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Citations for Roche :
451 - 500 of 942 citations for 7 Methylidenebicyclo 3.3.1 nonan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: Batches of ten 2 day-old adult flies were crushed in 100 µl of lysis buffer (0.3% SDS in PBS plus one EDTA-free cOmplete™ Protease Inhibitor Cocktail tablet, Roche), incubated for 15 min and centrifuged at 15,000 gmax for 10 min (all manipulations at room temperature) ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Physiology 2022Quote: ... Fasting blood glucose was assessed by collecting blood from mice via tail puncture and using the one-touch Accu-Chek Aviva glucometer (Roche). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... with one modification to Homogenization Buffer preparation required in which cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... and the pellet was resuspended in 5 volumes of the lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche); buffer A ...
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bioRxiv - Systems Biology 2023Quote: ... Coverslips were washed with PBS three times for five minutes and blocked in a humid chamber for one hour at room temperature in blocking solution (1x Western Blocking reagent [Roche, 11921673001] diluted in PBS and supplemented with 0.3% Triton X-100) ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Microbiology 2023Quote: ... To prevent adherence of cells to one another by DNA leakage the suspension of cells was incubated with 600 Units of DNAse I (Roche) (30 mins ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown under induction for one generation and homogenised using bead beater (MPBio) in lysis buffer with protease inhibitor cocktail (Roche): 12 cycles of 7 m/sec ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Cell Biology 2020Quote: ... They were allowed to equilibrate in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 1 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) over night at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Immunology 2022Quote: ... Proliferating cells remaining in the cultures were further expanded by incubating for a further 7 days with 20 IU/mL IL-2 (Roche Life Science Products). After expansion ...
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bioRxiv - Microbiology 2022Quote: ... We excluded those with evidence of previous COVID-19 (defined as a positive NAAT COVID-19 test or presence of anti-nucleocapsid antibodies in the blood [Roche, IND, USA] 7) given the known differential impact on antibody responses post-vaccination.8 We also excluded cases for whom the second vaccine was >130 days after the first ...
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bioRxiv - Paleontology 2022Quote: ... The final libraries were quantified on an Applied Biosystems™ QuantStudio™ 7 Flex Real-Time PCR System using the KAPA SYBR® FAST ROX Low qPCR Master Mix for Illumina platforms (KAPA Biosystems, KK4873). DNA extracted from mammoth and sloth boluses were sequenced on an Illumina MiSeq using the 600-cycle v3 kit (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
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bioRxiv - Biochemistry 2021Quote: Cardiac myofibrils were prepared from left ventricular trabecular strips pre-stretched overnight to a sarcomere length of about 2 µm in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 2 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Pellet was resuspended in 7 mL of buffer (50 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to 20 strokes in Dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... Pellet was resuspended in 7 mL of buffer (50 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to 20 strokes in Dounce homogenizer ...
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bioRxiv - Genomics 2020Quote: ... Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) were used for the colorimetric detection of alkaline phosphatase activity ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 and 6 post transfection using WST-1 dye (Roche, Basel, Switzerland) as previously described 17.
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bioRxiv - Cell Biology 2019Quote: ... 100 μM ATP and supplemented with 1× phosphatase inhibitors cocktail 3 (Roche). Reactions were carried out at 30°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Neuroscience 2020Quote: ... Following resuspension in (3 ml) homogenization medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Blocking was then performed with DBPS containing 3% BSA (Roche, Cat#3116956001) and 1% Chicken serum (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... 1/3 of a Complete EDTA-free protease inhibitor cocktail tablet (Roche) and were lysed by five passes through an Avestin C3 homogenizer at 15-20,000 PSI ...
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bioRxiv - Cell Biology 2020Quote: ... The cartilage was then incubated in 3 mg/mL Collagenase D (Roche) in DMEM solution for two 45 minute periods and transferred to 0.5 mg/mL Collagenase D in DMEM solution supplemented with 3% Liberase TL (Sigma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5-bromo-4-chloro-3’-indolyphosphate (NBT/BCIP) and FastRed (Roche, Germany) staining was performed according to established published protocols62,63 using the following DIG and FITC labeled probes:
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bioRxiv - Molecular Biology 2023Quote: ... and 3 µl 10 mM dNTP (KAPA HiFi Kits from Roche, KK2502) were added to the annealed oligos ...
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bioRxiv - Developmental Biology 2024Quote: ... Beads were washed 3 times with lysis buffer containing proteinase inhibitors (Roche) and 7 times with lysis buffer without proteinase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... one volume of 2X TBS buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche Applied Science) was added to one volume of packed worms then added dropwise to liquid N2 to obtain frozen worm ‘popcorn’ ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
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bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...
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bioRxiv - Physiology 2021Quote: ... at time zero (T0) peripheral glucose level was measured at the tail using a One-Touch ultra-glucometer (AccuChek Sensor, Roche Diagnostics) and at T15 ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl, 20 mM Imidazole pH 8.0, one tablet of EDTA-free protease inhibitor [Roche; Product # COEDTAF-RO], DNaseI [Roche; Product #11284932001]) and lysed and centrifuged for 30 minutes at 18,000 x g at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM sodium dithionite) supplemented with 0.2 mg/mL bovine pancreatic deoxyribonuclease I and one cOmplete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were centrifuged and the pellet was resuspended in Resuspension Buffer (25 mM Tris HCl pH 7.5, 250 mM NaCl) supplemented with one tablet of cOmplete protease inhibitor (Roche, Cat # 04693159001). Cells were then lyzed using a high-pressure Emulsiflex C5 homogenizer (Avestin) ...
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bioRxiv - Physiology 2024Quote: Blood glucose levels were measured immediately in one drop of blood after the heart puncture with the Blood Glucose System Accu-Chek Performa (Roche Diagnostics).
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bioRxiv - Physiology 2024Quote: ... one lobe of dissected adipose tissue was homogenized with a Teflon pestle in RIPA buffer containing protease (cOmpleteTM Mini, Roche, Germany) and phosphatase (5 mM Na fluoride ...