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Citations for Roche :
401 - 450 of 7646 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... monocytes were harvested using ice-cold lysis buffer (1% Triton X-100, 2% SDS, 150 mM NaCl, 10 mM HEPES, 2 mM EDTA containing protease inhibitor cocktail - Roche). Cell lysates were heated at 100 °C for 5 min in the presence of Laemmli buffer (20% β-mercaptoethanol ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 20 mM KCl, 2 mM MgCl2, 1% TX-100, 40U Ribolock ml-2 and protease inhibitor cocktail, Roche) followed by clearing at 17 000 × g for 10 min at +4C° ...
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bioRxiv - Cell Biology 2023Quote: ... 2 M urea, 0.5% SDS, 1 mM EDTA pH8.0, 1 mM DTT, and 1× protease inhibitor cocktail [Roche, cat#4693132001]). Biotinylated RBPs were captured by affinity purification ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Plant Biology 2022Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 3 ml lysis buffer (PBS containing 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and cOmplete proteinase inhibitor [Roche Diagnostics]), lysed by ultrasonic treatment and incubated with 1.2 ml NeutrAvidin Agarose for 0.5 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Cell Biology 2022Quote: ... the dorsal skin of C57BL/6 neonates was collected and incubated overnight with 1 mg/mL collagenase/dispase (Roche). The dermis and epidermis were separated by incubating the skin with 0.25% trypsin/10 mM EDTA in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a 6 μl volume of 1 x Kapa Probe Fast Mastermix (Kapa Biosystems, Amsterdam, Netherlands). TaqMan assays (FAM labelled ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... lower hind limb muscles from C57BL/6 mice aged 6-8 weeks were dissected and digested with collagenase (Roche). Isolated cells were plated on matrigel-coated dishes and allowed to grow in DMEM containing 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... 3 mM ATP (Roche), 25 μg/ml MSU (InvivoGen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lumbar vertebrae 1 – 5 were incubated in 2% Collagenase P (Roche, Switzerland) for 30 minutes at 30° C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pH 7.5) with the addition of 2 mg·ml-1 collagenase (Roche, Indianapolis). Defolliculated oocytes were injected with ENaC cRNAs into the cytosol (25 ng per oocyte in 50 nl of RNase-free water ...
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bioRxiv - Neuroscience 2020Quote: ... nuclei were stained with 4,6 diamine-2-phenylindole (1:25000, DAPI, Roche) in PBS 1X for 1 minute ...
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bioRxiv - Biophysics 2023Quote: ... and protease inhibitor tablets (1 per 2 L culture, Roche, Basel, Switzerland) and lysed via sonication ...
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bioRxiv - Neuroscience 2023Quote: ... 1 or 2 mL of the cOmplete His-Tag purification resin (Roche) equilibrated with the solubilization buffer 2 was added ...
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bioRxiv - Microbiology 2024Quote: ... 2) step - 1:0.85 using Kapa HyperPure Beads (Roche, cat. no. 07983298001). Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... 80 µl of dephosphorylation solution (8 μl 10x dephosphorylation buffer, 2 μl RNasin Plus, 67 μl ddH2O, and 3 μl alkaline phosphatase (Roche, catalog no. 10713023001)) was added to the beads ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Cell Biology 2019Quote: ... 3) 2hr RT incubation in blocking solution (5% Sheep Serum, 1% Roche Blocking Buffer in PBST); 4 ...
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bioRxiv - Systems Biology 2023Quote: ... 500 mM NaCl and 3 mM Dithiothreitol (DTT)) supplemented with 1 mg deoxyribonuclease I (DNaseI, Roche) and protease inhibitor 4-(2-Aminoethyl ...
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bioRxiv - Cell Biology 2024Quote: ... with primer set #3 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BspEI and KpnII sites respectively ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) supplemented with one pellet EDTA-free protease inhibitor (Roche), and sonicated on ice for 5 × 30 s at 80% amplitude (0.5 s cycles ...
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bioRxiv - Genomics 2019Quote: ... one cOmplete EDTA-free EASYpack protease inhibitor tablet (Roche, Basel, Switzerland) was added to each cell pellet corresponding to 1 L of growth ...
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bioRxiv - Neuroscience 2019Quote: ... 50 mM ammonium bicarbonate and one complete mini protease inhibitor (Roche). The lysate was sonicated on ice using Bioruptor-Diagenode and centrifuged at 2500 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... One tablet of Roche cOmplete protease inhibitor was dissolved (Roche, 11697498001) in 50 mL of DI water to obtain a working solution of 50x protease inhibitor cocktail ...
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bioRxiv - Microbiology 2020Quote: ... One gel was blotted to a PVDF (Polyvinylidene fluoride) membrane (Roche) by semi-dry Western blotting ...
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bioRxiv - Biophysics 2022Quote: ... one crushed tab of Complete EDTA-free protease inhibitor cocktail (Roche) was added to ice-cold extraction buffer ...
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bioRxiv - Microbiology 2022Quote: ... and a protease inhibitor cocktail (Roche, one tablet/10 mL buffer)) ...
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bioRxiv - Biochemistry 2021Quote: ... one tablet per 50 mL of buffer (EDTA-free, Roche Diagnostics) and benzonase (E1014-25KU >250 Units/liter of culture)) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and one tablet of EDTA-free protease inhibitor (Roche) per 50 mL ...
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bioRxiv - Biochemistry 2021Quote: ... 10% (v/v) glycerol and one Complete protease inhibitor tablet (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... one Complete EDTA-free protease inhibitor cocktail tablet (Roche, Basel, Switzerland) and 1% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... and one complete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Cells were homogenized by inverting the tube several times until the mixture becomes very viscous (a 5 min incubation period at room temperature might be necessary to achieve viscosity) ...
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bioRxiv - Plant Biology 2022Quote: ... one tablet of cOmplete EDTA-free Protease Inhibitor Cocktail /50ml (Roche)) ...
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bioRxiv - Molecular Biology 2022Quote: ... one mM EDTA and supplemented with protease and phosphatase inhibitors (Roche)] ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 % glycerol) supplemented with one tablet of protease inhibitor cocktail (Roche) for every 10 mL of RIPA ...
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bioRxiv - Biochemistry 2023Quote: ... with one tablet of protease inhibitors (cOmplete Protease Inhibitor Cocktails, Roche) and 500 µl of 2mg/ml DNaseI (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... one EDTA-free mini-Complete protease inhibitor tablet (Roche, Basel, Switzerland) and DNase I ...
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bioRxiv - Biochemistry 2023Quote: ... aprotinin and one tablet complete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with complete protease inhibitors (one tablet per 25 mL: Roche), and 0.1% b-mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... and one complete EDTA-free protease inhibitor mixture tablet (Roche Diagnostics). The lysates were centrifuged at 20,000×g for 30 min at 4 °C to obtain the soluble protein fraction containing the expressed LZTR1 proteins ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Biophysics 2022Quote: ... the force was kept at 11 pN and Pif1 was added to the microfluidic chamber at a concentration of 6 nM and 1 mM ATP Lithium salt (Roche) in G4-Pif1 buffer ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were transfected with Fugene 6 (Roche). The day before transfection 5 x 105 N2A cells were plated on 6 cm plates ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 U/ml glucose-6-phosphate dehydrogenase (Roche), 2.5 U/ml hexokinase (Roche) ...