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Citations for Roche :
201 - 250 of 309 citations for 8 Hexadecyne since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Probes were generated using primers listed in Supplementary Table 1 from the G135P65476A4 BAC as described in [8] and were labeled using the DIG-Nick Translation Mix (Roche) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Cell Biology 2019Quote: ... washed in PBS, and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM EDTA at pH 8, 10% glycerol, 1 mM DTT, 0.5 mM PMSF, 0.1 mM sodium orthovanadate, and 1X Roche protease inhibitors). Collected nuclear pellets were lysed in in 1× RIPA buffer (10 mM Tris-Cl at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: The HEK293 cell pellet was resuspended in 25 mL IEX buffer A (50 mM Tris, pH 8) with protease inhibitors (cOmplete, EDTA-free cocktail, Roche) and sonicated 20 s on ...
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bioRxiv - Cell Biology 2019Quote: ... The qPCR reaction was carried out in 8 μl with a primer concentration of 1 μM and SYBR Green Master mix (Roche) in a Roche LightCycler 480 system ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Genomics 2022Quote: ... Each ligation product was index-amplified using unique dual 8-bp indexing primers for eight cycles with KAPA HiFi polymerase (Roche).
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bioRxiv - Genomics 2022Quote: ... The cell pellet was lysed in cell lysis buffer (20 mM Tris-HCl pH 8, 85 mM KCl, 0.5% NP-40) supplemented with 1X protease inhibitors (Roche, 11836170001) on ice for 20 minutes then spun at 5000 rpm for 10 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... then ground in 500 μl freshly prepared lysis buffer (25 mM Tris HCl pH 8, 150 mM NaCl, 1% SDS, 1x cOmplete ULTRA protease inhibitor (Roche), and 1x PhosSTOP (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 8-keto-NeuAc derivatives for 24 h and proliferation measured using WST-1 (Roche Applied Science) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 50 ml lysis buffer (Hepes 50 mM pH 8, 500 mM NaCl, 10 mM Imidazole, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) at 4°C and sonicated ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was centrifuged at 20000 g for 30 min at 4°C and the resulting pellet was solubilized with lysis buffer (20 mM Tris–HCl pH 8, 150 mM NaCl, 1% TritonX100, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2023Quote: ... A total amount of 1010 bacteria were pelleted at 4°C and resuspended in 1 mL of buffer A (20 mM Tris-HCl pH 8, 200 mM MgCl2) supplemented with protease inhibitor cocktail (PIC, Roche) and lysozyme to a final concentration of 0.5 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... were lysed by sonication in ∼140 mL lysis buffer (50 mM Tris-HCl pH 8, 250 mM KCl, 1 mM TCEP) supplemented with protease inhibitor cocktail (Roche) and DNase I (5 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Synthetic Biology 2023Quote: ... The second strand synthesis reaction was performed with ∼50 ng (IDT) or ∼8 ng (Twist) of template DNA and KAPA HiFi polymerase (Roche) with the annealing temperature at 53°C and extension time of 30 sec for 2 (IDT ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... The bacterial pellets were dispersed in lysis buffer of 8 M urea in 100 mM ammonium bicarbonate and protease inhibitor cocktail (CoMplete, (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Biochemistry 2023Quote: ... the cell pellets resuspended in lysis buffer (purification buffer 50 mM Tris pH 8, 50 mM NaCl, 2 mM DTT; + cOmplete EDTA-free Protease Inhibitor (Roche)) and lysed by sonication ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 30 mL of 40 mM Tris pH 8 supplemented with 1 tablet of EDTA-free protease inhibitor tablet (Roche), and 0.1 mM PMSF ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were lysed in equilibration buffer (20 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8) supplemented with lysozyme and anti-protease cocktail (Roche), followed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen cell pellets were resuspended in 300 μL of cold hypotonic buffer (10 mM Tris-HCl pH 8, 1.5 mM MgCl2, 1 mM KCl, 10X PhosphoSTOP (Roche, 4906845001), 2X Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... and twice with TE wash buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 1x Roche protease inhibitor mixture).
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bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Immunology 2023Quote: ... The cell pellets were treated with lysis buffer (50 µg/mL digitonin, 75 mM NaCl, 1 mM NaH2PO4, 8 mM Na2HPO4, 250 mM sucrose, and Roche cOmpleteTM protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were disrupted in 1% NP-40 lysis buffer (140 mM NaCl, 10 mM Tris-HCl pH 8, 1% NP-40) supplemented with proteinase inhibitors (Roche). Proteins were separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Cell Biology 2020Quote: ... cells were collected via centrifugation and resuspended in lysis buffer (40mM Tris pH 8, 300mM NaCl, 10% glycerol, 10mM imidazole, 1mM DTT, 1mM PMSF and 1x Roche PIC). Cells were lysed by sonication and after clearing the lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in lysis buffer (100 mM Tris pH 8, 300 mM NaCl, 5ml Mg2Cl, 1mM ß-mercaptoethanol and protease inhibitor tablets (complete tablets, Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed (10mM Tris-HCl [pH 8], 0.25% Triton X-100, 10mM EDTA, 100mM NaCl, Roche 1X Complete Protease Inhibitor) and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS ...
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bioRxiv - Genomics 2019Quote: We amplified 5 ng of the HSS library with the following primers: STARR-Seq-AG-f and STARR-Seq-AG-r (Supplemental Table 8) using KAPA HiFi 2x Readymix (Kapa Biosystems) with a 65 °C annealing temperature and 30 s extension ...
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina compatible adapters (IDT, Inc) were ligated to the mate pair fragments and amplified with 8-12 cycles of PCR (KAPA Biosystems).