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Citations for Roche :
1 - 50 of 7140 citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Systems Biology 2021Quote: ... 2% fatty acid-free bovine serum albumin (BSA) fraction V (Roche), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Plant Biology 2022Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Genomics 2019Quote: ... 5.6mmol/L glucose with 2% BSA fraction V fatty acid free (Roche Diagnostics), 50μmol/L 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... Basal respiration was measured in DMEM containing 2% fatty-acid-free BSA (Roche). Cells were treated with 100 μM isoproterenol to stimulate respiration ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2% bovine serum albumin (BSA; fraction V, fatty-acid-free; Roche), 50 µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... except that the carboxylic silica beads were replaced by polystyrene carboxylic beads and polyclonal anti-digoxigenin from sheep (11333089001, ROCHE) were used in our experiments.
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bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Genetics 2019Quote: ... cells were vortexed and passed through a syringe in ubiquitin lysis buffer (2% SDS, 150mM NaCl, 10 mM Tris HCl pH 8, 1 Roche protease inhibitor tablet ...
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Biophysics 2021Quote: ... 2 μg ml−1 DNaseI (Roche), and protease inhibitor cocktail (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM MgCl2, 2 mM DTT, 2 µM leupeptin, 2 mM PMSF, 250 mM sucrose, and 1× PhosSTOP phosphatase inhibitors [Roche]). The homogenate was centrifuged for 15 min at 10,000 × g at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 1% Triton X-100, 2 mM EDTA, 2 mM EGTA, 1 mM PMSF, and complete protease inhibitor cocktail tablet; Roche), incubated with continuous shaking at 4°C for 20 min and then centrifuged at 12 000xg at 4°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... 2% Triton X-100) containing ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche cOmplete, Sigma Aldrich)] ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2 μg ml−1 collagenase A (Roche), 2.4 U ml−1 dispase I (Roche) ...
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bioRxiv - Immunology 2021Quote: ... containing 2 mg ml-1 collagenase/ dispase (Roche) and 0.2 mg ml-1 DNase I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... and 10 U mL−1 IL-2 (Roche) for 48 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...
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bioRxiv - Biochemistry 2023Quote: ... and were resuspended in 30 mL lysis buffer 1 (50 mM sodium phosphate, 300 mM NaCl, pH 8) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension three times through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Cell Biology 2021Quote: ... 350 mM NaCl, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 0.1% Triton X-100, 0.05% 2-ME, 1x Roche protease inhibitors ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate, 50 mM NaF, 2 mM EDTA, 2 mM DTT, 0.2 mM Na orthovanadate, 1 X Roche protease inhibitor #11836170001). Lysates were sonicated and centrifuged to remove debris ...
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bioRxiv - Immunology 2023Quote: ... human interleukin-2 (IL-2) (10 IU/ml, Roche), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2019Quote: ... rhIL-2 (200 U mL−1; Roche Applied Science) and rhTGF-β1 (1 ng mL−1) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT and 1 × cOmplete protease inhibitors (Roche) and sonicated for 15 minutes total time (10s on/20s off ...
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bioRxiv - Microbiology 2022Quote: Fresh pre-cut pancreatic tissue (2 × 2 mm) were digested with a solution of collagenase P in HBSS-1% HEPES (0.75 mg/mL, Roche) at 37°C for 7 min with shaking ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
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bioRxiv - Microbiology 2021Quote: ... 2 M CaCl2 and 2 UU/ml DNAse I (Roche) for 30 min at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Biophysics 2021Quote: ... 2 mM MgCl2) supplemented with 2 protease inhibitor tablets (Roche), 1 mM PMSF ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After incubation for 1-2 hours in a 1% blocking solution (Roche) dissolved in 1× PBS ...
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bioRxiv - Biochemistry 2019Quote: ... washed with PBS and suspended in Ni-wash buffer (20 mM Tris/HCl pH 8, 10 mM imidazole, 200 mM NaCl, 10 mM DTT, 2 mM PMSF, 0.5x Roche Protease Inhibitor cocktail ...
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Cancer Biology 2020Quote: ... grade 2 (Roche), homogenizing for 20min with a plastic Pasteur pipette ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) with 2% complete protease inhibitor (Roche) for 15 min on ice ...