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Citations for Roche :
151 - 200 of 310 citations for 8 Hydroxyethylamino adenine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... product KK8504),with palindromic forked adapters using unique 8-base index sequences embedded within the adapter (purchased from Roche). The libraries were then amplified by 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in cold lysis buffer (10 mM Tris pH 8, 10 mM NaCl, 0.2% NP-40, 1x protease inhibitors (Roche)) and incubated for 20 min on ice ...
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bioRxiv - Systems Biology 2022Quote: ... 6 mM MgCl2, 1 mM EDTA, 100 mM NaCl, 0.1% Tx-100, pH 8, and 1× Protease Inhibitor Cocktail, Roche) at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... and extracted in 4 ml of freshly prepared Extraction Buffer (50 mM HEPES pH 8, 150 mM NaCl, 0.3% NP-40, 1x cOmplete ULTRA protease inhibitor - EDTA (Roche), 1mM PMSF ...
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bioRxiv - Plant Biology 2022Quote: ... PHB variant complexes were captured using 25 μl Protein A Dynabeads and washed 4x with freshly prepared Wash Buffer (50mM HEPES pH 8, 450mM NaCl, 0.1% NP-40, 1x cOmplete ULTRA protease inhibitor - EDTA (Roche), 1mM PMSF ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old tmk1-1 roots expressing TMK1::TMK1-FLAG were grounded to powder in liquid nitrogen and homogenized in ice-cold sucrose buffer (20 mM Tris–HCl pH 8, 0.33 M sucrose, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche)) ...
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bioRxiv - Physiology 2022Quote: ... and resuspended in 600 μL of cold nuclear lysis buffer (10 mM Tris pH 8, 1 mM EDTA, 0.5 mM EGTA, 0.3% SDS, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 min followed by post-coloration using Bluing reagent for 4 min at RT (Roche Diagnostic, Meylan, France). The slides were then dehydrated (ethanol and xylene ...
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bioRxiv - Physiology 2023Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche). The final libraries were quantitated with Qubit (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... containing 0.1 mM CaCl2 and 1.0 mM MgCl2 and lysed in 8 urea with Complete protease inhibitor (Roche 11245200) and/or Halt phosphatase inhibitor (Pierce A32957 ...
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bioRxiv - Cell Biology 2024Quote: ... or ice-cold 8% (v/v) paraformaldehyde solution/ 0.2% (v/v) glutaraldehyde in vPBS containing EDTA-free protease inhibitor (Roche) was added to the resuspended cells ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections (3-5μm) from frontal cortex (BA 8/9) using the Nexes station automated system (Ventana Medical System Inc., Roche). Deparaffinised and rehydrated sections were pretreated and blocked ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
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bioRxiv - Bioengineering 2024Quote: ... The adaptor-ligated DNA was amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, Indianapolis, IN). The final libraries were quantitated on Qubit and the average size determined on the AATI Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed in TNT buffer (1% Triton X-100, 150 mM NaCl, 50 mM Tris HCl, pH 8) with protease inhibitors (Roche). 5 uL of whole cell lysate (approximately 40 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Plant Biology 2020Quote: ... Cells were then harvested and resuspended in 8 mL of Native Purification Buffer (50 mM NaH2PO4, 500 mM NaCl) supplemented with 8 mg lysozyme and a protease inhibitor tablet (Roche). The suspension was then incubated on ice for thirty minutes ...
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bioRxiv - Cell Biology 2019Quote: ... ground yeast powder was solubilized in 4 volumes of homogenization buffer (400 mM trisodium citrate, pH 8, 0.5% n-Dodecyl β-D-maltoside) and protease inhibitor cocktail (Roche). The soluble fraction was incubated with 10 μl magnetic beads (Dynabeads ...
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bioRxiv - Cell Biology 2019Quote: 10 heads from animals at 8 DAE were resuspended in 100 μl of Hepes/NaOH 50mM with proteases and phosphatases inhibitors (Roche), samples were disrupted using a mechanical tip following two rounds of sonication of 10 sec each ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, 1 mM TCEP, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested 48h after transfection and lysed in lysis buffer (50mM Tris-Cl pH 8, 150mM NaCl, 1% Triton, 1x Roche Complete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...
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bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and resuspended in Wash Buffer (150mM NaCl, 100mM Tris-HCl, pH 8) and a Complete protease inhibitor tablet was added (Roche). Cells were lysed by sonication (25% amplitude 1 sec on ...
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bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
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bioRxiv - Cell Biology 2021Quote: U2OS cells were seeded into 8-well Imaging Chamber CG (Zell-Kontakt) and transfected 48-72 h prior to imaging using XtremeGene HP (Roche) according to the manufacture’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 300 μL of Nuclei Lysis Buffer (Tris-HCl pH 8 50 mM, EDTA 10 mM, SDS 0.5% and protease inhibitor (Roche-04693132001). Nuclei were then sonicated (Diagenode bioruptor plus sonicatore ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation at 7000g at 4°C for 10 minutes and resuspended in 25 mL of cold TBS buffer (50 mM Tris-HCl, 500 mM NaCl, pH 8) with 5% glycerol and EDTA-free protease inhibitors cocktail (Roche). Cells were lysed by sonication and purified using Glutathione-Sepharose 4B (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... cells were recovered and lysed in 500 µl hypotonic lysis buffer (10 mM Tris-HCl pH 8, 10 mM KCl, 1× complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... collected and lysed with NP-40 extract buffer (50 mM Tris, pH 8, 150 mM NaCl, 1 % NP-40) containing protease inhibitors (#11873580001 Roche) and 30 µg of lysate was migrated in 10 % SDS-PAGE gels (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... the culture medium was replaced with serum-free medium 8 h prior to addition of IFN-γ (1000 IU; Roche). In experiments with Tofacitinib ...
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bioRxiv - Plant Biology 2021Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, CA) to reduce the likeliness of multiple identical reads due to preferential amplification ...
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bioRxiv - Plant Biology 2021Quote: ... For IPs 0.3 g of flower buds were ground in 1.5 ml of ice-cold lysis buffer (50 mM Tris-HCl pH 8, 50 mM NaCl, 1 % Triton X-100, supplemented with Roche cOmplete ...
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bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μl of the purified cDNA was mixed with 8 μl of a qPCR mixture [1.25x KAPA HiFi HotStart Readymix (KAPA Biosystems), 0.625 μM PCR lnPE1 primer ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
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bioRxiv - Genetics 2019Quote: ... cells were vortexed and passed through a syringe in ubiquitin lysis buffer (2% SDS, 150mM NaCl, 10 mM Tris HCl pH 8, 1 Roche protease inhibitor tablet ...