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Citations for Roche :
351 - 400 of 1970 citations for 6 Pentyl 2H pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche). Cells were subsequently incubated on ice for 30 minutes ...
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bioRxiv - Genetics 2023Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche), as described previously (Kemaladewi et al. ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM MgCl2, 2 mM DTT, 2 µM leupeptin, 2 mM PMSF, 250 mM sucrose, and 1× PhosSTOP phosphatase inhibitors [Roche]). The homogenate was centrifuged for 15 min at 10,000 × g at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP) and cOmplete protease inhibitors (Roche). Cells were lysed by sonication and cell debris pelleted at 25,000 rpm for 40 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Mg(OAc)2 supplemented with phosphatase and protease inhibitors (Roche) (adapted from (29)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% blocking reagent (Roche), 20% heat inactivated goat serum and then incubated overnight with anti-DIG antibody (Roche ...
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bioRxiv - Biophysics 2019Quote: ... 6 μg of Prc was incubated with 0.6 μg of V8 protease (Roche) in 0.1 M Tris (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured 6 days post transfection using WST-1 reagent (Roche). Cells were incubated with 10 μl of WST-1 per 100 μl of media for 1 hour and absorbance was read at 450 and 630 nm ...
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bioRxiv - Plant Biology 2021Quote: ... with gene-specific primers (Supplementary Table 6) on a LightCycler 480 system (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Tumors were transferred into 6-well plates containing 0.25mg/mL LiberaseTL (Roche, 5401020001) and 0.33mg/mL DNase (Sigma-Aldrich DN25-10MG ...
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bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
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bioRxiv - Genomics 2023Quote: ... 6 μL of Ligation-1 mix (3.75 U T4 DNA ligase (Roche, 10799009001), 33.3 mM DTT (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems, KK2602). DNA library was sequenced using an Illumina NextSeq 500 75-cycle high output kit.
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis and RT-qPCR was carried out using One step RT QPCR reagent (Roche). Resulting signal was detected on Applied Biosystems Real Time PCR System ...
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bioRxiv - Synthetic Biology 2019Quote: ... previously supplemented with one tablet (per 10 ml of buffer volume) of complete protease inhibitor cocktail (Roche). Cell suspensions were incubated on ice for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... pH 8.0) containing 1 mg/mL lysozyme and one complete mini EDTA-free protease inhibitor pellet (Roche). Cell suspensions were incubated on ice for 20 min before sonication ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in one pellet volume of lysis buffer with complete EDTA-free protease inhibitor cocktail (Roche) before purification ...
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bioRxiv - Microbiology 2020Quote: ... One step real time RT-PCR was performed using the RNA Process Control Kit (Roche, Basel, Switzerland) with 5 μl of extracted RNA or directly from 5 μl of heated sample ...
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bioRxiv - Immunology 2022Quote: ... One microgram of total brain RNA was converted to cDNA (Transcriptor High Fidelity cDNA Synthesis Kit; Roche). A final volume of 20 μl of the cDNA reaction was diluted 1:3 in RNase-free water to be used in qRT-PCR ...
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bioRxiv - Genomics 2022Quote: One µg of DNA was used for whole genome bisulfite sequencing library preparation (KAPA Biosystems, Wilmington, MA), bisulphite conversion (Epitect Fast DNA Bisulfite Kit (Qiagen)) ...
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bioRxiv - Physiology 2019Quote: ... supplemented with protease inhibitor cocktail and one PhosSTOP tablet per 10ml (Cat.# 04 906 837 01, Roche) on ice ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription with ensuing PCR steps was performed using a Transcriptor One-Step RT-PCR kit (Roche) with EBOV-specific primers ...
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bioRxiv - Physiology 2021Quote: ... One µg of total RNA was then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche) as previously described [15] ...
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bioRxiv - Pathology 2021Quote: Real-time quantitative PCR was performed using KAPA PROBE FAST Universal One-Step qRT-PCR (Roche, Switzerland) and a specific pair of primers and a probe for amplification and detection of ToBRFV (Table S1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... One thousand ng of total RNA was used for retro-transcription (RT) (Expand™ Reverse Transcriptase, Roche). In the case of mixed infections ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500 ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) was prepared with one tablet of protein inhibitor cocktail (cOMPLETE Mini lacking EDTA, 11873580001, Roche) along with 15uL of RNAsin (N2615 ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (2 mM activated Na3VO4, 2 mM NaF and 1x PhosSTOP (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-Glutamine (R10) (Thermo 25030081) + 10 IU/mL IL-2 (Roche 11011456001) after CD3/CD28 stimulation ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20] and maintained for 2 hours in 2% blocking reagent (11096176001, Roche) in TNT ...
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bioRxiv - Genomics 2019Quote: ... Index tagged samples were amplified (6 cycles of PCR, KAPA HiFi kit, KAPA Biosystems), quantified (Accuclear dsDNA Quantitation Solution ...
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bioRxiv - Molecular Biology 2020Quote: ... and 6 M urea) in the presence of protease and phosphatase inhibitor cocktails (Roche). Mechanical homogenization of the tissue was performed using a Precellys 24 tissue homogenizer (Bertin Instruments ...
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bioRxiv - Plant Biology 2022Quote: ... The glucose released was measured using the hexokinase/glucose-6-phosphate dehydrogenase method (Roche). Starch content (in glucose equivalents ...
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bioRxiv - Plant Biology 2023Quote: ... and released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche).
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bioRxiv - Biochemistry 2024Quote: ... DNA vectors were transfected using FuGENE 6 transfection reagent (Roche Applied Science, Laval, QC) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM creatine phosphate (Roche), 10 ng/µL creatine kinase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% Blocking reagent (Roche, 11096176001), 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... using 2 % blocking reagent (Roche), followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750 ...
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bioRxiv - Microbiology 2020Quote: ... 2 U Lactate dehydrogenase (Roche) and 10 µg PGAM was pre-warmed to 30 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking reagent (Roche – 1096176001), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG-AP antibody (Roche – 11093274910 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2× Protease Inhibitor Cocktail (Roche), 2 mM 2-mercaptoethanol ...