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Citations for Roche :
101 - 150 of 1414 citations for 8 Chloro pyrido 3 4 b pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Immunology 2021Quote: ... washed in FACS buffer (4% FBS, 3 mM EDTA, and 40 μg/ml DNAse I (Roche)) and incubated with combinations of antibodies to CD45 (30-F11) ...
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bioRxiv - Immunology 2019Quote: ... 2.93mg/ml collagenase B (Roche, 11088815001) and 1.9mg/ml DNase (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... and B (1.8 mg/mL, Roche) supplemented with 1% Penicillin/Streptomycin for 12 hours at 37 °C using end-over-end shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... 1mg/mL Collagenase B (Roche 11088815001), 4U/mL Hyaluronidase (Sigma H3506) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... hygromycin B was purchased from Roche Applied Science ...
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bioRxiv - Cancer Biology 2020Quote: ... 80 µl of dephosphorylation solution (8 μl 10x dephosphorylation buffer, 2 μl RNasin Plus, 67 μl ddH2O, and 3 μl alkaline phosphatase (Roche, catalog no. 10713023001)) was added to the beads ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Microbiology 2020Quote: ... Sections were stained with NBT (Nitroblue tetrazolium) and BCIP (1,5-bromo-4-chlooro-3-indolyl-phosphate, Roche, Switzerland) in developing solution at 37°C for 2 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were incubated at 37°C for 3-4 h before adding DNase I (0.1 U/µl) (Roche) and incubating for another 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were dissociated using collagenase B (Roche) and cultured in low cluster plates to allow EB formation in in RPMI 1640 base media (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... 4.6 mg Collagenase B (Roche, Basel, Switzerland) and 2 mg/ml Actinomycin D (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were dissociated using collagenase B (Roche) and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Collagenase B (CAT No: 11088815001) from Roche; CsCl (CAT No ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to 3-4 ml of digestion media containing 0.1 mg/ml Liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (3–4 μg) was used to prepare cDNA with the Transcriptor First-Strand cDNA synthesis kit (Roche). qPCR was performed with TaqMan gene expression assay primers (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 million cells were pre-extracted on ice with ice-cold 30 µl CSK buffer (25 mM HEPES pH 7.4, 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 300 mM sucrose, 0.2% Triton X-100, 1 Roche cOmplete protease inhibitor cocktail tablet per 50 ml of buffer ...
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bioRxiv - Biochemistry 2022Quote: ... was from Roche (9048-46-8). Poly-D-lysine and LiCl were from Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... 8 mg/mL collagenase P (Roche) was added to the trypsin mixture for thorough digestion ...
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bioRxiv - Microbiology 2019Quote: ... and the ketoreductase-B domain of the mycolactone polyketide synthase genes (KR-B) [22] using RT-PCR reagents from Roche PCR Kit (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 1.5 mg/mL of collagenase B (Roche) and 1 mg/mL of the protease dispase II (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Hygromycin B and protease inhibitors were from Roche. Odyssey Blocking Buffer was from LI-COR ...
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bioRxiv - Genomics 2022Quote: ... and 1 mg/mL Collagenase B (Roche, #11088815001) supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco ...
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bioRxiv - Genomics 2022Quote: ... and 1 mg/mL Collagenase B (Roche, #11088815001) supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and 250 µg/mL of Collagenase B (Roche) to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2023Quote: ... and 250 μg/mL hygromycin B (Roche Diagnostics) 48 h after PEI transfection ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were incubated for 3 h on a spinning wheel at 4°C with 40 µl of Protein G Sepharose beads (Roche) and 2.5 µg of the specific BTV-NS3 antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Analysis was performed on either an LSRII 3 or 4-laser flow cytometer (Becton Dickinson) or Attune Acoustic Flow Cytometer (Roche). Post analysis was performed using either FACSDiva (BD) ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was hybridized with a TAA(CCCTAA)4 probe which was conjugated with digoxigenin (DIG oligonucleotide 3⍰-end labelling kit, Roche) and signal was revealed using the anti-DIG-alkaline phosphatase antibodies (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... SOS1•RAS complex was formed by incubating SOS1 and RAS at a stochiometric ratio of 1:3 overnight at 4° with 20 mM EDTA and alkaline phosphatase (Roche). The complex was then purified by gel filtration ...
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bioRxiv - Neuroscience 2021Quote: ... pH 8) containing phosphatase (PhosSTOP tablets, Roche) and protease inhibitor (Complete Tablets ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8 and complete protease inhibitor (Roche) were then added to the cells suspension and incubation continued 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... or in 1.5 U/mL of collagenase B (Roche) at 37°C for 18 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... and NimbleGen SeqCap Adaptor Kit A or B (Roche) and sequenced by Illumina NextSeq 500 (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... pH 7.4) with 1 mg/ml Collagenase B (Roche). Kidneys were harvested and thin tissue slices along the cortical-medullary axis were obtained and proceeded to digestion ...
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bioRxiv - Biophysics 2022Quote: ... and collagenase B (Cf = 0.54 U/ml, Roche 11088807001). The enzymatic digestion of the tissue was carried out for 30 min in a 37°C water bath ...
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bioRxiv - Biochemistry 2022Quote: ... A solution containing 0.7 mg/ml Collagenase B (Roche), 3 mg/ml Dispase II (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were dissociated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... SeqCap Adapter Kit A and B (Hoffmann-La Roche), and DNA purification beads ...
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bioRxiv - Immunology 2022Quote: ... 0.1% amphotericin B and 0.15% pronase (10165921001, Roche Diagnostic). The single cell suspension was harvested and stained to identify BCAMhi and BCAMlo BCs ...
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bioRxiv - Systems Biology 2022Quote: ... pH 7.4) with 1 mg/ml collagenase B (Roche). We harvested the kidneys ...
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bioRxiv - Cell Biology 2023Quote: ... and hygromycin B (0.3 mg mL-1, Roche, 10843555001). All cell lines were cultured in a humidified incubator at 37°C ...