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Citations for Roche :
301 - 350 of 6461 citations for 8H Purin 8 one 1 7 dihydro 6 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8) supplemented with Proteinase Inhibitor Cocktail (Roche, 11697498001) and chromatin was sonicated using the Bioruptor pico (Diagenode ...
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bioRxiv - Biophysics 2023Quote: ... pH 7) with addition of cOmplete tablets EDTA-free (04693132001, Roche, Switzerland), 0.5 mM Na3VO4 ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Plant Biology 2022Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Cell Biology 2022Quote: ... the dorsal skin of C57BL/6 neonates was collected and incubated overnight with 1 mg/mL collagenase/dispase (Roche). The dermis and epidermis were separated by incubating the skin with 0.25% trypsin/10 mM EDTA in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a 6 μl volume of 1 x Kapa Probe Fast Mastermix (Kapa Biosystems, Amsterdam, Netherlands). TaqMan assays (FAM labelled ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were cotransfected with 4 μg of Env-deficient HIV-1 proviral plasmid (Q23ΔEnvGFP) and 2 μg of HIV-1 Env clone of interest using Fugene 6 transfection reagent (Roche) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... testis albuginea was peeled and added in RIPA buffer supplemented with 1mM phenyl methyl sulfonyl fluoride (PMSF) and PIC (Roche Diagnostics, 04693132001), the solution was sonicated transiently and then on the ice for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... 8 mM EDTA) with Complete™ protease inhibitor cocktail (Roche). The Triton X-100-insoluble material was pelleted twice by centrifugation (10 min at 16,000 g) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and phenol (phenol-Tris saturated, pH 8; Roche, Indianapolis, IN) and chloroform:isoamyl alcohol (American Bioanalytical ...
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bioRxiv - Biochemistry 2022Quote: ... pH=8) supplemented with EDTA-free protease inhibitor mixture (Roche) and 100 mM PMSF ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Biophysics 2023Quote: Microchambers were incubated with 8 mg/µl anti-digoxigenin (Roche) at 25°C for 90 min and then the blocking buffer (PBS with 1% α-casein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 106 cells were lysed for 15 min on ice in 100 μL pre-chilled lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche, Basel, Switzerland]). Lysates were pelleted at 20,000 x g for 15 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl, 20 mM Tris-HCl pH 8, 2.5 mM EDTA, 10 % glycerol, 1 % Triton, 1x of PhosSTOP, 1x Roche protease inhibitor mixture) and given 20 strokes with the Dounce Homogenizer ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated by isopropanol precipitation of cells and tumor lysates (lysis buffer was Tris [pH 8] 100 mM, EDTA 5 mM, SDS 0.2%, NaCl 200 mM, and 1 mg/mL proteinase K [Roche Diagnostics, Mannheim, Germany]) and resuspended in Tris/EDTA (TE ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) supplemented with one pellet EDTA-free protease inhibitor (Roche), and sonicated on ice for 5 × 30 s at 80% amplitude (0.5 s cycles ...
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bioRxiv - Genomics 2019Quote: ... one cOmplete EDTA-free EASYpack protease inhibitor tablet (Roche, Basel, Switzerland) was added to each cell pellet corresponding to 1 L of growth ...
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bioRxiv - Neuroscience 2019Quote: ... 50 mM ammonium bicarbonate and one complete mini protease inhibitor (Roche). The lysate was sonicated on ice using Bioruptor-Diagenode and centrifuged at 2500 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... One tablet of Roche cOmplete protease inhibitor was dissolved (Roche, 11697498001) in 50 mL of DI water to obtain a working solution of 50x protease inhibitor cocktail ...
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bioRxiv - Microbiology 2020Quote: ... One gel was blotted to a PVDF (Polyvinylidene fluoride) membrane (Roche) by semi-dry Western blotting ...
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bioRxiv - Biophysics 2022Quote: ... one crushed tab of Complete EDTA-free protease inhibitor cocktail (Roche) was added to ice-cold extraction buffer ...
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bioRxiv - Microbiology 2022Quote: ... and a protease inhibitor cocktail (Roche, one tablet/10 mL buffer)) ...
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bioRxiv - Biochemistry 2021Quote: ... one tablet per 50 mL of buffer (EDTA-free, Roche Diagnostics) and benzonase (E1014-25KU >250 Units/liter of culture)) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and one tablet of EDTA-free protease inhibitor (Roche) per 50 mL ...
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bioRxiv - Biochemistry 2021Quote: ... 10% (v/v) glycerol and one Complete protease inhibitor tablet (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... one Complete EDTA-free protease inhibitor cocktail tablet (Roche, Basel, Switzerland) and 1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM ATP) using one Protease Inhibitor Cocktail Tablet (Roche) per 100 mL of the lysate ...
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bioRxiv - Microbiology 2022Quote: ... and one complete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Cells were homogenized by inverting the tube several times until the mixture becomes very viscous (a 5 min incubation period at room temperature might be necessary to achieve viscosity) ...
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bioRxiv - Plant Biology 2022Quote: ... one tablet of cOmplete EDTA-free Protease Inhibitor Cocktail /50ml (Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... one mM EDTA and supplemented with protease and phosphatase inhibitors (Roche)] ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 % glycerol) supplemented with one tablet of protease inhibitor cocktail (Roche) for every 10 mL of RIPA ...
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bioRxiv - Biochemistry 2023Quote: ... with one tablet of protease inhibitors (cOmplete Protease Inhibitor Cocktails, Roche) and 500 µl of 2mg/ml DNaseI (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... one EDTA-free mini-Complete protease inhibitor tablet (Roche, Basel, Switzerland) and DNase I ...
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bioRxiv - Biochemistry 2023Quote: ... aprotinin and one tablet complete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with complete protease inhibitors (one tablet per 25 mL: Roche), and 0.1% b-mercaptoethanol (Sigma) ...