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Citations for Roche :
351 - 400 of 6830 citations for 7 Trifluoromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL of each sample was used for qPCR with 2 × Sybr Green (Roche) and primers for human RPLP2 (housekeeping gene) ...
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bioRxiv - Microbiology 2022Quote: ... (2) the quantitative Roche Spike Elecsys Anti-SARS-Cov-2 S assay (Roche, IND, USA), which measures spike total antibody concentrations ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples with RIN >7 were used for paired-end sequence library construction using the KAPA mRNA HyperPrep Kit (Roche # 08098123702). For ChIP samples ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Total RNA was isolated at day 7 or 14 of MSCs differentiation using the High Pure RNA Isolation kit (Roche Diagnostics). For tissues ...
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bioRxiv - Biophysics 2023Quote: ... Cells were pelleted and resuspended in 50 mM Tris (pH 7) supplemented with 500 μg/mL lysozyme and 1x cOmplete EDTA-free protease inhibitor cocktail (Roche, 11873580001). The pellet was incubated at 4 ºC while rotating end over end for 45 min.
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bioRxiv - Immunology 2021Quote: Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2′3′-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Plant Biology 2021Quote: ... 90 mM KCl, 2 mM EDTA, 0.5 mM spermidine, 0.2 mM spermine, 0.5 mM EGTA, 1× Roche Complete protease inhibitor cocktail). Nuclei were isolated by sucrose density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... mouse Hepa 1-6 and rat Fao hepatoma cells at 75% confluency were incubated with a mixture of oleate and palmitate (ratio 2:1, total concentration 1.2 mM) coupled to FA-free Bovine Serum Albumin (BSA) (Roche Applied Sciences). All fatty acid stocks were initially reconstituted in absolute ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours at room temperature with an anti-FL antibody conjugated with HRP (1/500, Roche Diagnostics) followed by reaction with TSA biotin reagents (Perkin Elmer ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
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bioRxiv - Molecular Biology 2019Quote: ... primary or re-amplified WTA products) added to 1 μl Expand Long Template Buffer 2 (Expand Long Template PCR System, Roche Diagnostics), 1 μl dNTPs (10 mM each) ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... plus phosphatase and protease inhibitors (2 mM NaVO4, 10 mM NaF, and 1 mM PMSF) and Complete protease inhibitor cocktail (Roche, CA). For tissue extracts ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins from mixed samples (14N-flg22/15N-mock and 14N-mock/15N-flg22) were extracted with IP buffer 2 with 1× Protease Inhibitor (Pierce) and phosphatase inhibitor (Roche, PhosSTOP), and centrifuged for 1700g for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Developmental Biology 2021Quote: ... The sample was incubated for 2 h with 2 μl of 20 mg/ml ProteinasK (Roche), and subjected to AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were blocked in 2% TNB (2% blocking reagent (Roche, REF 11 096 176 001) in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50 ...
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bioRxiv - Microbiology 2024Quote: ... interleukin-2 (IL-2; specific activity 10 U/ng) at concentration of 20 ng/mL (Roche) and phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Cancer Biology 2021Quote: ... dry milk or 2% BSA (Roche), membranes were probed with primary antibody (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA fraction V (Roche Diagnostics), 50 μM 2-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... 2 mg/mL Collagenase VIII (Roche) and 0.5 mg/mL DNAse I (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 pg of E.coli rRNA (Roche) was added as spike-in to equal volumes of RNA extracted from sucrose gradient fractions for the reverse transcription ...
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bioRxiv - Physiology 2019Quote: ... 2 mM EDTA with Complete (Roche) protease inhibitors) ...
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bioRxiv - Bioengineering 2021Quote: ... and 30U/ml hIL-2 (Roche). After 2 days ...
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bioRxiv - Plant Biology 2019Quote: ... 2 tablets Protease Inhibitor Cocktail (Roche) was added and cells were disrupted using an EmulsiFlex-C3 (Avestin) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of DNase I (Roche), and 1 μl of RNase OUT (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... 2 mg/ml collagenase D (Roche), and 1.5 mg/ml DNase I (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 2 protease inhibitor cocktail tablets (Roche) per 100 mL] ...
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bioRxiv - Biochemistry 2022Quote: ... 2 protease inhibitor cocktail tablets (Roche) per 100 mL solution] at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... we used 2% Blocking Reagent (Roche) in PBS with 0.1% Tween-20 ...