LightCycler 480 Probes Master (Roche, Cat#04707494001), 3.4 ul of Nuclease Free H2O (Ambion ...
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bioRxiv - Cell Biology 2022Quote: ... The construct was then sequenced and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and GFP positive single cells were sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Molecular Biology 2022Quote: ... structure in the 5’UTR and 70 ng of firefly luciferase (FLuc)-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... or a HP50 hairpin structure in the 5’UTR and 2 ng of FLuc-expressing normalizer plasmid (pcDNA5_FRT_TO_EGFP_AID_Luciferase, a gift from Andrew Holland) using X-tremeGENE 9 DNA transfection reagent (Roche, 06365787001) at a ratio of 5 µL reagent to 1 µg DNA to 100 µL Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Lipofection: Plasmid was lipofected into cells using X-tremeGENE 9 DNA Transfection Reagent (Roche; Basel, Switzerland). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Bioengineering 2023Quote: ... Transfections of HEK293T and 293FT cells were performed using X-tremeGENE 9 DNA Transfection Reagent (Roche). Murine Stem Cell Viruses (MSCV ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: Cells were treated with DMSO or 0.5 μM dTAG-13 for 18 h and subsequently arrested at mitosis with 0.1 mg/ml colcemid (Roche) for 6 h (total 24 h) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were thawed on ice and disrupted in lysis buffer (100 mM Tris-HCl pH 7.4, 0.5% Triton X-100, 150mM NaCl, 13% w/v glycerol, protease inhibitor tablet, Roche) by bead beating (mini bead beater-16 ...
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bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
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bioRxiv - Genomics 2019Quote: ... we used Escherichia coli k-12 strain MG1655 (hereafter E. coli; Roche, Basel, Switzerland), which has a high-quality genome sequence available (GenBank accession NC_000913 ...
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bioRxiv - Microbiology 2019Quote: Separated RNA was transferred from polyacrylamide gels to positively charged nylon membranes (Roche, cat. 11 209 272 001) using the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-GFP antibodies (11–814–460-001; Roche) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Cell Biology 2019Quote: Antibodies used for immunofluorescence (IF) experiments in this study were: HA tag (Roche, 3F10/11 867 423 001), giantin (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... DIG-labeled RNA probes (prepared according to the protocol provided by Roche, Cat. No. 11 277 073 910) or radiolabeled DNA probes and RNA probes were used (Table S5) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with phosphatase inhibitor (04-906-837001) and protease inhibitor (11-836-153001s) (both from Roche, Mannheim, Germany) to generate whole-cell extracts (WCEs) ...
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bioRxiv - Genomics 2022Quote: ... Cycle Pure Kit (Omega) and then labelled with either biotin–16-dUTP or digoxigenin–11-dUTP (Roche Diagnostics) using the BioPrime Array CGH random priming kit (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... purpureum gDNA probe was labeled with digoxigenin-11-dUTP (DIG) using a DIG Nick Translation Kit (Roche Diagnostics). Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics) ...
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bioRxiv - Evolutionary Biology 2021Quote: The PCR products of the NgGspE and NgGspF genes were labelled by alkali-stable digoxigenin-11-dUTP (Roche) using DecaLabel DNA Labeling Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: Cellular ATP concentrations were determined using the ATP Bioluminescence Assay Kit CLS II (11 699 709 001, Roche) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Fosmid and BAC clones (BACPAC Genomics) were labelled by nick translation with digoxigen-in-11-dUTP (Roche, #11093070910) or biotin-16-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... The DNA probes were 3′ end-labelled using digoxigenin (DIG)-11-ddUTP and terminal transferase (Roche, Basel, Switzerland). Binding of TFRs to DNA was analyzed using the DIG Gel Shift Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated in 2 ml HBSS solution containing 2.5 mg/ml collagenase A (Roche, 11 088 793 001) and 10 mg/mL pancreatin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with DNase and one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche #11 873 580 001). Cells were lysed using a French press (2x ...